Neutrophils reduce the parasite burden in Leishmania (Leishmania) amazonensis-infected macrophages.

<h4>Background</h4>Studies on the role of neutrophils in Leishmania infection were mainly performed with L. (L) major, whereas less information is available for L. (L) amazonensis. Previous results from our laboratory showed a large infiltrate of neutrophils in the site of infection in a...

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Autores principales:	Erico Vinícius de Souza Carmo, Simone Katz, Clara Lúcia Barbiéri
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ed96e62b5a74ff6b80abda74c9e05f2
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spelling	oai:doaj.org-article:9ed96e62b5a74ff6b80abda74c9e05f22021-11-18T07:02:30ZNeutrophils reduce the parasite burden in Leishmania (Leishmania) amazonensis-infected macrophages.1932-620310.1371/journal.pone.0013815https://doaj.org/article/9ed96e62b5a74ff6b80abda74c9e05f22010-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21082032/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Studies on the role of neutrophils in Leishmania infection were mainly performed with L. (L) major, whereas less information is available for L. (L) amazonensis. Previous results from our laboratory showed a large infiltrate of neutrophils in the site of infection in a mouse strain resistant to L. (L.) amazonensis (C3H/HePas). In contrast, the susceptible strain (BALB/c) displayed a predominance of macrophages harboring a high number of amastigotes and very few neutrophils. These findings led us to investigate the interaction of inflammatory neutrophils with L. (L.) amazonensis-infected macrophages in vitro.<h4>Methodology/principal findings</h4>Mouse peritoneal macrophages infected with L. (L.) amazonensis were co-cultured with inflammatory neutrophils, and after four days, the infection was quantified microscopically. Data are representative of three experiments with similar results. The main findings were 1) intracellular parasites were efficiently destroyed in the co-cultures; 2) the leishmanicidal effect was similar when cells were obtained from mouse strains resistant (C3H/HePas) or susceptible (BALB/c) to L. (L.) amazonensis; 3) parasite destruction did not require contact between infected macrophages and neutrophils; 4) tumor necrosis factor alpha (TNF-α), neutrophil elastase and platelet activating factor (PAF) were involved with the leishmanicidal activity, and 5) destruction of the parasites did not depend on generation of oxygen or nitrogen radicals, indicating that parasite clearance did not involve the classical pathway of macrophage activation by TNF-α, as reported for other Leishmania species.<h4>Conclusions/significance</h4>The present results provide evidence that neutrophils in concert with macrophages play a previously unrecognized leishmanicidal effect on L. (L.) amazonensis. We believe these findings may help to understand the mechanisms involved in innate immunity in cutaneous infection by this Leishmania species.Erico Vinícius de Souza CarmoSimone KatzClara Lúcia BarbiériPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 11, p e13815 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erico Vinícius de Souza Carmo Simone Katz Clara Lúcia Barbiéri Neutrophils reduce the parasite burden in Leishmania (Leishmania) amazonensis-infected macrophages.
description	<h4>Background</h4>Studies on the role of neutrophils in Leishmania infection were mainly performed with L. (L) major, whereas less information is available for L. (L) amazonensis. Previous results from our laboratory showed a large infiltrate of neutrophils in the site of infection in a mouse strain resistant to L. (L.) amazonensis (C3H/HePas). In contrast, the susceptible strain (BALB/c) displayed a predominance of macrophages harboring a high number of amastigotes and very few neutrophils. These findings led us to investigate the interaction of inflammatory neutrophils with L. (L.) amazonensis-infected macrophages in vitro.<h4>Methodology/principal findings</h4>Mouse peritoneal macrophages infected with L. (L.) amazonensis were co-cultured with inflammatory neutrophils, and after four days, the infection was quantified microscopically. Data are representative of three experiments with similar results. The main findings were 1) intracellular parasites were efficiently destroyed in the co-cultures; 2) the leishmanicidal effect was similar when cells were obtained from mouse strains resistant (C3H/HePas) or susceptible (BALB/c) to L. (L.) amazonensis; 3) parasite destruction did not require contact between infected macrophages and neutrophils; 4) tumor necrosis factor alpha (TNF-α), neutrophil elastase and platelet activating factor (PAF) were involved with the leishmanicidal activity, and 5) destruction of the parasites did not depend on generation of oxygen or nitrogen radicals, indicating that parasite clearance did not involve the classical pathway of macrophage activation by TNF-α, as reported for other Leishmania species.<h4>Conclusions/significance</h4>The present results provide evidence that neutrophils in concert with macrophages play a previously unrecognized leishmanicidal effect on L. (L.) amazonensis. We believe these findings may help to understand the mechanisms involved in innate immunity in cutaneous infection by this Leishmania species.
format	article
author	Erico Vinícius de Souza Carmo Simone Katz Clara Lúcia Barbiéri
author_facet	Erico Vinícius de Souza Carmo Simone Katz Clara Lúcia Barbiéri
author_sort	Erico Vinícius de Souza Carmo
title	Neutrophils reduce the parasite burden in Leishmania (Leishmania) amazonensis-infected macrophages.
title_short	Neutrophils reduce the parasite burden in Leishmania (Leishmania) amazonensis-infected macrophages.
title_full	Neutrophils reduce the parasite burden in Leishmania (Leishmania) amazonensis-infected macrophages.
title_fullStr	Neutrophils reduce the parasite burden in Leishmania (Leishmania) amazonensis-infected macrophages.
title_full_unstemmed	Neutrophils reduce the parasite burden in Leishmania (Leishmania) amazonensis-infected macrophages.
title_sort	neutrophils reduce the parasite burden in leishmania (leishmania) amazonensis-infected macrophages.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/9ed96e62b5a74ff6b80abda74c9e05f2
work_keys_str_mv	AT ericoviniciusdesouzacarmo neutrophilsreducetheparasiteburdeninleishmanialeishmaniaamazonensisinfectedmacrophages AT simonekatz neutrophilsreducetheparasiteburdeninleishmanialeishmaniaamazonensisinfectedmacrophages AT claraluciabarbieri neutrophilsreducetheparasiteburdeninleishmanialeishmaniaamazonensisinfectedmacrophages
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Neutrophils reduce the parasite burden in Leishmania (Leishmania) amazonensis-infected macrophages.

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