Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species

Western blot (Wb) is considered to be the gold standard test for Trichinella infection serology, since this method allows specific Trichinella antigens to be distinguished from cross-reactive antigens. This is not the case with widely used antibody assay techniques - indirect immunofluorescence and...

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Autores principales:	Ilić Nataša, Gruden-Movsesijan Alisa, Živojinović Milena, Sofronić-Milosavljević Ljiljana
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2014
Materias:	band triad anti-trichinella antibodies trichinellosis western blot Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/9edc8dd4b98340508acd5fff640d2c99
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spelling	oai:doaj.org-article:9edc8dd4b98340508acd5fff640d2c992021-11-17T21:27:52ZCharacteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species1820-744810.2478/acve-2014-0004https://doaj.org/article/9edc8dd4b98340508acd5fff640d2c992014-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2014-0004https://doaj.org/toc/1820-7448Western blot (Wb) is considered to be the gold standard test for Trichinella infection serology, since this method allows specific Trichinella antigens to be distinguished from cross-reactive antigens. This is not the case with widely used antibody assay techniques - indirect immunofluorescence and ELISA - which are sensitive, but subject to crossreactions that make the interpretation of weakly positive results difficult. Application of Trichinella spiralis muscle larvae excretory-secretory (ES) antigens for the specific antibody detection in ELISA resulted in improved specificity compared to that of crude worm extract that was previously in use, but since production of ES has not yet been standardized, differences among laboratories occur. For this reason, the Wb profile of serum samples from different T. spiralis infected host species: human, horse, swine and dog, was investigated in the Serbian National Reference Laboratory for Trichinellosis (NRLT). The common feature of the obtained Wb profiles was the appearance of a triad of bands with molecular masses (Mw) of 45, 49, and 53 kDa. The very same triad was recognized by a monoclonal antibody (mAb) 7C2C5 specific for an immunodominant epitope unique to the muscle larvae stage of all species in the genus Trichinella. Inhibition studies confirmed that mAb and anti-Trichinella antibodies from sera competed for the same parasite epitope. Based on the obtained results, the NRLT introduced the recognition of the above mention triad as the basis for specific anti-Trichinella antibodies detection in the sera of infected hosts.Ilić NatašaGruden-Movsesijan AlisaŽivojinović MilenaSofronić-Milosavljević LjiljanaSciendoarticleband triadanti-trichinella antibodiestrichinellosiswestern blotVeterinary medicineSF600-1100ENActa Veterinaria, Vol 64, Iss 1, Pp 33-43 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	band triad anti-trichinella antibodies trichinellosis western blot Veterinary medicine SF600-1100
spellingShingle	band triad anti-trichinella antibodies trichinellosis western blot Veterinary medicine SF600-1100 Ilić Nataša Gruden-Movsesijan Alisa Živojinović Milena Sofronić-Milosavljević Ljiljana Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species
description	Western blot (Wb) is considered to be the gold standard test for Trichinella infection serology, since this method allows specific Trichinella antigens to be distinguished from cross-reactive antigens. This is not the case with widely used antibody assay techniques - indirect immunofluorescence and ELISA - which are sensitive, but subject to crossreactions that make the interpretation of weakly positive results difficult. Application of Trichinella spiralis muscle larvae excretory-secretory (ES) antigens for the specific antibody detection in ELISA resulted in improved specificity compared to that of crude worm extract that was previously in use, but since production of ES has not yet been standardized, differences among laboratories occur. For this reason, the Wb profile of serum samples from different T. spiralis infected host species: human, horse, swine and dog, was investigated in the Serbian National Reference Laboratory for Trichinellosis (NRLT). The common feature of the obtained Wb profiles was the appearance of a triad of bands with molecular masses (Mw) of 45, 49, and 53 kDa. The very same triad was recognized by a monoclonal antibody (mAb) 7C2C5 specific for an immunodominant epitope unique to the muscle larvae stage of all species in the genus Trichinella. Inhibition studies confirmed that mAb and anti-Trichinella antibodies from sera competed for the same parasite epitope. Based on the obtained results, the NRLT introduced the recognition of the above mention triad as the basis for specific anti-Trichinella antibodies detection in the sera of infected hosts.
format	article
author	Ilić Nataša Gruden-Movsesijan Alisa Živojinović Milena Sofronić-Milosavljević Ljiljana
author_facet	Ilić Nataša Gruden-Movsesijan Alisa Živojinović Milena Sofronić-Milosavljević Ljiljana
author_sort	Ilić Nataša
title	Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species
title_short	Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species
title_full	Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species
title_fullStr	Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species
title_full_unstemmed	Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species
title_sort	characteristic band pattern in western blots for specific detection of anti-trichinella spiralis antibodies in different host species
publisher	Sciendo
publishDate	2014
url	https://doaj.org/article/9edc8dd4b98340508acd5fff640d2c99
work_keys_str_mv	AT ilicnatasa characteristicbandpatterninwesternblotsforspecificdetectionofantitrichinellaspiralisantibodiesindifferenthostspecies AT grudenmovsesijanalisa characteristicbandpatterninwesternblotsforspecificdetectionofantitrichinellaspiralisantibodiesindifferenthostspecies AT zivojinovicmilena characteristicbandpatterninwesternblotsforspecificdetectionofantitrichinellaspiralisantibodiesindifferenthostspecies AT sofronicmilosavljevicljiljana characteristicbandpatterninwesternblotsforspecificdetectionofantitrichinellaspiralisantibodiesindifferenthostspecies
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Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species

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