Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.

Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front...

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Autores principales:	Ulrich Leischner, Anja Schierloh, Walter Zieglgänsberger, Hans-Ulrich Dodt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ee2fd5a05244aafa9ce36e4a85dae93
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spelling	oai:doaj.org-article:9ee2fd5a05244aafa9ce36e4a85dae932021-12-02T20:22:06ZFormalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.1932-620310.1371/journal.pone.0010391https://doaj.org/article/9ee2fd5a05244aafa9ce36e4a85dae932010-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20436930/?tool=EBIhttps://doaj.org/toc/1932-6203Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three-dimensional observation of cellular structures in various tissue types without complicated staining techniques.Ulrich LeischnerAnja SchierlohWalter ZieglgänsbergerHans-Ulrich DodtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 4, p e10391 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ulrich Leischner Anja Schierloh Walter Zieglgänsberger Hans-Ulrich Dodt Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.
description	Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three-dimensional observation of cellular structures in various tissue types without complicated staining techniques.
format	article
author	Ulrich Leischner Anja Schierloh Walter Zieglgänsberger Hans-Ulrich Dodt
author_facet	Ulrich Leischner Anja Schierloh Walter Zieglgänsberger Hans-Ulrich Dodt
author_sort	Ulrich Leischner
title	Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.
title_short	Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.
title_full	Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.
title_fullStr	Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.
title_full_unstemmed	Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.
title_sort	formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/9ee2fd5a05244aafa9ce36e4a85dae93
work_keys_str_mv	AT ulrichleischner formalininducedfluorescencerevealscellshapeandmorphologyinbiologicaltissuesamples AT anjaschierloh formalininducedfluorescencerevealscellshapeandmorphologyinbiologicaltissuesamples AT walterzieglgansberger formalininducedfluorescencerevealscellshapeandmorphologyinbiologicaltissuesamples AT hansulrichdodt formalininducedfluorescencerevealscellshapeandmorphologyinbiologicaltissuesamples
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Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.

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