High-resolution micro-CT for morphologic and quantitative assessment of the sinusoid in human cavernous hemangioma of the liver.

Hepatic sinusoid plays a vital role in human cavernous hemangioma of the liver (CHL), and its morphologic investigation facilitates the understanding of microcirculation mechanism and pathological change of CHL. However, precise anatomical view of the hepatic sinusoid has been limited by the resolut...

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Autores principales:	Jinghao Duan, Chunhong Hu, Hua Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ee4df2df2654b918a10ff2530a72fdf
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spelling	oai:doaj.org-article:9ee4df2df2654b918a10ff2530a72fdf2021-11-18T08:02:28ZHigh-resolution micro-CT for morphologic and quantitative assessment of the sinusoid in human cavernous hemangioma of the liver.1932-620310.1371/journal.pone.0053507https://doaj.org/article/9ee4df2df2654b918a10ff2530a72fdf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23308240/?tool=EBIhttps://doaj.org/toc/1932-6203Hepatic sinusoid plays a vital role in human cavernous hemangioma of the liver (CHL), and its morphologic investigation facilitates the understanding of microcirculation mechanism and pathological change of CHL. However, precise anatomical view of the hepatic sinusoid has been limited by the resolution and contrast available from existing imaging techniques. While liver biopsy has traditionally been the reliable method for the assessment of hepatic sinusoids, the invasiveness and sampling error are its inherent limitations. In this study, imaging of CHL samples was performed using in-line phase-contrast imaging (ILPCI) technique with synchrotron radiation. ILPCI allowed clear visualization of soft tissues and revealed structural details that were invisible to conventional radiography. Combining the computed tomography (CT) technique, ILPCI-CT was used to acquire the high-resolution micro-CT images of CHL, and three dimensional (3D) microstructures of hepatic sinusoids were provided for the morphologic depiction and quantitative assessment. Our study demonstrated that ILPCI-CT could substantially improve the radiographic contrast of CHL tissues in vitro with no contrast agent. ILPCI-CT yielded high-resolution micro-CT image of CHL sample at the micron scale, corresponding to information on actual structures revealed at histological section. The 3D visualization provided an excellent view of the hepatic sinusoid. The accurate view of individual hepatic sinusoid was achieved. The valuable morphological parameters of hepatic sinusoids, such as thrombi, diameters, surface areas and volumes, were measured. These parameters were of great importance in the evaluation of CHL, and they provided quantitative descriptors that characterized anatomical properties and pathological features of hepatic sinusoids. The results highlight the high degree of sensitivity of the ILPCI-CT technique and demonstrate the feasibility of accurate visualization of hepatic sinusoids. Moreover, there is a correlation between the CHL and the size or morphology of hepatic sinusoids, which offers a potential use in noninvasive study and analysis of CHL.Jinghao DuanChunhong HuHua ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53507 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinghao Duan Chunhong Hu Hua Chen High-resolution micro-CT for morphologic and quantitative assessment of the sinusoid in human cavernous hemangioma of the liver.
description	Hepatic sinusoid plays a vital role in human cavernous hemangioma of the liver (CHL), and its morphologic investigation facilitates the understanding of microcirculation mechanism and pathological change of CHL. However, precise anatomical view of the hepatic sinusoid has been limited by the resolution and contrast available from existing imaging techniques. While liver biopsy has traditionally been the reliable method for the assessment of hepatic sinusoids, the invasiveness and sampling error are its inherent limitations. In this study, imaging of CHL samples was performed using in-line phase-contrast imaging (ILPCI) technique with synchrotron radiation. ILPCI allowed clear visualization of soft tissues and revealed structural details that were invisible to conventional radiography. Combining the computed tomography (CT) technique, ILPCI-CT was used to acquire the high-resolution micro-CT images of CHL, and three dimensional (3D) microstructures of hepatic sinusoids were provided for the morphologic depiction and quantitative assessment. Our study demonstrated that ILPCI-CT could substantially improve the radiographic contrast of CHL tissues in vitro with no contrast agent. ILPCI-CT yielded high-resolution micro-CT image of CHL sample at the micron scale, corresponding to information on actual structures revealed at histological section. The 3D visualization provided an excellent view of the hepatic sinusoid. The accurate view of individual hepatic sinusoid was achieved. The valuable morphological parameters of hepatic sinusoids, such as thrombi, diameters, surface areas and volumes, were measured. These parameters were of great importance in the evaluation of CHL, and they provided quantitative descriptors that characterized anatomical properties and pathological features of hepatic sinusoids. The results highlight the high degree of sensitivity of the ILPCI-CT technique and demonstrate the feasibility of accurate visualization of hepatic sinusoids. Moreover, there is a correlation between the CHL and the size or morphology of hepatic sinusoids, which offers a potential use in noninvasive study and analysis of CHL.
format	article
author	Jinghao Duan Chunhong Hu Hua Chen
author_facet	Jinghao Duan Chunhong Hu Hua Chen
author_sort	Jinghao Duan
title	High-resolution micro-CT for morphologic and quantitative assessment of the sinusoid in human cavernous hemangioma of the liver.
title_short	High-resolution micro-CT for morphologic and quantitative assessment of the sinusoid in human cavernous hemangioma of the liver.
title_full	High-resolution micro-CT for morphologic and quantitative assessment of the sinusoid in human cavernous hemangioma of the liver.
title_fullStr	High-resolution micro-CT for morphologic and quantitative assessment of the sinusoid in human cavernous hemangioma of the liver.
title_full_unstemmed	High-resolution micro-CT for morphologic and quantitative assessment of the sinusoid in human cavernous hemangioma of the liver.
title_sort	high-resolution micro-ct for morphologic and quantitative assessment of the sinusoid in human cavernous hemangioma of the liver.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9ee4df2df2654b918a10ff2530a72fdf
work_keys_str_mv	AT jinghaoduan highresolutionmicroctformorphologicandquantitativeassessmentofthesinusoidinhumancavernoushemangiomaoftheliver AT chunhonghu highresolutionmicroctformorphologicandquantitativeassessmentofthesinusoidinhumancavernoushemangiomaoftheliver AT huachen highresolutionmicroctformorphologicandquantitativeassessmentofthesinusoidinhumancavernoushemangiomaoftheliver
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High-resolution micro-CT for morphologic and quantitative assessment of the sinusoid in human cavernous hemangioma of the liver.

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