Transcriptomic Analysis of Listeria monocytogenes in Response to Bile Under Aerobic and Anaerobic Conditions

Listeria monocytogenes is a gram-positive facultative anaerobic bacterium that causes the foodborne illness listeriosis. The pathogenesis of this bacterium depends on its survival in anaerobic, acidic, and bile conditions encountered throughout the gastrointestinal (GI) tract. This transcriptomics s...

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Autores principales:	Damayanti Chakravarty, Gyan Sahukhal, Mark Arick, Morgan L. Davis, Janet R. Donaldson
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Listeria monocytogenes transcriptomics anaerobiosis bile stress response anaerobic Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9ee62bbd63a4463c986aaf1bc7bd9c67
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spelling	oai:doaj.org-article:9ee62bbd63a4463c986aaf1bc7bd9c672021-11-11T09:21:09ZTranscriptomic Analysis of Listeria monocytogenes in Response to Bile Under Aerobic and Anaerobic Conditions1664-302X10.3389/fmicb.2021.754748https://doaj.org/article/9ee62bbd63a4463c986aaf1bc7bd9c672021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.754748/fullhttps://doaj.org/toc/1664-302XListeria monocytogenes is a gram-positive facultative anaerobic bacterium that causes the foodborne illness listeriosis. The pathogenesis of this bacterium depends on its survival in anaerobic, acidic, and bile conditions encountered throughout the gastrointestinal (GI) tract. This transcriptomics study was conducted to analyze the differences in transcript levels produced under conditions mimicking the GI tract. Changes in transcript levels were analyzed using RNA isolated from L. monocytogenes strain F2365 at both aerobic and anaerobic conditions, upon exposure to 0 and 1% bile at acidic and neutral pH. Transcripts corresponding to genes responsible for pathogenesis, cell wall associated proteins, DNA repair, transcription factors, and stress responses had variations in levels under the conditions tested. Upon exposure to anaerobiosis in acidic conditions, there were variations in the transcript levels for the virulence factors internalins, listeriolysin O, etc., as well as many histidine sensory kinases. These data indicate that the response to anaerobiosis differentially influences the transcription of several genes related to the survival of L. monocytogenes under acidic and bile conditions. Though further research is needed to decipher the role of oxygen in pathogenesis of L. monocytogenes, these data provide comprehensive information on how this pathogen responds to the GI tract.Damayanti ChakravartyGyan SahukhalMark ArickMorgan L. DavisJanet R. DonaldsonFrontiers Media S.A.articleListeria monocytogenestranscriptomicsanaerobiosisbilestress responseanaerobicMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Listeria monocytogenes transcriptomics anaerobiosis bile stress response anaerobic Microbiology QR1-502
spellingShingle	Listeria monocytogenes transcriptomics anaerobiosis bile stress response anaerobic Microbiology QR1-502 Damayanti Chakravarty Gyan Sahukhal Mark Arick Morgan L. Davis Janet R. Donaldson Transcriptomic Analysis of Listeria monocytogenes in Response to Bile Under Aerobic and Anaerobic Conditions
description	Listeria monocytogenes is a gram-positive facultative anaerobic bacterium that causes the foodborne illness listeriosis. The pathogenesis of this bacterium depends on its survival in anaerobic, acidic, and bile conditions encountered throughout the gastrointestinal (GI) tract. This transcriptomics study was conducted to analyze the differences in transcript levels produced under conditions mimicking the GI tract. Changes in transcript levels were analyzed using RNA isolated from L. monocytogenes strain F2365 at both aerobic and anaerobic conditions, upon exposure to 0 and 1% bile at acidic and neutral pH. Transcripts corresponding to genes responsible for pathogenesis, cell wall associated proteins, DNA repair, transcription factors, and stress responses had variations in levels under the conditions tested. Upon exposure to anaerobiosis in acidic conditions, there were variations in the transcript levels for the virulence factors internalins, listeriolysin O, etc., as well as many histidine sensory kinases. These data indicate that the response to anaerobiosis differentially influences the transcription of several genes related to the survival of L. monocytogenes under acidic and bile conditions. Though further research is needed to decipher the role of oxygen in pathogenesis of L. monocytogenes, these data provide comprehensive information on how this pathogen responds to the GI tract.
format	article
author	Damayanti Chakravarty Gyan Sahukhal Mark Arick Morgan L. Davis Janet R. Donaldson
author_facet	Damayanti Chakravarty Gyan Sahukhal Mark Arick Morgan L. Davis Janet R. Donaldson
author_sort	Damayanti Chakravarty
title	Transcriptomic Analysis of Listeria monocytogenes in Response to Bile Under Aerobic and Anaerobic Conditions
title_short	Transcriptomic Analysis of Listeria monocytogenes in Response to Bile Under Aerobic and Anaerobic Conditions
title_full	Transcriptomic Analysis of Listeria monocytogenes in Response to Bile Under Aerobic and Anaerobic Conditions
title_fullStr	Transcriptomic Analysis of Listeria monocytogenes in Response to Bile Under Aerobic and Anaerobic Conditions
title_full_unstemmed	Transcriptomic Analysis of Listeria monocytogenes in Response to Bile Under Aerobic and Anaerobic Conditions
title_sort	transcriptomic analysis of listeria monocytogenes in response to bile under aerobic and anaerobic conditions
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9ee62bbd63a4463c986aaf1bc7bd9c67
work_keys_str_mv	AT damayantichakravarty transcriptomicanalysisoflisteriamonocytogenesinresponsetobileunderaerobicandanaerobicconditions AT gyansahukhal transcriptomicanalysisoflisteriamonocytogenesinresponsetobileunderaerobicandanaerobicconditions AT markarick transcriptomicanalysisoflisteriamonocytogenesinresponsetobileunderaerobicandanaerobicconditions AT morganldavis transcriptomicanalysisoflisteriamonocytogenesinresponsetobileunderaerobicandanaerobicconditions AT janetrdonaldson transcriptomicanalysisoflisteriamonocytogenesinresponsetobileunderaerobicandanaerobicconditions
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Transcriptomic Analysis of Listeria monocytogenes in Response to Bile Under Aerobic and Anaerobic Conditions

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