Ethanolamine Is a New Anti-Prion Compound
Prion diseases are a group of fatal neurodegenerative disorders caused by accumulation of proteinaceous infectious particles, or prions, which mainly consist of the abnormally folded, amyloidogenic prion protein, designated PrP<sup>Sc</sup>. PrP<sup>Sc</sup> is produced throu...
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2021
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oai:doaj.org-article:9ee846da524b4800b896217172b508982021-11-11T17:11:57ZEthanolamine Is a New Anti-Prion Compound10.3390/ijms2221117421422-00671661-6596https://doaj.org/article/9ee846da524b4800b896217172b508982021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11742https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Prion diseases are a group of fatal neurodegenerative disorders caused by accumulation of proteinaceous infectious particles, or prions, which mainly consist of the abnormally folded, amyloidogenic prion protein, designated PrP<sup>Sc</sup>. PrP<sup>Sc</sup> is produced through conformational conversion of the cellular isoform of prion protein, PrP<sup>C</sup>, in the brain. To date, no effective therapies for prion diseases have been developed. In this study, we incidentally noticed that mouse neuroblastoma N2a cells persistently infected with 22L scrapie prions, termed N2aC24L1-3 cells, reduced PrP<sup>Sc</sup> levels when cultured in advanced Dulbecco’s modified eagle medium (DMEM) but not in classic DMEM. PrP<sup>C</sup> levels remained unchanged in prion-uninfected parent N2aC24 cells cultured in advanced DMEM. These results suggest that advanced DMEM may contain an anti-prion compound(s). We then successfully identified ethanolamine in advanced DMEM has an anti-prion activity. Ethanolamine reduced PrP<sup>Sc</sup> levels in N2aC24L1-3 cells, but not PrP<sup>C</sup> levels in N2aC24 cells. Also, oral administration of ethanolamine through drinking water delayed prion disease in mice intracerebrally inoculated with RML scrapie prions. These results suggest that ethanolamine could be a new anti-prion compound.Keiji UchiyamaHideyuki HaraJunji ChidaAgriani Dini PasianaMorikazu ImamuraTsuyoshi MoriHanae TakatsukiRyuichiro AtarashiSuehiro SakaguchiMDPI AGarticleprionsprion proteinprotein misfoldingneurodegenerationethanolaminetherapyBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11742, p 11742 (2021) |
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| topic |
prions prion protein protein misfolding neurodegeneration ethanolamine therapy Biology (General) QH301-705.5 Chemistry QD1-999 |
| spellingShingle |
prions prion protein protein misfolding neurodegeneration ethanolamine therapy Biology (General) QH301-705.5 Chemistry QD1-999 Keiji Uchiyama Hideyuki Hara Junji Chida Agriani Dini Pasiana Morikazu Imamura Tsuyoshi Mori Hanae Takatsuki Ryuichiro Atarashi Suehiro Sakaguchi Ethanolamine Is a New Anti-Prion Compound |
| description |
Prion diseases are a group of fatal neurodegenerative disorders caused by accumulation of proteinaceous infectious particles, or prions, which mainly consist of the abnormally folded, amyloidogenic prion protein, designated PrP<sup>Sc</sup>. PrP<sup>Sc</sup> is produced through conformational conversion of the cellular isoform of prion protein, PrP<sup>C</sup>, in the brain. To date, no effective therapies for prion diseases have been developed. In this study, we incidentally noticed that mouse neuroblastoma N2a cells persistently infected with 22L scrapie prions, termed N2aC24L1-3 cells, reduced PrP<sup>Sc</sup> levels when cultured in advanced Dulbecco’s modified eagle medium (DMEM) but not in classic DMEM. PrP<sup>C</sup> levels remained unchanged in prion-uninfected parent N2aC24 cells cultured in advanced DMEM. These results suggest that advanced DMEM may contain an anti-prion compound(s). We then successfully identified ethanolamine in advanced DMEM has an anti-prion activity. Ethanolamine reduced PrP<sup>Sc</sup> levels in N2aC24L1-3 cells, but not PrP<sup>C</sup> levels in N2aC24 cells. Also, oral administration of ethanolamine through drinking water delayed prion disease in mice intracerebrally inoculated with RML scrapie prions. These results suggest that ethanolamine could be a new anti-prion compound. |
| format |
article |
| author |
Keiji Uchiyama Hideyuki Hara Junji Chida Agriani Dini Pasiana Morikazu Imamura Tsuyoshi Mori Hanae Takatsuki Ryuichiro Atarashi Suehiro Sakaguchi |
| author_facet |
Keiji Uchiyama Hideyuki Hara Junji Chida Agriani Dini Pasiana Morikazu Imamura Tsuyoshi Mori Hanae Takatsuki Ryuichiro Atarashi Suehiro Sakaguchi |
| author_sort |
Keiji Uchiyama |
| title |
Ethanolamine Is a New Anti-Prion Compound |
| title_short |
Ethanolamine Is a New Anti-Prion Compound |
| title_full |
Ethanolamine Is a New Anti-Prion Compound |
| title_fullStr |
Ethanolamine Is a New Anti-Prion Compound |
| title_full_unstemmed |
Ethanolamine Is a New Anti-Prion Compound |
| title_sort |
ethanolamine is a new anti-prion compound |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/9ee846da524b4800b896217172b50898 |
| work_keys_str_mv |
AT keijiuchiyama ethanolamineisanewantiprioncompound AT hideyukihara ethanolamineisanewantiprioncompound AT junjichida ethanolamineisanewantiprioncompound AT agrianidinipasiana ethanolamineisanewantiprioncompound AT morikazuimamura ethanolamineisanewantiprioncompound AT tsuyoshimori ethanolamineisanewantiprioncompound AT hanaetakatsuki ethanolamineisanewantiprioncompound AT ryuichiroatarashi ethanolamineisanewantiprioncompound AT suehirosakaguchi ethanolamineisanewantiprioncompound |
| _version_ |
1718432135254114304 |