Expression profile of microRNAs in the testes of patients with Klinefelter syndrome

Abstract Klinefelter syndrome (KS) is the most common sex chromosome aneuploidy. A distinctive characteristic of KS is oligozoospermia. Despite multiple studies that have described the natural history of the degenerative process of germ cells in patients with KS, the molecular mechanisms that initia...

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Autores principales:	Marisol Ibarra-Ramírez, Geovana Calvo-Anguiano, José de Jesús Lugo-Trampe, Laura Elia Martínez-de-Villarreal, David Rodríguez-Torres, Manuel Nistal, Pilar González-Peramato
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ef4831d8d774443994ce2e6bb432e32
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spelling	oai:doaj.org-article:9ef4831d8d774443994ce2e6bb432e322021-12-02T15:39:40ZExpression profile of microRNAs in the testes of patients with Klinefelter syndrome10.1038/s41598-020-68294-72045-2322https://doaj.org/article/9ef4831d8d774443994ce2e6bb432e322020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68294-7https://doaj.org/toc/2045-2322Abstract Klinefelter syndrome (KS) is the most common sex chromosome aneuploidy. A distinctive characteristic of KS is oligozoospermia. Despite multiple studies that have described the natural history of the degenerative process of germ cells in patients with KS, the molecular mechanisms that initiate this process are not well characterized. MicroRNA (miRNA)-mediated post-transcriptional control mechanisms have been increasingly recognized as important regulators of spermatogenesis; however, only a few studies have evaluated the role of miRNAs in the gonadal failure of these patients. Here, we describe a differential expression profile for the miRNAs in testicular tissue samples taken from KS patients. We analysed testicular tissue samples from 4 KS patients and 5 control patients (obstructive azoospermia) through next-generation sequencing, which can provide information about the mechanisms involved in the degeneration of germ cells. A distinctive differential expression profile was identified for 166 miRNAs in the KS patients: 66 were upregulated, and 100 were downregulated. An interactome analysis was performed for 7 of the upregulated and the 20 downregulated miRNAs. The results showed that the target genes are involved in the development, proliferation, and differentiation processes of spermatogenesis, which may explain their role in the development of infertility. This is the first report of a miRNA expression profile generated from testicular tissue samples of KS patients.Marisol Ibarra-RamírezGeovana Calvo-AnguianoJosé de Jesús Lugo-TrampeLaura Elia Martínez-de-VillarrealDavid Rodríguez-TorresManuel NistalPilar González-PeramatoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marisol Ibarra-Ramírez Geovana Calvo-Anguiano José de Jesús Lugo-Trampe Laura Elia Martínez-de-Villarreal David Rodríguez-Torres Manuel Nistal Pilar González-Peramato Expression profile of microRNAs in the testes of patients with Klinefelter syndrome
description	Abstract Klinefelter syndrome (KS) is the most common sex chromosome aneuploidy. A distinctive characteristic of KS is oligozoospermia. Despite multiple studies that have described the natural history of the degenerative process of germ cells in patients with KS, the molecular mechanisms that initiate this process are not well characterized. MicroRNA (miRNA)-mediated post-transcriptional control mechanisms have been increasingly recognized as important regulators of spermatogenesis; however, only a few studies have evaluated the role of miRNAs in the gonadal failure of these patients. Here, we describe a differential expression profile for the miRNAs in testicular tissue samples taken from KS patients. We analysed testicular tissue samples from 4 KS patients and 5 control patients (obstructive azoospermia) through next-generation sequencing, which can provide information about the mechanisms involved in the degeneration of germ cells. A distinctive differential expression profile was identified for 166 miRNAs in the KS patients: 66 were upregulated, and 100 were downregulated. An interactome analysis was performed for 7 of the upregulated and the 20 downregulated miRNAs. The results showed that the target genes are involved in the development, proliferation, and differentiation processes of spermatogenesis, which may explain their role in the development of infertility. This is the first report of a miRNA expression profile generated from testicular tissue samples of KS patients.
format	article
author	Marisol Ibarra-Ramírez Geovana Calvo-Anguiano José de Jesús Lugo-Trampe Laura Elia Martínez-de-Villarreal David Rodríguez-Torres Manuel Nistal Pilar González-Peramato
author_facet	Marisol Ibarra-Ramírez Geovana Calvo-Anguiano José de Jesús Lugo-Trampe Laura Elia Martínez-de-Villarreal David Rodríguez-Torres Manuel Nistal Pilar González-Peramato
author_sort	Marisol Ibarra-Ramírez
title	Expression profile of microRNAs in the testes of patients with Klinefelter syndrome
title_short	Expression profile of microRNAs in the testes of patients with Klinefelter syndrome
title_full	Expression profile of microRNAs in the testes of patients with Klinefelter syndrome
title_fullStr	Expression profile of microRNAs in the testes of patients with Klinefelter syndrome
title_full_unstemmed	Expression profile of microRNAs in the testes of patients with Klinefelter syndrome
title_sort	expression profile of micrornas in the testes of patients with klinefelter syndrome
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9ef4831d8d774443994ce2e6bb432e32
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Expression profile of microRNAs in the testes of patients with Klinefelter syndrome

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