In vitro Apatite Mineralization, Degradability, Cytocompatibility and in vivo New Bone Formation and Vascularization of Bioactive Scaffold of Polybutylene Succinate/Magnesium Phosphate/Wheat Protein Ternary Composite

Qinghui Zhao1 12 23 3,* Hongming Tang1 12 23 3,* Lishu Ren4 4, Jie Wei4 4 1School of Life Sciences and Technology, Tongji University, Shanghai 200092, People’s Republic of China; 2Translational Medical Center for Stem Cell Therapy & Institute for Regenerative Medicine, Shanghai Eas...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhao Q, Tang H, Ren L, Wei J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	: polybutylene succinate composite scaffold cytocompatibility osteogenesis vascularization Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9ef99443328948299d77af07362e576b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9ef99443328948299d77af07362e576b
record_format	dspace
spelling	oai:doaj.org-article:9ef99443328948299d77af07362e576b2021-12-02T12:19:03ZIn vitro Apatite Mineralization, Degradability, Cytocompatibility and in vivo New Bone Formation and Vascularization of Bioactive Scaffold of Polybutylene Succinate/Magnesium Phosphate/Wheat Protein Ternary Composite1178-2013https://doaj.org/article/9ef99443328948299d77af07362e576b2020-09-01T00:00:00Zhttps://www.dovepress.com/in-vitro-apatite-mineralization-degradability-cytocompatibility-and-in-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Qinghui Zhao1 12 23 3,* Hongming Tang1 12 23 3,* Lishu Ren4 4, Jie Wei4 4 1School of Life Sciences and Technology, Tongji University, Shanghai 200092, People’s Republic of China; 2Translational Medical Center for Stem Cell Therapy & Institute for Regenerative Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, People’s Republic of China; 3Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai 200123, People’s Republic of China; 4Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qinghui Zhao Tel +86-21-38804518Email qinghui_zhao@126.comPurpose: A bioactive and degradable scaffold of ternary composite with good biocompatibility and osteogenesis was developed for bone tissue repair.Materials and Methods: Polybutylene succinate (PS:50 wt%), magnesium phosphate (MP:40 wt%) and wheat protein (WP:10 wt%) composite (PMWC) scaffold was fabricated, and the biological performances of PMWC were evaluated both in vitro and vivo in this study.Results: PMWC scaffold possessed not only interconnected macropores (400 μm to 600 μm) but also micropores (10 μm &sim; 20 μm) on the walls of macropores. Incorporation of MP into composite improved the apatite mineralization (bioactivity) of PMWC scaffold in simulated body fluid (SBF), and addition of WP into composite further enhanced the degradability of PMWC in PBS compared with the scaffold of PS (50 wt%)/MP (50 wt%) composite (PMC) and PS alone. In addition, the PMWC scaffold containing MP and WP significantly promoted the proliferation and differentiation of mouse pre-osteoblastic cell line (MC3T3-E1) cells. Moreover, the images from synchrotron radiation microcomputed tomography (SRmCT) and histological sections of the in vivo implantation suggested that the PMWC scaffold containing MP and WP prominently improved the new bone formation and ingrowth compared with PMC and PS. Furthermore, the immunohistochemical analysis further confirmed that the PMWC scaffold obviously promoted osteogenesis and vascularization in vivo compared with PMC and PS.Conclusion: This study demonstrated that the biocompatible PMWC scaffold with improved bioactivity and degradability significantly promoted the osteogenesis and vascularization in vivo, which would have a great potential to be applied for bone tissue repair.Keywords: polybutylene succinate, composite scaffold, cytocompatibility, osteogenesis, vascularizationZhao QTang HRen LWei JDove Medical Pressarticle: polybutylene succinatecomposite scaffoldcytocompatibilityosteogenesisvascularizationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 7279-7295 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	: polybutylene succinate composite scaffold cytocompatibility osteogenesis vascularization Medicine (General) R5-920
spellingShingle	: polybutylene succinate composite scaffold cytocompatibility osteogenesis vascularization Medicine (General) R5-920 Zhao Q Tang H Ren L Wei J In vitro Apatite Mineralization, Degradability, Cytocompatibility and in vivo New Bone Formation and Vascularization of Bioactive Scaffold of Polybutylene Succinate/Magnesium Phosphate/Wheat Protein Ternary Composite
description	Qinghui Zhao1 12 23 3,* Hongming Tang1 12 23 3,* Lishu Ren4 4, Jie Wei4 4 1School of Life Sciences and Technology, Tongji University, Shanghai 200092, People’s Republic of China; 2Translational Medical Center for Stem Cell Therapy & Institute for Regenerative Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, People’s Republic of China; 3Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai 200123, People’s Republic of China; 4Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qinghui Zhao Tel +86-21-38804518Email qinghui_zhao@126.comPurpose: A bioactive and degradable scaffold of ternary composite with good biocompatibility and osteogenesis was developed for bone tissue repair.Materials and Methods: Polybutylene succinate (PS:50 wt%), magnesium phosphate (MP:40 wt%) and wheat protein (WP:10 wt%) composite (PMWC) scaffold was fabricated, and the biological performances of PMWC were evaluated both in vitro and vivo in this study.Results: PMWC scaffold possessed not only interconnected macropores (400 μm to 600 μm) but also micropores (10 μm &sim; 20 μm) on the walls of macropores. Incorporation of MP into composite improved the apatite mineralization (bioactivity) of PMWC scaffold in simulated body fluid (SBF), and addition of WP into composite further enhanced the degradability of PMWC in PBS compared with the scaffold of PS (50 wt%)/MP (50 wt%) composite (PMC) and PS alone. In addition, the PMWC scaffold containing MP and WP significantly promoted the proliferation and differentiation of mouse pre-osteoblastic cell line (MC3T3-E1) cells. Moreover, the images from synchrotron radiation microcomputed tomography (SRmCT) and histological sections of the in vivo implantation suggested that the PMWC scaffold containing MP and WP prominently improved the new bone formation and ingrowth compared with PMC and PS. Furthermore, the immunohistochemical analysis further confirmed that the PMWC scaffold obviously promoted osteogenesis and vascularization in vivo compared with PMC and PS.Conclusion: This study demonstrated that the biocompatible PMWC scaffold with improved bioactivity and degradability significantly promoted the osteogenesis and vascularization in vivo, which would have a great potential to be applied for bone tissue repair.Keywords: polybutylene succinate, composite scaffold, cytocompatibility, osteogenesis, vascularization
format	article
author	Zhao Q Tang H Ren L Wei J
author_facet	Zhao Q Tang H Ren L Wei J
author_sort	Zhao Q
title	In vitro Apatite Mineralization, Degradability, Cytocompatibility and in vivo New Bone Formation and Vascularization of Bioactive Scaffold of Polybutylene Succinate/Magnesium Phosphate/Wheat Protein Ternary Composite
title_short	In vitro Apatite Mineralization, Degradability, Cytocompatibility and in vivo New Bone Formation and Vascularization of Bioactive Scaffold of Polybutylene Succinate/Magnesium Phosphate/Wheat Protein Ternary Composite
title_full	In vitro Apatite Mineralization, Degradability, Cytocompatibility and in vivo New Bone Formation and Vascularization of Bioactive Scaffold of Polybutylene Succinate/Magnesium Phosphate/Wheat Protein Ternary Composite
title_fullStr	In vitro Apatite Mineralization, Degradability, Cytocompatibility and in vivo New Bone Formation and Vascularization of Bioactive Scaffold of Polybutylene Succinate/Magnesium Phosphate/Wheat Protein Ternary Composite
title_full_unstemmed	In vitro Apatite Mineralization, Degradability, Cytocompatibility and in vivo New Bone Formation and Vascularization of Bioactive Scaffold of Polybutylene Succinate/Magnesium Phosphate/Wheat Protein Ternary Composite
title_sort	in vitro apatite mineralization, degradability, cytocompatibility and in vivo new bone formation and vascularization of bioactive scaffold of polybutylene succinate/magnesium phosphate/wheat protein ternary composite
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/9ef99443328948299d77af07362e576b
work_keys_str_mv	AT zhaoq invitroapatitemineralizationdegradabilitycytocompatibilityandinvivonewboneformationandvascularizationofbioactivescaffoldofpolybutylenesuccinatemagnesiumphosphatewheatproteinternarycomposite AT tangh invitroapatitemineralizationdegradabilitycytocompatibilityandinvivonewboneformationandvascularizationofbioactivescaffoldofpolybutylenesuccinatemagnesiumphosphatewheatproteinternarycomposite AT renl invitroapatitemineralizationdegradabilitycytocompatibilityandinvivonewboneformationandvascularizationofbioactivescaffoldofpolybutylenesuccinatemagnesiumphosphatewheatproteinternarycomposite AT weij invitroapatitemineralizationdegradabilitycytocompatibilityandinvivonewboneformationandvascularizationofbioactivescaffoldofpolybutylenesuccinatemagnesiumphosphatewheatproteinternarycomposite
_version_	1718394528984989696

In vitro Apatite Mineralization, Degradability, Cytocompatibility and in vivo New Bone Formation and Vascularization of Bioactive Scaffold of Polybutylene Succinate/Magnesium Phosphate/Wheat Protein Ternary Composite

Ejemplares similares