Evidence for OTUD-6B participation in B lymphocytes cell cycle after cytokine stimulation.

Deubiquitinating enzymes (DUBs) are important regulators of cell proliferation. Here we identified a functional deubiquitinating enzyme, ovarian tumor domain-containing 6B (OTUD-6B). Mutation of the conserved Cys residue abolished its deubiquitinating activity in vitro. Otud-6b expression was induce...

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Autores principales:	Zhongping Xu, Yufang Zheng, Yufei Zhu, Xiangyin Kong, Landian Hu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f00669ee7fe46228354f7bcadfa3317
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spelling	oai:doaj.org-article:9f00669ee7fe46228354f7bcadfa33172021-11-18T07:00:23ZEvidence for OTUD-6B participation in B lymphocytes cell cycle after cytokine stimulation.1932-620310.1371/journal.pone.0014514https://doaj.org/article/9f00669ee7fe46228354f7bcadfa33172011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21267069/?tool=EBIhttps://doaj.org/toc/1932-6203Deubiquitinating enzymes (DUBs) are important regulators of cell proliferation. Here we identified a functional deubiquitinating enzyme, ovarian tumor domain-containing 6B (OTUD-6B). Mutation of the conserved Cys residue abolished its deubiquitinating activity in vitro. Otud-6b expression was induced with cytokine stimulation in both mouse Ba/F3 cells and primary B lymphocytes followed a rapid decrease. This rapid decrease was partially facilitated by tristetraprolin (TTP) destabilization of Otud-6b mRNA through AU-rich motifs. Enforced expression of OTUD-6B in Ba/F3 cells could block cell proliferation by arresting cells in G1 phase. In addition, cyclin D2 level was down-regulated when OTUD-6B WT was overexpressed. Therefore, down-regulation of Otud-6b expression after prolonged cytokine stimulation may be required for cell proliferation in B lymphocytes.Zhongping XuYufang ZhengYufei ZhuXiangyin KongLandian HuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e14514 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhongping Xu Yufang Zheng Yufei Zhu Xiangyin Kong Landian Hu Evidence for OTUD-6B participation in B lymphocytes cell cycle after cytokine stimulation.
description	Deubiquitinating enzymes (DUBs) are important regulators of cell proliferation. Here we identified a functional deubiquitinating enzyme, ovarian tumor domain-containing 6B (OTUD-6B). Mutation of the conserved Cys residue abolished its deubiquitinating activity in vitro. Otud-6b expression was induced with cytokine stimulation in both mouse Ba/F3 cells and primary B lymphocytes followed a rapid decrease. This rapid decrease was partially facilitated by tristetraprolin (TTP) destabilization of Otud-6b mRNA through AU-rich motifs. Enforced expression of OTUD-6B in Ba/F3 cells could block cell proliferation by arresting cells in G1 phase. In addition, cyclin D2 level was down-regulated when OTUD-6B WT was overexpressed. Therefore, down-regulation of Otud-6b expression after prolonged cytokine stimulation may be required for cell proliferation in B lymphocytes.
format	article
author	Zhongping Xu Yufang Zheng Yufei Zhu Xiangyin Kong Landian Hu
author_facet	Zhongping Xu Yufang Zheng Yufei Zhu Xiangyin Kong Landian Hu
author_sort	Zhongping Xu
title	Evidence for OTUD-6B participation in B lymphocytes cell cycle after cytokine stimulation.
title_short	Evidence for OTUD-6B participation in B lymphocytes cell cycle after cytokine stimulation.
title_full	Evidence for OTUD-6B participation in B lymphocytes cell cycle after cytokine stimulation.
title_fullStr	Evidence for OTUD-6B participation in B lymphocytes cell cycle after cytokine stimulation.
title_full_unstemmed	Evidence for OTUD-6B participation in B lymphocytes cell cycle after cytokine stimulation.
title_sort	evidence for otud-6b participation in b lymphocytes cell cycle after cytokine stimulation.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/9f00669ee7fe46228354f7bcadfa3317
work_keys_str_mv	AT zhongpingxu evidenceforotud6bparticipationinblymphocytescellcycleaftercytokinestimulation AT yufangzheng evidenceforotud6bparticipationinblymphocytescellcycleaftercytokinestimulation AT yufeizhu evidenceforotud6bparticipationinblymphocytescellcycleaftercytokinestimulation AT xiangyinkong evidenceforotud6bparticipationinblymphocytescellcycleaftercytokinestimulation AT landianhu evidenceforotud6bparticipationinblymphocytescellcycleaftercytokinestimulation
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Evidence for OTUD-6B participation in B lymphocytes cell cycle after cytokine stimulation.

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