Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan

The study was performed to provide an overview of the molecular epidemiology of carbapenem-resistant <i>Acinetobacter baumannii</i> in Afghanistan isolated by the German military medical service during the Afghanistan conflict. A total of 18 isolates were collected between 2012 and 2018...

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Autores principales:	Paul G. Higgins, Meret Kniel, Sandra Rojak, Carsten Balczun, Holger Rohde, Hagen Frickmann, Ralf Matthias Hagen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Acinetobacter baumannii</i> molecular epidemiology carbapenem resistance Afghanistan surveillance Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9f0734c7ae8542d18a63f65637e7da11
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spelling	oai:doaj.org-article:9f0734c7ae8542d18a63f65637e7da112021-11-25T18:24:24ZMolecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan10.3390/microorganisms91122292076-2607https://doaj.org/article/9f0734c7ae8542d18a63f65637e7da112021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2229https://doaj.org/toc/2076-2607The study was performed to provide an overview of the molecular epidemiology of carbapenem-resistant <i>Acinetobacter baumannii</i> in Afghanistan isolated by the German military medical service during the Afghanistan conflict. A total of 18 isolates were collected between 2012 and 2018 at the microbiological laboratory of the field hospital in Camp Marmal near Mazar-e Sharif, Afghanistan, from Afghan patients. The isolates were subjected to phenotypic and genotypic differentiation and antimicrobial susceptibility testing as well as to a core genome multi-locus sequence typing (cgMLST) approach based on whole-genome next-generation sequence (wgNGS) data. Next to several sporadic isolates, four transmission clusters comprising strains from the international clonal lineages IC1, IC2, and IC9 were identified. Acquired carbapenem resistance was due to <i>bla</i><sub>OXA-23</sub> in 17/18 isolates, while genes mediating resistance against sulfonamides, macrolides, tetracyclines, and aminoglycosides were frequently identified as well. In conclusion, the assessment confirmed both the frequent occurrence of <i>A. baumannii</i> associated with outbreak events and a variety of different clones in Afghanistan. The fact that acquired carbapenem resistance was almost exclusively associated with <i>bla</i><sub>OXA-23</sub> may facilitate molecular resistance screening based on rapid molecular assays targeting this resistance determinant.Paul G. HigginsMeret KnielSandra RojakCarsten BalczunHolger RohdeHagen FrickmannRalf Matthias HagenMDPI AGarticle<i>Acinetobacter baumannii</i>molecular epidemiologycarbapenem resistanceAfghanistansurveillanceBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2229, p 2229 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Acinetobacter baumannii</i> molecular epidemiology carbapenem resistance Afghanistan surveillance Biology (General) QH301-705.5
spellingShingle	<i>Acinetobacter baumannii</i> molecular epidemiology carbapenem resistance Afghanistan surveillance Biology (General) QH301-705.5 Paul G. Higgins Meret Kniel Sandra Rojak Carsten Balczun Holger Rohde Hagen Frickmann Ralf Matthias Hagen Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan
description	The study was performed to provide an overview of the molecular epidemiology of carbapenem-resistant <i>Acinetobacter baumannii</i> in Afghanistan isolated by the German military medical service during the Afghanistan conflict. A total of 18 isolates were collected between 2012 and 2018 at the microbiological laboratory of the field hospital in Camp Marmal near Mazar-e Sharif, Afghanistan, from Afghan patients. The isolates were subjected to phenotypic and genotypic differentiation and antimicrobial susceptibility testing as well as to a core genome multi-locus sequence typing (cgMLST) approach based on whole-genome next-generation sequence (wgNGS) data. Next to several sporadic isolates, four transmission clusters comprising strains from the international clonal lineages IC1, IC2, and IC9 were identified. Acquired carbapenem resistance was due to <i>bla</i><sub>OXA-23</sub> in 17/18 isolates, while genes mediating resistance against sulfonamides, macrolides, tetracyclines, and aminoglycosides were frequently identified as well. In conclusion, the assessment confirmed both the frequent occurrence of <i>A. baumannii</i> associated with outbreak events and a variety of different clones in Afghanistan. The fact that acquired carbapenem resistance was almost exclusively associated with <i>bla</i><sub>OXA-23</sub> may facilitate molecular resistance screening based on rapid molecular assays targeting this resistance determinant.
format	article
author	Paul G. Higgins Meret Kniel Sandra Rojak Carsten Balczun Holger Rohde Hagen Frickmann Ralf Matthias Hagen
author_facet	Paul G. Higgins Meret Kniel Sandra Rojak Carsten Balczun Holger Rohde Hagen Frickmann Ralf Matthias Hagen
author_sort	Paul G. Higgins
title	Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan
title_short	Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan
title_full	Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan
title_fullStr	Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan
title_full_unstemmed	Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan
title_sort	molecular epidemiology of carbapenem-resistant <i>acinetobacter baumannii</i> strains isolated at the german military field laboratory in mazar-e sharif, afghanistan
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9f0734c7ae8542d18a63f65637e7da11
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Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan

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