Molecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus Aureus Isolated From Dairy Cattle In Egypt

The objectives of the study were to detect toxins and antiseptic resistance genes in Staphylococcus aureus isolated from cows with subclinical mastitis in Egypt. A total of 400 quarter milk samples (QMS) were collected from different dairy herds in which quaternary ammonium compounds (QAC) had been...

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Autores principales:	Ebtsam E.Z.Kotb, Jehan A. Gafer
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	mastitis quaternary ammonium compounds ( qac ) qms pcr s.aureus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.75411 https://doaj.org/article/9f081dce137d46c284c2065be7a28b9f
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spelling	oai:doaj.org-article:9f081dce137d46c284c2065be7a28b9f2021-12-02T11:43:50ZMolecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus Aureus Isolated From Dairy Cattle In Egypthttps://dx.doi.org/10.21608/javs.2020.754111687-40722090-3308https://doaj.org/article/9f081dce137d46c284c2065be7a28b9f2020-01-01T00:00:00Zhttps://javs.journals.ekb.eg/article_75411.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308The objectives of the study were to detect toxins and antiseptic resistance genes in Staphylococcus aureus isolated from cows with subclinical mastitis in Egypt. A total of 400 quarter milk samples (QMS) were collected from different dairy herds in which quaternary ammonium compounds (QAC) had been used as a disinfectant for more than 3years. The collected samples were subjected to bacterial investigation. S. aureus was successfully isolated confirmed by duplex PCR targeting 16S rRNA and nuc genes. Also determined their antibiogram and sensitivity to disinfectant. Genes of QAC(qacA/B), enterotoxins (Sea, Seb) and exfoliative toxins (ETB) were detected by simplex and multiplex PCR. Results of bacterial investigation revealed 103 (25.75%) S. aureus isolates. Results of antibiogram demonstrate that the most microbial antibiotics resistance were recorded for Penicillin G (85.7%) and Tetracycline (54.2%). While Gentamycin, Neomycin and Amoxicillin+ clavulanic acid show moderate resistance (21.4%, 10% and 7.1%) respectively, although Norfloxacin and Cephradine exhibited seldom resistance with high sensitivity of 95% and 94.3% respectively. Regarding the results of QAC sensitivity, only 8 isolates (7.76%) were resistant to benzalkonium chloride (BC) versus to 13 isolates (12.62%) harbour QAC gene could be detected by PCR with specific amplicon of 220bp corresponding to qacA/B. The results revealed Positive amplification of 102 bp specific for Sea gene in 19(18.44%) isolates and 164bp specific for Seb gene in 13(12.62%) isolates while there is no amplification was detected for etb gene. In conclusion, Antibiogram, as well as the identification of toxigenic and QAC genes in this study, may open another perspective in planning some alternative therapeutic strategies against multi resistances S. aureus mastitis. Monitoring cross-resistance between antibiotics and antiseptic should be further investigated.Ebtsam E.Z.KotbJehan A. GaferEgyptian Society for Animal Managementarticlemastitisquaternary ammonium compounds ( qac )qmspcrs.aureusZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 1, Pp 35-45 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	mastitis quaternary ammonium compounds ( qac ) qms pcr s.aureus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	mastitis quaternary ammonium compounds ( qac ) qms pcr s.aureus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Ebtsam E.Z.Kotb Jehan A. Gafer Molecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus Aureus Isolated From Dairy Cattle In Egypt
description	The objectives of the study were to detect toxins and antiseptic resistance genes in Staphylococcus aureus isolated from cows with subclinical mastitis in Egypt. A total of 400 quarter milk samples (QMS) were collected from different dairy herds in which quaternary ammonium compounds (QAC) had been used as a disinfectant for more than 3years. The collected samples were subjected to bacterial investigation. S. aureus was successfully isolated confirmed by duplex PCR targeting 16S rRNA and nuc genes. Also determined their antibiogram and sensitivity to disinfectant. Genes of QAC(qacA/B), enterotoxins (Sea, Seb) and exfoliative toxins (ETB) were detected by simplex and multiplex PCR. Results of bacterial investigation revealed 103 (25.75%) S. aureus isolates. Results of antibiogram demonstrate that the most microbial antibiotics resistance were recorded for Penicillin G (85.7%) and Tetracycline (54.2%). While Gentamycin, Neomycin and Amoxicillin+ clavulanic acid show moderate resistance (21.4%, 10% and 7.1%) respectively, although Norfloxacin and Cephradine exhibited seldom resistance with high sensitivity of 95% and 94.3% respectively. Regarding the results of QAC sensitivity, only 8 isolates (7.76%) were resistant to benzalkonium chloride (BC) versus to 13 isolates (12.62%) harbour QAC gene could be detected by PCR with specific amplicon of 220bp corresponding to qacA/B. The results revealed Positive amplification of 102 bp specific for Sea gene in 19(18.44%) isolates and 164bp specific for Seb gene in 13(12.62%) isolates while there is no amplification was detected for etb gene. In conclusion, Antibiogram, as well as the identification of toxigenic and QAC genes in this study, may open another perspective in planning some alternative therapeutic strategies against multi resistances S. aureus mastitis. Monitoring cross-resistance between antibiotics and antiseptic should be further investigated.
format	article
author	Ebtsam E.Z.Kotb Jehan A. Gafer
author_facet	Ebtsam E.Z.Kotb Jehan A. Gafer
author_sort	Ebtsam E.Z.Kotb
title	Molecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus Aureus Isolated From Dairy Cattle In Egypt
title_short	Molecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus Aureus Isolated From Dairy Cattle In Egypt
title_full	Molecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus Aureus Isolated From Dairy Cattle In Egypt
title_fullStr	Molecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus Aureus Isolated From Dairy Cattle In Egypt
title_full_unstemmed	Molecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus Aureus Isolated From Dairy Cattle In Egypt
title_sort	molecular detection of toxins and disinfectant resistance genes among staphylococcus aureus isolated from dairy cattle in egypt
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.75411 https://doaj.org/article/9f081dce137d46c284c2065be7a28b9f
work_keys_str_mv	AT ebtsamezkotb moleculardetectionoftoxinsanddisinfectantresistancegenesamongstaphylococcusaureusisolatedfromdairycattleinegypt AT jehanagafer moleculardetectionoftoxinsanddisinfectantresistancegenesamongstaphylococcusaureusisolatedfromdairycattleinegypt
_version_	1718395358830133248

Molecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus Aureus Isolated From Dairy Cattle In Egypt

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