The Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo

Abstract Christensenellaceae is a family of subdominant commensal bacteria found in humans. It is thought to play an important role in gut health by maintaining microbial symbiosis. Indeed, these bacteria occur at significantly lower levels or are absent in individuals suffering from inflammatory bo...

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Autores principales:	Camille Kropp, Katy Le Corf, Karima Relizani, Kevin Tambosco, Ccori Martinez, Florian Chain, Georges Rawadi, Philippe Langella, Sandrine P. Claus, Rebeca Martin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f0a79730e0d456f87c210b29cb9337f
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spelling	oai:doaj.org-article:9f0a79730e0d456f87c210b29cb9337f2021-12-02T15:02:57ZThe Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo10.1038/s41598-021-90885-12045-2322https://doaj.org/article/9f0a79730e0d456f87c210b29cb9337f2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90885-1https://doaj.org/toc/2045-2322Abstract Christensenellaceae is a family of subdominant commensal bacteria found in humans. It is thought to play an important role in gut health by maintaining microbial symbiosis. Indeed, these bacteria occur at significantly lower levels or are absent in individuals suffering from inflammatory bowel diseases (IBDs). Here, we explored if type species Christensenella minuta (strain: DSM 22607) could have the potential to help treat IBDs. We assessed key properties displayed by the bacterium using a combination of in vitro and in vivo assays. We found that while C. minuta is a strict anaerobe, it is also oxygen tolerant. Additionally, we observed that the species produces high levels of acetate and moderate levels of butyrate. We performed deep phenotyping using Biolog microarrays. Using human intestinal cell lines, we discovered that C. minuta demonstrated strong anti-inflammatory activity, resulting in reduced levels of proinflammatory IL-8 cytokines via the inhibition of the NF-κB signaling pathway. Furthermore, C. minuta protected intestinal epithelial integrity in vitro. Finally, in two distinct animal models of acute colitis, C. minuta prevented intestinal damage, reduced colonic inflammation, and promoted mucosal healing. Together, these results indicate that C. minuta has potent immunomodulatory properties, underscoring its potential use in innovative microbiome-based IBD biotherapies.Camille KroppKaty Le CorfKarima RelizaniKevin TamboscoCcori MartinezFlorian ChainGeorges RawadiPhilippe LangellaSandrine P. ClausRebeca MartinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Camille Kropp Katy Le Corf Karima Relizani Kevin Tambosco Ccori Martinez Florian Chain Georges Rawadi Philippe Langella Sandrine P. Claus Rebeca Martin The Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo
description	Abstract Christensenellaceae is a family of subdominant commensal bacteria found in humans. It is thought to play an important role in gut health by maintaining microbial symbiosis. Indeed, these bacteria occur at significantly lower levels or are absent in individuals suffering from inflammatory bowel diseases (IBDs). Here, we explored if type species Christensenella minuta (strain: DSM 22607) could have the potential to help treat IBDs. We assessed key properties displayed by the bacterium using a combination of in vitro and in vivo assays. We found that while C. minuta is a strict anaerobe, it is also oxygen tolerant. Additionally, we observed that the species produces high levels of acetate and moderate levels of butyrate. We performed deep phenotyping using Biolog microarrays. Using human intestinal cell lines, we discovered that C. minuta demonstrated strong anti-inflammatory activity, resulting in reduced levels of proinflammatory IL-8 cytokines via the inhibition of the NF-κB signaling pathway. Furthermore, C. minuta protected intestinal epithelial integrity in vitro. Finally, in two distinct animal models of acute colitis, C. minuta prevented intestinal damage, reduced colonic inflammation, and promoted mucosal healing. Together, these results indicate that C. minuta has potent immunomodulatory properties, underscoring its potential use in innovative microbiome-based IBD biotherapies.
format	article
author	Camille Kropp Katy Le Corf Karima Relizani Kevin Tambosco Ccori Martinez Florian Chain Georges Rawadi Philippe Langella Sandrine P. Claus Rebeca Martin
author_facet	Camille Kropp Katy Le Corf Karima Relizani Kevin Tambosco Ccori Martinez Florian Chain Georges Rawadi Philippe Langella Sandrine P. Claus Rebeca Martin
author_sort	Camille Kropp
title	The Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo
title_short	The Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo
title_full	The Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo
title_fullStr	The Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo
title_full_unstemmed	The Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo
title_sort	keystone commensal bacterium christensenella minuta dsm 22607 displays anti-inflammatory properties both in vitro and in vivo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9f0a79730e0d456f87c210b29cb9337f
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The Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo

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