Protective Effect of Dictyophora Polysaccharides on Sodium Arsenite-Induced Hepatotoxicity: A Proteomics Study
The purpose of this study is to understand the mechanism of sodium arsenite (NaAsO2)-induced apoptosis of L-02 human hepatic cells, and how Dictyophora polysaccharide (DIP) protects L-02 cells from arsenic-induced apoptosis. The results revealed that DIP pretreatment inhibited NaAsO2 induced L-02 ce...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:9f0ec1ee6053433baae509669a190c4a2021-12-01T05:15:02ZProtective Effect of Dictyophora Polysaccharides on Sodium Arsenite-Induced Hepatotoxicity: A Proteomics Study1663-981210.3389/fphar.2021.749035https://doaj.org/article/9f0ec1ee6053433baae509669a190c4a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.749035/fullhttps://doaj.org/toc/1663-9812The purpose of this study is to understand the mechanism of sodium arsenite (NaAsO2)-induced apoptosis of L-02 human hepatic cells, and how Dictyophora polysaccharide (DIP) protects L-02 cells from arsenic-induced apoptosis. The results revealed that DIP pretreatment inhibited NaAsO2 induced L-02 cells apoptosis by increasing anti-apoptotic Bcl-2 expression and decreasing pro-apoptotic Bax expression. Proteomic analysis showed that arsenic treatment disrupted the expression of metabolism and apoptosis associated proteins, including ribosomal proteins (RPs). After pretreatment with DIP, the expression levels of these proteins were reversed or restored. For the first time, it was observed that the significant decrease of cytoplasmic RPs and the increase of mitochondrial RPs were related to human normal cell apoptosis induced by arsenic. This is also the first report that the protective effect of DIP on cells was related to RPs. The results highlight the relationship between RPs and apoptosis, as well as the relationship between RPs and DIP attenuating arsenic-induced apoptosis.Ting HuTing HuTing HuLiming ShenQun HuangQun HuangChangyan WuHuajie ZhangQibing ZengQibing ZengQibing ZengGuoze WangGuoze WangShaofeng WeiShaofeng WeiShuling ZhangJun ZhangNaseer Ullah KhanXiangchun ShenPeng LuoPeng LuoPeng LuoPeng LuoFrontiers Media S.A.articledictyophora polysaccharidesNaAsO2apoptosishepatotoxicityproteomicsTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021) |
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| topic |
dictyophora polysaccharides NaAsO2 apoptosis hepatotoxicity proteomics Therapeutics. Pharmacology RM1-950 |
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dictyophora polysaccharides NaAsO2 apoptosis hepatotoxicity proteomics Therapeutics. Pharmacology RM1-950 Ting Hu Ting Hu Ting Hu Liming Shen Qun Huang Qun Huang Changyan Wu Huajie Zhang Qibing Zeng Qibing Zeng Qibing Zeng Guoze Wang Guoze Wang Shaofeng Wei Shaofeng Wei Shuling Zhang Jun Zhang Naseer Ullah Khan Xiangchun Shen Peng Luo Peng Luo Peng Luo Peng Luo Protective Effect of Dictyophora Polysaccharides on Sodium Arsenite-Induced Hepatotoxicity: A Proteomics Study |
| description |
The purpose of this study is to understand the mechanism of sodium arsenite (NaAsO2)-induced apoptosis of L-02 human hepatic cells, and how Dictyophora polysaccharide (DIP) protects L-02 cells from arsenic-induced apoptosis. The results revealed that DIP pretreatment inhibited NaAsO2 induced L-02 cells apoptosis by increasing anti-apoptotic Bcl-2 expression and decreasing pro-apoptotic Bax expression. Proteomic analysis showed that arsenic treatment disrupted the expression of metabolism and apoptosis associated proteins, including ribosomal proteins (RPs). After pretreatment with DIP, the expression levels of these proteins were reversed or restored. For the first time, it was observed that the significant decrease of cytoplasmic RPs and the increase of mitochondrial RPs were related to human normal cell apoptosis induced by arsenic. This is also the first report that the protective effect of DIP on cells was related to RPs. The results highlight the relationship between RPs and apoptosis, as well as the relationship between RPs and DIP attenuating arsenic-induced apoptosis. |
| format |
article |
| author |
Ting Hu Ting Hu Ting Hu Liming Shen Qun Huang Qun Huang Changyan Wu Huajie Zhang Qibing Zeng Qibing Zeng Qibing Zeng Guoze Wang Guoze Wang Shaofeng Wei Shaofeng Wei Shuling Zhang Jun Zhang Naseer Ullah Khan Xiangchun Shen Peng Luo Peng Luo Peng Luo Peng Luo |
| author_facet |
Ting Hu Ting Hu Ting Hu Liming Shen Qun Huang Qun Huang Changyan Wu Huajie Zhang Qibing Zeng Qibing Zeng Qibing Zeng Guoze Wang Guoze Wang Shaofeng Wei Shaofeng Wei Shuling Zhang Jun Zhang Naseer Ullah Khan Xiangchun Shen Peng Luo Peng Luo Peng Luo Peng Luo |
| author_sort |
Ting Hu |
| title |
Protective Effect of Dictyophora Polysaccharides on Sodium Arsenite-Induced Hepatotoxicity: A Proteomics Study |
| title_short |
Protective Effect of Dictyophora Polysaccharides on Sodium Arsenite-Induced Hepatotoxicity: A Proteomics Study |
| title_full |
Protective Effect of Dictyophora Polysaccharides on Sodium Arsenite-Induced Hepatotoxicity: A Proteomics Study |
| title_fullStr |
Protective Effect of Dictyophora Polysaccharides on Sodium Arsenite-Induced Hepatotoxicity: A Proteomics Study |
| title_full_unstemmed |
Protective Effect of Dictyophora Polysaccharides on Sodium Arsenite-Induced Hepatotoxicity: A Proteomics Study |
| title_sort |
protective effect of dictyophora polysaccharides on sodium arsenite-induced hepatotoxicity: a proteomics study |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/9f0ec1ee6053433baae509669a190c4a |
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