Enhancement of surface bioactivity on carbon fiber-reinforced polyether ether ketone via graphene modification

Jin-Hong Yan,1,* Chun-Hui Wang,2,* Ke-Wen Li,1,3,* Qi Zhang,1 Min Yang,1 Wei-Long Di-Wu,1 Ming Yan,1 Yue Song,1 Jing-Jing Ba,4 Long Bi,1 Yi-Sheng Han1 1Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, 2Department of Army Military Medical Fron...

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Autores principales:	Yan JH, Wang CH, Li KW, Zhang Q, Yang M, Di-Wu WL, Yan M, Song Y, Ba JJ, Bi L, Han YS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Graphene Carbon-fiber-reinforced polyether ether ketone Surface modification Bioactivity Osteointegration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9f123fbdc8ce4a6892215ea8c4a1046a
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spelling	oai:doaj.org-article:9f123fbdc8ce4a6892215ea8c4a1046a2021-12-02T02:34:32ZEnhancement of surface bioactivity on carbon fiber-reinforced polyether ether ketone via graphene modification1178-2013https://doaj.org/article/9f123fbdc8ce4a6892215ea8c4a1046a2018-06-01T00:00:00Zhttps://www.dovepress.com/enhancement-of-surface-bioactivity-on-carbon-fiber-reinforced-polyethe-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jin-Hong Yan,1,* Chun-Hui Wang,2,* Ke-Wen Li,1,3,* Qi Zhang,1 Min Yang,1 Wei-Long Di-Wu,1 Ming Yan,1 Yue Song,1 Jing-Jing Ba,4 Long Bi,1 Yi-Sheng Han1 1Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, 2Department of Army Military Medical Frontier Medical Service Brigade, Urumqi Ethnic Cadre College, Urumqi, Xinjiang Uyghur, 3Department of Orthopedics, Qinghai University Affiliated Hospital, Xining, Qinghai, 4Shandong Weigao Orthopedic Mechanics Laboratory, Weihai, Shandong, China *These authors contributed equally to this work Background and objective: The modulus of carbon fiber-reinforced polyether ether ketone (CFR-PEEK), a composite containing layers of carbon fiber sheets, can be precisely controlled to match bone. However, CFR-PEEK is biologically inert and cannot promote bone apposition. The objective of this study was to investigate whether graphene modification could enhance the bioactivity of CFR-PEEK.Methods and results: In vitro, the proliferation and differentiation of rat bone marrow stromal cells on scaffolds were quantified via cell-counting kit-8 assay and Western blotting analysis of osteoblast-specific proteins. Graphene modification significantly promoted bone marrow stromal cell proliferation and accelerated induced differentiation into osteogenic lineages compared to cells seeded onto nongraphene-coated CFR-PEEK. An in vivo rabbit extraarticular graft-to-bone healing model was established. At 4, 8, and 12 weeks after surgery, microcomputed tomography analyses and histological observations revealed significantly better microstructural parameters and higher average mineral apposition rates for graphene-modified CFR-PEEK implants than CFR-PEEK implants (P<0.05). van Gieson staining indicated more new bone was formed around graphene-modified CFR-PEEK implants than CFR-PEEK implants.Conclusion: Graphene may have considerable potential to enhance the bioactivity and osseointegration of CFR-PEEK implants for clinical applications. Keywords: graphene, carbon fiber-reinforced polyether ether ketone, surface modification, bioactivity, osseointegrationYan JHWang CHLi KWZhang QYang MDi-Wu WLYan MSong YBa JJBi LHan YSDove Medical PressarticleGrapheneCarbon-fiber-reinforced polyether ether ketoneSurface modificationBioactivityOsteointegrationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 3425-3440 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Graphene Carbon-fiber-reinforced polyether ether ketone Surface modification Bioactivity Osteointegration Medicine (General) R5-920
spellingShingle	Graphene Carbon-fiber-reinforced polyether ether ketone Surface modification Bioactivity Osteointegration Medicine (General) R5-920 Yan JH Wang CH Li KW Zhang Q Yang M Di-Wu WL Yan M Song Y Ba JJ Bi L Han YS Enhancement of surface bioactivity on carbon fiber-reinforced polyether ether ketone via graphene modification
description	Jin-Hong Yan,1,* Chun-Hui Wang,2,* Ke-Wen Li,1,3,* Qi Zhang,1 Min Yang,1 Wei-Long Di-Wu,1 Ming Yan,1 Yue Song,1 Jing-Jing Ba,4 Long Bi,1 Yi-Sheng Han1 1Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, 2Department of Army Military Medical Frontier Medical Service Brigade, Urumqi Ethnic Cadre College, Urumqi, Xinjiang Uyghur, 3Department of Orthopedics, Qinghai University Affiliated Hospital, Xining, Qinghai, 4Shandong Weigao Orthopedic Mechanics Laboratory, Weihai, Shandong, China *These authors contributed equally to this work Background and objective: The modulus of carbon fiber-reinforced polyether ether ketone (CFR-PEEK), a composite containing layers of carbon fiber sheets, can be precisely controlled to match bone. However, CFR-PEEK is biologically inert and cannot promote bone apposition. The objective of this study was to investigate whether graphene modification could enhance the bioactivity of CFR-PEEK.Methods and results: In vitro, the proliferation and differentiation of rat bone marrow stromal cells on scaffolds were quantified via cell-counting kit-8 assay and Western blotting analysis of osteoblast-specific proteins. Graphene modification significantly promoted bone marrow stromal cell proliferation and accelerated induced differentiation into osteogenic lineages compared to cells seeded onto nongraphene-coated CFR-PEEK. An in vivo rabbit extraarticular graft-to-bone healing model was established. At 4, 8, and 12 weeks after surgery, microcomputed tomography analyses and histological observations revealed significantly better microstructural parameters and higher average mineral apposition rates for graphene-modified CFR-PEEK implants than CFR-PEEK implants (P<0.05). van Gieson staining indicated more new bone was formed around graphene-modified CFR-PEEK implants than CFR-PEEK implants.Conclusion: Graphene may have considerable potential to enhance the bioactivity and osseointegration of CFR-PEEK implants for clinical applications. Keywords: graphene, carbon fiber-reinforced polyether ether ketone, surface modification, bioactivity, osseointegration
format	article
author	Yan JH Wang CH Li KW Zhang Q Yang M Di-Wu WL Yan M Song Y Ba JJ Bi L Han YS
author_facet	Yan JH Wang CH Li KW Zhang Q Yang M Di-Wu WL Yan M Song Y Ba JJ Bi L Han YS
author_sort	Yan JH
title	Enhancement of surface bioactivity on carbon fiber-reinforced polyether ether ketone via graphene modification
title_short	Enhancement of surface bioactivity on carbon fiber-reinforced polyether ether ketone via graphene modification
title_full	Enhancement of surface bioactivity on carbon fiber-reinforced polyether ether ketone via graphene modification
title_fullStr	Enhancement of surface bioactivity on carbon fiber-reinforced polyether ether ketone via graphene modification
title_full_unstemmed	Enhancement of surface bioactivity on carbon fiber-reinforced polyether ether ketone via graphene modification
title_sort	enhancement of surface bioactivity on carbon fiber-reinforced polyether ether ketone via graphene modification
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/9f123fbdc8ce4a6892215ea8c4a1046a
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Enhancement of surface bioactivity on carbon fiber-reinforced polyether ether ketone via graphene modification

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