Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.

Ever since iron oxide nanoparticles have been recognized as promising scaffolds for biomedical applications, their surface functionalization has become even more important. We report the synthesis of a novel polyethylene glycol-based ligand that combines multiple advantageous properties for these ap...

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Autores principales:	Maarten Bloemen, Thomas Van Stappen, Pieter Willot, Jeroen Lammertyn, Guy Koeckelberghs, Nick Geukens, Ann Gils, Thierry Verbiest
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f12c55396a0435787d7a909cc8f3f8c
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spelling	oai:doaj.org-article:9f12c55396a0435787d7a909cc8f3f8c2021-11-25T05:58:14ZHeterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.1932-620310.1371/journal.pone.0109475https://doaj.org/article/9f12c55396a0435787d7a909cc8f3f8c2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109475https://doaj.org/toc/1932-6203Ever since iron oxide nanoparticles have been recognized as promising scaffolds for biomedical applications, their surface functionalization has become even more important. We report the synthesis of a novel polyethylene glycol-based ligand that combines multiple advantageous properties for these applications. The ligand is covalently bound to the surface via a siloxane group, while its polyethylene glycol backbone significantly improves the colloidal stability of the particle in complex environments. End-capping the molecule with a carboxylic acid introduces a variety of coupling chemistry possibilities. In this study an antibody targeting plasminogen activator inhibitor-1 was coupled to the surface and its presence and binding activity was assessed by enzyme-linked immunosorbent assay and surface plasmon resonance experiments. The results indicate that the ligand has high potential towards biomedical applications where colloidal stability and advanced functionality is crucial.Maarten BloemenThomas Van StappenPieter WillotJeroen LammertynGuy KoeckelberghsNick GeukensAnn GilsThierry VerbiestPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109475 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maarten Bloemen Thomas Van Stappen Pieter Willot Jeroen Lammertyn Guy Koeckelberghs Nick Geukens Ann Gils Thierry Verbiest Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.
description	Ever since iron oxide nanoparticles have been recognized as promising scaffolds for biomedical applications, their surface functionalization has become even more important. We report the synthesis of a novel polyethylene glycol-based ligand that combines multiple advantageous properties for these applications. The ligand is covalently bound to the surface via a siloxane group, while its polyethylene glycol backbone significantly improves the colloidal stability of the particle in complex environments. End-capping the molecule with a carboxylic acid introduces a variety of coupling chemistry possibilities. In this study an antibody targeting plasminogen activator inhibitor-1 was coupled to the surface and its presence and binding activity was assessed by enzyme-linked immunosorbent assay and surface plasmon resonance experiments. The results indicate that the ligand has high potential towards biomedical applications where colloidal stability and advanced functionality is crucial.
format	article
author	Maarten Bloemen Thomas Van Stappen Pieter Willot Jeroen Lammertyn Guy Koeckelberghs Nick Geukens Ann Gils Thierry Verbiest
author_facet	Maarten Bloemen Thomas Van Stappen Pieter Willot Jeroen Lammertyn Guy Koeckelberghs Nick Geukens Ann Gils Thierry Verbiest
author_sort	Maarten Bloemen
title	Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.
title_short	Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.
title_full	Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.
title_fullStr	Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.
title_full_unstemmed	Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.
title_sort	heterobifunctional peg ligands for bioconjugation reactions on iron oxide nanoparticles.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9f12c55396a0435787d7a909cc8f3f8c
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Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.

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