Liver Regeneration and New Technical Possibilities by Two-Photon Based Intravital Imaging

One of the outstanding features of the liver is its enormous regeneration capacity. Compared to other solid organs, such as kidney, pancreas, heart or brain, the liver shows a superior capacity to regenerate. Probably, this regeneration capacity has evolved during ‘animal plant warfare’, when plants...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ahmed Ghallab, Jan G. Hengstler
Formato:	article
Lenguaje:	EN
Publicado:	South Valley University 2018
Materias:	Agriculture S Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/9f13b1789d7c41c8a883c9dcb6ee85f2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9f13b1789d7c41c8a883c9dcb6ee85f2
record_format	dspace
spelling	oai:doaj.org-article:9f13b1789d7c41c8a883c9dcb6ee85f22021-12-02T00:21:38ZLiver Regeneration and New Technical Possibilities by Two-Photon Based Intravital Imaging2535-18262535-1877https://doaj.org/article/9f13b1789d7c41c8a883c9dcb6ee85f22018-07-01T00:00:00Zhttps://portal.svu.edu.eg/svu-src/index.php/ijvs/article/view/17https://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1877One of the outstanding features of the liver is its enormous regeneration capacity. Compared to other solid organs, such as kidney, pancreas, heart or brain, the liver shows a superior capacity to regenerate. Probably, this regeneration capacity has evolved during ‘animal plant warfare’, when plants protected themselves from herbivores by new toxins and herbivores responded by novel detoxifying enzymes and efficient hepatic regeneration. Control mechanisms of liver regeneration have attracted scientists since decades. One limitation that has hampered progress is the lack of possibilities or real-time observations of cellular and subcellular processes in the regenerating liver without removing the organ for analysis. This has now become possible by the introduction of an improved technology of two-photon based intravital imaging. This technology allows the possibility to perform real-time imaging of the intact liver in anesthetized mice. Resolution is close to the theoretically possible 200 nm and therefore allows imaging of organelles and vesicles. Also, imaging of fast processes in the millisecond range is possible. Using available fluorescent reporter mouse systems it is possible to visualize all resident cell types of the liver, such as hepatocytes, Kupffer cells, stellate cells and sinusoidal endothelial cells. Furthermore, infiltrating immune cells can be imaged during liver injury and regeneration using cell-specific antibodies or reporter mice. This minireview presents some of the possibilities of intravital imaging and its applicability for research in the field of liver regeneration.Ahmed GhallabJan G. HengstlerSouth Valley UniversityarticleAgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 1, Iss 1, Pp 4-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Agriculture S Veterinary medicine SF600-1100
spellingShingle	Agriculture S Veterinary medicine SF600-1100 Ahmed Ghallab Jan G. Hengstler Liver Regeneration and New Technical Possibilities by Two-Photon Based Intravital Imaging
description	One of the outstanding features of the liver is its enormous regeneration capacity. Compared to other solid organs, such as kidney, pancreas, heart or brain, the liver shows a superior capacity to regenerate. Probably, this regeneration capacity has evolved during ‘animal plant warfare’, when plants protected themselves from herbivores by new toxins and herbivores responded by novel detoxifying enzymes and efficient hepatic regeneration. Control mechanisms of liver regeneration have attracted scientists since decades. One limitation that has hampered progress is the lack of possibilities or real-time observations of cellular and subcellular processes in the regenerating liver without removing the organ for analysis. This has now become possible by the introduction of an improved technology of two-photon based intravital imaging. This technology allows the possibility to perform real-time imaging of the intact liver in anesthetized mice. Resolution is close to the theoretically possible 200 nm and therefore allows imaging of organelles and vesicles. Also, imaging of fast processes in the millisecond range is possible. Using available fluorescent reporter mouse systems it is possible to visualize all resident cell types of the liver, such as hepatocytes, Kupffer cells, stellate cells and sinusoidal endothelial cells. Furthermore, infiltrating immune cells can be imaged during liver injury and regeneration using cell-specific antibodies or reporter mice. This minireview presents some of the possibilities of intravital imaging and its applicability for research in the field of liver regeneration.
format	article
author	Ahmed Ghallab Jan G. Hengstler
author_facet	Ahmed Ghallab Jan G. Hengstler
author_sort	Ahmed Ghallab
title	Liver Regeneration and New Technical Possibilities by Two-Photon Based Intravital Imaging
title_short	Liver Regeneration and New Technical Possibilities by Two-Photon Based Intravital Imaging
title_full	Liver Regeneration and New Technical Possibilities by Two-Photon Based Intravital Imaging
title_fullStr	Liver Regeneration and New Technical Possibilities by Two-Photon Based Intravital Imaging
title_full_unstemmed	Liver Regeneration and New Technical Possibilities by Two-Photon Based Intravital Imaging
title_sort	liver regeneration and new technical possibilities by two-photon based intravital imaging
publisher	South Valley University
publishDate	2018
url	https://doaj.org/article/9f13b1789d7c41c8a883c9dcb6ee85f2
work_keys_str_mv	AT ahmedghallab liverregenerationandnewtechnicalpossibilitiesbytwophotonbasedintravitalimaging AT janghengstler liverregenerationandnewtechnicalpossibilitiesbytwophotonbasedintravitalimaging
_version_	1718403818921656320

Liver Regeneration and New Technical Possibilities by Two-Photon Based Intravital Imaging

Ejemplares similares