Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions
Abstract Background Solid-state fermentation is a fungal culture technique used to produce compounds and products of industrial interest. The growth behaviour of filamentous fungi on solid media is challenging to study due to the intermixity of the substrate and the growing organism. Several strateg...
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oai:doaj.org-article:9f1c7590eae34bed85eef52b5146b78a2021-11-21T12:05:32ZMorphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions10.1186/s12866-021-02350-81471-2180https://doaj.org/article/9f1c7590eae34bed85eef52b5146b78a2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12866-021-02350-8https://doaj.org/toc/1471-2180Abstract Background Solid-state fermentation is a fungal culture technique used to produce compounds and products of industrial interest. The growth behaviour of filamentous fungi on solid media is challenging to study due to the intermixity of the substrate and the growing organism. Several strategies are available to measure indirectly the fungal biomass during the fermentation such as following the biochemical production of mycelium-specific components or microscopic observation. The microscopic observation of the development of the mycelium, on lignocellulosic substrate, has not been reported. In this study, we set up an experimental protocol based on microscopy and image processing through which we investigated the growth pattern of Phanerochaete chrysosporium on different Miscanthus x giganteus biomass fractions. Results Object coalescence, the occupied surface area, and radial expansion of the colony were measured in time. The substrate was sterilized by autoclaving, which could be considered a type of pre-treatment. The fastest growth rate was measured on the unfractionated biomass, followed by the soluble fraction of the biomass, then the residual solid fractions. The growth rate on the different fractions of the substrate was additive, suggesting that both the solid and soluble fractions were used by the fungus. Based on the FTIR analysis, there were differences in composition between the solid and soluble fractions of the substrate, but the main components for growth were always present. We propose using this novel method for measuring the very initial fungal growth by following the variation of the number of objects over time. Once growth is established, the growth can be followed by measurement of the occupied surface by the mycelium. Conclusion Our data showed that the growth was affected from the very beginning by the nature of the substrate. The most extensive colonization of the surface was observed with the unfractionated substrate containing both soluble and solid components. The methodology was practical and may be applied to investigate the growth of other fungi, including the influence of environmental parameters on the fungal growth.Hassan KhalilEstelle LeginBernard KurekPatrick PerreBehnam TaidiBMCarticleMiscanthus x giganteusSolid-state fermentationPhanerochaete chrysosporiumMicroscopy and image processingSpore germinationMycelial growthMicrobiologyQR1-502ENBMC Microbiology, Vol 21, Iss 1, Pp 1-18 (2021) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Miscanthus x giganteus Solid-state fermentation Phanerochaete chrysosporium Microscopy and image processing Spore germination Mycelial growth Microbiology QR1-502 |
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Miscanthus x giganteus Solid-state fermentation Phanerochaete chrysosporium Microscopy and image processing Spore germination Mycelial growth Microbiology QR1-502 Hassan Khalil Estelle Legin Bernard Kurek Patrick Perre Behnam Taidi Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions |
| description |
Abstract Background Solid-state fermentation is a fungal culture technique used to produce compounds and products of industrial interest. The growth behaviour of filamentous fungi on solid media is challenging to study due to the intermixity of the substrate and the growing organism. Several strategies are available to measure indirectly the fungal biomass during the fermentation such as following the biochemical production of mycelium-specific components or microscopic observation. The microscopic observation of the development of the mycelium, on lignocellulosic substrate, has not been reported. In this study, we set up an experimental protocol based on microscopy and image processing through which we investigated the growth pattern of Phanerochaete chrysosporium on different Miscanthus x giganteus biomass fractions. Results Object coalescence, the occupied surface area, and radial expansion of the colony were measured in time. The substrate was sterilized by autoclaving, which could be considered a type of pre-treatment. The fastest growth rate was measured on the unfractionated biomass, followed by the soluble fraction of the biomass, then the residual solid fractions. The growth rate on the different fractions of the substrate was additive, suggesting that both the solid and soluble fractions were used by the fungus. Based on the FTIR analysis, there were differences in composition between the solid and soluble fractions of the substrate, but the main components for growth were always present. We propose using this novel method for measuring the very initial fungal growth by following the variation of the number of objects over time. Once growth is established, the growth can be followed by measurement of the occupied surface by the mycelium. Conclusion Our data showed that the growth was affected from the very beginning by the nature of the substrate. The most extensive colonization of the surface was observed with the unfractionated substrate containing both soluble and solid components. The methodology was practical and may be applied to investigate the growth of other fungi, including the influence of environmental parameters on the fungal growth. |
| format |
article |
| author |
Hassan Khalil Estelle Legin Bernard Kurek Patrick Perre Behnam Taidi |
| author_facet |
Hassan Khalil Estelle Legin Bernard Kurek Patrick Perre Behnam Taidi |
| author_sort |
Hassan Khalil |
| title |
Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions |
| title_short |
Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions |
| title_full |
Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions |
| title_fullStr |
Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions |
| title_full_unstemmed |
Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions |
| title_sort |
morphological growth pattern of phanerochaete chrysosporium cultivated on different miscanthus x giganteus biomass fractions |
| publisher |
BMC |
| publishDate |
2021 |
| url |
https://doaj.org/article/9f1c7590eae34bed85eef52b5146b78a |
| work_keys_str_mv |
AT hassankhalil morphologicalgrowthpatternofphanerochaetechrysosporiumcultivatedondifferentmiscanthusxgiganteusbiomassfractions AT estellelegin morphologicalgrowthpatternofphanerochaetechrysosporiumcultivatedondifferentmiscanthusxgiganteusbiomassfractions AT bernardkurek morphologicalgrowthpatternofphanerochaetechrysosporiumcultivatedondifferentmiscanthusxgiganteusbiomassfractions AT patrickperre morphologicalgrowthpatternofphanerochaetechrysosporiumcultivatedondifferentmiscanthusxgiganteusbiomassfractions AT behnamtaidi morphologicalgrowthpatternofphanerochaetechrysosporiumcultivatedondifferentmiscanthusxgiganteusbiomassfractions |
| _version_ |
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