Core–shell magnetic nanoparticles display synergistic antibacterial effects against Pseudomonas aeruginosa and Staphylococcus aureus when combined with cathelicidin LL-37 or selected ceragenins
Katarzyna Niemirowicz,1 Ewelina Piktel,1 Agnieszka Z Wilczewska,2 Karolina H Markiewicz,2 Bonita Durnaś,3 Marzena Wątek,4 Irena Puszkarz,3 Marta Wróblewska,5,6 Wieslawa Niklińska,7 Paul B Savage,8 Robert Bucki1,3 1Department of Microbiological and Nanobiomedical Engineering, Medical Univ...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2016
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9f1d9cf600ce49c79f8263dcffacd3a1 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9f1d9cf600ce49c79f8263dcffacd3a1 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:9f1d9cf600ce49c79f8263dcffacd3a12021-12-02T01:55:20ZCore–shell magnetic nanoparticles display synergistic antibacterial effects against Pseudomonas aeruginosa and Staphylococcus aureus when combined with cathelicidin LL-37 or selected ceragenins1178-2013https://doaj.org/article/9f1d9cf600ce49c79f8263dcffacd3a12016-10-01T00:00:00Zhttps://www.dovepress.com/corendashshell-magnetic-nanoparticles-display-synergistic-antibacteria-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Katarzyna Niemirowicz,1 Ewelina Piktel,1 Agnieszka Z Wilczewska,2 Karolina H Markiewicz,2 Bonita Durnaś,3 Marzena Wątek,4 Irena Puszkarz,3 Marta Wróblewska,5,6 Wieslawa Niklińska,7 Paul B Savage,8 Robert Bucki1,3 1Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, 2Institute of Chemistry, University of Bialystok, Bialystok, 3Department of Physiology, Pathophysiology and Immunology of Infections, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, 4Holy Cross Oncology Center of Kielce, Kielce, 5Department of Dental Microbiology, Medical University of Warsaw, 6Department of Microbiology, Central Clinical Hospital in Warsaw, Warsaw, 7Department of Histology and Embryology, Medical University of Bialystok, Bialystok, Poland; 8Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA Abstract: Core–shell magnetic nanoparticles (MNPs) are promising candidates in the development of new treatment methods against infections, including those caused by antibiotic-resistant pathogens. In this study, the bactericidal activity of human antibacterial peptide cathelicidin LL-37, synthetic ceragenins CSA-13 and CSA-131, and classical antibiotics vancomycin and colistin, against methicillin-resistant Staphylococcus aureus Xen 30 and Pseudomonas aeruginosa Xen 5, was assessed alone and in combination with core–shell MNPs. Fractional inhibitory concentration index and fractional bactericidal concentration index were determined by microdilution methods. The potential of combined therapy using nanomaterials and selected antibiotics was confirmed using chemiluminescence measurements. Additionally, the ability of tested agents to prevent bacterial biofilm formation was evaluated using crystal violet staining. In most conditions, synergistic or additive effects were observed when combinations of core–shell MNPs with ceragenins or classical antibiotics were used. Our study revealed that a mixture of membrane-active agents such as LL-37 peptide or ceragenin CSA-13 with MNPs potentialized their antibacterial properties and might be considered as a method of delaying and overcoming bacterial drug resistance. Keywords: synergistic activity, antibiotic-resistant bacteria, LL-37 peptide, ceragenins, magnetic nanoparticlesNiemirowicz KPiktel EWilczewska AZMarkiewicz KHDurnaś BWątek MPuszkarz IWróblewska MNiklińska WSavage PBBucki RDove Medical Pressarticlesynergistic activityantibiotic resistant bacteriaLL-37 peptidecerageninsmagnetic nanoparticlesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5443-5455 (2016) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
synergistic activity antibiotic resistant bacteria LL-37 peptide ceragenins magnetic nanoparticles Medicine (General) R5-920 |
| spellingShingle |
synergistic activity antibiotic resistant bacteria LL-37 peptide ceragenins magnetic nanoparticles Medicine (General) R5-920 Niemirowicz K Piktel E Wilczewska AZ Markiewicz KH Durnaś B Wątek M Puszkarz I Wróblewska M Niklińska W Savage PB Bucki R Core–shell magnetic nanoparticles display synergistic antibacterial effects against Pseudomonas aeruginosa and Staphylococcus aureus when combined with cathelicidin LL-37 or selected ceragenins |
| description |
Katarzyna Niemirowicz,1 Ewelina Piktel,1 Agnieszka Z Wilczewska,2 Karolina H Markiewicz,2 Bonita Durnaś,3 Marzena Wątek,4 Irena Puszkarz,3 Marta Wróblewska,5,6 Wieslawa Niklińska,7 Paul B Savage,8 Robert Bucki1,3 1Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, 2Institute of Chemistry, University of Bialystok, Bialystok, 3Department of Physiology, Pathophysiology and Immunology of Infections, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, 4Holy Cross Oncology Center of Kielce, Kielce, 5Department of Dental Microbiology, Medical University of Warsaw, 6Department of Microbiology, Central Clinical Hospital in Warsaw, Warsaw, 7Department of Histology and Embryology, Medical University of Bialystok, Bialystok, Poland; 8Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA Abstract: Core–shell magnetic nanoparticles (MNPs) are promising candidates in the development of new treatment methods against infections, including those caused by antibiotic-resistant pathogens. In this study, the bactericidal activity of human antibacterial peptide cathelicidin LL-37, synthetic ceragenins CSA-13 and CSA-131, and classical antibiotics vancomycin and colistin, against methicillin-resistant Staphylococcus aureus Xen 30 and Pseudomonas aeruginosa Xen 5, was assessed alone and in combination with core–shell MNPs. Fractional inhibitory concentration index and fractional bactericidal concentration index were determined by microdilution methods. The potential of combined therapy using nanomaterials and selected antibiotics was confirmed using chemiluminescence measurements. Additionally, the ability of tested agents to prevent bacterial biofilm formation was evaluated using crystal violet staining. In most conditions, synergistic or additive effects were observed when combinations of core–shell MNPs with ceragenins or classical antibiotics were used. Our study revealed that a mixture of membrane-active agents such as LL-37 peptide or ceragenin CSA-13 with MNPs potentialized their antibacterial properties and might be considered as a method of delaying and overcoming bacterial drug resistance. Keywords: synergistic activity, antibiotic-resistant bacteria, LL-37 peptide, ceragenins, magnetic nanoparticles |
| format |
article |
| author |
Niemirowicz K Piktel E Wilczewska AZ Markiewicz KH Durnaś B Wątek M Puszkarz I Wróblewska M Niklińska W Savage PB Bucki R |
| author_facet |
Niemirowicz K Piktel E Wilczewska AZ Markiewicz KH Durnaś B Wątek M Puszkarz I Wróblewska M Niklińska W Savage PB Bucki R |
| author_sort |
Niemirowicz K |
| title |
Core–shell magnetic nanoparticles display synergistic antibacterial effects against Pseudomonas aeruginosa and Staphylococcus aureus when combined with cathelicidin LL-37 or selected ceragenins |
| title_short |
Core–shell magnetic nanoparticles display synergistic antibacterial effects against Pseudomonas aeruginosa and Staphylococcus aureus when combined with cathelicidin LL-37 or selected ceragenins |
| title_full |
Core–shell magnetic nanoparticles display synergistic antibacterial effects against Pseudomonas aeruginosa and Staphylococcus aureus when combined with cathelicidin LL-37 or selected ceragenins |
| title_fullStr |
Core–shell magnetic nanoparticles display synergistic antibacterial effects against Pseudomonas aeruginosa and Staphylococcus aureus when combined with cathelicidin LL-37 or selected ceragenins |
| title_full_unstemmed |
Core–shell magnetic nanoparticles display synergistic antibacterial effects against Pseudomonas aeruginosa and Staphylococcus aureus when combined with cathelicidin LL-37 or selected ceragenins |
| title_sort |
core–shell magnetic nanoparticles display synergistic antibacterial effects against pseudomonas aeruginosa and staphylococcus aureus when combined with cathelicidin ll-37 or selected ceragenins |
| publisher |
Dove Medical Press |
| publishDate |
2016 |
| url |
https://doaj.org/article/9f1d9cf600ce49c79f8263dcffacd3a1 |
| work_keys_str_mv |
AT niemirowiczk corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins AT piktele corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins AT wilczewskaaz corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins AT markiewiczkh corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins AT durnasb corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins AT watekm corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins AT puszkarzi corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins AT wroblewskam corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins AT niklinskaw corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins AT savagepb corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins AT buckir corendashshellmagneticnanoparticlesdisplaysynergisticantibacterialeffectsagainstpseudomonasaeruginosaandstaphylococcusaureuswhencombinedwithcathelicidinll37orselectedceragenins |
| _version_ |
1718402793119678464 |