An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.

The microbial flora associated with Hyalomma anatolicum ticks was investigated using culture-dependent (CD) and independent (next generation sequencing, NGS) methods. The bacterial profiles of different organs, development stages, sexes, and of host cattle skins were analyzed using the CD method. Th...

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Autores principales:	Nayyereh Choubdar, Fateh Karimian, Mona Koosha, Mohammad Ali Oshaghi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/9f1ee44990ac4ae8bf3e452c510b376e
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spelling	oai:doaj.org-article:9f1ee44990ac4ae8bf3e452c510b376e2021-11-25T06:33:09ZAn integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.1935-27271935-273510.1371/journal.pntd.0009480https://doaj.org/article/9f1ee44990ac4ae8bf3e452c510b376e2021-06-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009480https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735The microbial flora associated with Hyalomma anatolicum ticks was investigated using culture-dependent (CD) and independent (next generation sequencing, NGS) methods. The bacterial profiles of different organs, development stages, sexes, and of host cattle skins were analyzed using the CD method. The egg and female gut microbiota were investigated using NGS. Fourteen distinct bacterial strains were identified using the CD method, of which Bacillus subtilis predominated in eggs, larval guts and in adult female and male guts, suggesting probable transovarial transmission. Bacillus velezensis and B. subtilis were identified in cattle skin and tick samples, suggesting that skin is the origin of tick bacteria. H.anatolicum males harbour lower bacterial diversity and composition than females. The NGS analysis revealed five different bacterial phyla across all samples, Proteobacteria contributing to >95% of the bacteria. In all, 56611sequences were generated representing 6,023 OTUs per female gut and 421 OTUs per egg. Francisellaceae family and Francisella make up the vast majority of the OTUs. Our findings are consistent with interference between Francisella and Rickettsia. The CD method identified bacteria, such B. subtilis that are candidates for vector control intervention approaches such paratransgenesis whereas NGS revealed high Francisella spp. prevalence, indicating that integrated methods are more accurate to characterize microbial community and diversity.Nayyereh ChoubdarFateh KarimianMona KooshaMohammad Ali OshaghiPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009480 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Nayyereh Choubdar Fateh Karimian Mona Koosha Mohammad Ali Oshaghi An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.
description	The microbial flora associated with Hyalomma anatolicum ticks was investigated using culture-dependent (CD) and independent (next generation sequencing, NGS) methods. The bacterial profiles of different organs, development stages, sexes, and of host cattle skins were analyzed using the CD method. The egg and female gut microbiota were investigated using NGS. Fourteen distinct bacterial strains were identified using the CD method, of which Bacillus subtilis predominated in eggs, larval guts and in adult female and male guts, suggesting probable transovarial transmission. Bacillus velezensis and B. subtilis were identified in cattle skin and tick samples, suggesting that skin is the origin of tick bacteria. H.anatolicum males harbour lower bacterial diversity and composition than females. The NGS analysis revealed five different bacterial phyla across all samples, Proteobacteria contributing to >95% of the bacteria. In all, 56611sequences were generated representing 6,023 OTUs per female gut and 421 OTUs per egg. Francisellaceae family and Francisella make up the vast majority of the OTUs. Our findings are consistent with interference between Francisella and Rickettsia. The CD method identified bacteria, such B. subtilis that are candidates for vector control intervention approaches such paratransgenesis whereas NGS revealed high Francisella spp. prevalence, indicating that integrated methods are more accurate to characterize microbial community and diversity.
format	article
author	Nayyereh Choubdar Fateh Karimian Mona Koosha Mohammad Ali Oshaghi
author_facet	Nayyereh Choubdar Fateh Karimian Mona Koosha Mohammad Ali Oshaghi
author_sort	Nayyereh Choubdar
title	An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.
title_short	An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.
title_full	An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.
title_fullStr	An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.
title_full_unstemmed	An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.
title_sort	integrated overview of the bacterial flora composition of hyalomma anatolicum, the main vector of cchf.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9f1ee44990ac4ae8bf3e452c510b376e
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An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.

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