Role of CAP350 in centriolar tubule stability and centriole assembly.

<h4>Background</h4>Centrioles are microtubule-based cylindrical structures composed of nine triplet tubules and are required for the formation of the centrosome, flagella and cilia. Despite theirs importance, centriole biogenesis is poorly understood. Centrosome duplication is initiated...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Mikael Le Clech
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f236b4526474d9d8d9aba443324084b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9f236b4526474d9d8d9aba443324084b
record_format	dspace
spelling	oai:doaj.org-article:9f236b4526474d9d8d9aba443324084b2021-11-25T06:18:19ZRole of CAP350 in centriolar tubule stability and centriole assembly.1932-620310.1371/journal.pone.0003855https://doaj.org/article/9f236b4526474d9d8d9aba443324084b2008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19052644/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Centrioles are microtubule-based cylindrical structures composed of nine triplet tubules and are required for the formation of the centrosome, flagella and cilia. Despite theirs importance, centriole biogenesis is poorly understood. Centrosome duplication is initiated at the G1/S transition by the sequential recruitment of a set of conserved proteins under the control of the kinase Plk4. Subsequently, the procentriole is assembled by the polymerization of centriolar tubules via an unknown mechanism involving several tubulin paralogs.<h4>Methodology/principal findings</h4>Here, we developed a cellular assay to study centrosome duplication and procentriole stability based on its sensitivity to the microtubule-depolymerizing drug nocodazole. By using RNA interference experiments, we show that the stability of growing procentrioles is regulated by the microtubule-stabilizing protein CAP350, independently of hSAS-6 and CPAP which initiate procentriole growth. Furthermore, our analysis reveals the critical role of centriolar tubule stability for an efficient procentriole growth.<h4>Conclusions/significance</h4>CAP350 belongs to a new class of proteins which associate and stabilize centriolar tubules to control centriole duplication.Mikael Le ClechPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 12, p e3855 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mikael Le Clech Role of CAP350 in centriolar tubule stability and centriole assembly.
description	<h4>Background</h4>Centrioles are microtubule-based cylindrical structures composed of nine triplet tubules and are required for the formation of the centrosome, flagella and cilia. Despite theirs importance, centriole biogenesis is poorly understood. Centrosome duplication is initiated at the G1/S transition by the sequential recruitment of a set of conserved proteins under the control of the kinase Plk4. Subsequently, the procentriole is assembled by the polymerization of centriolar tubules via an unknown mechanism involving several tubulin paralogs.<h4>Methodology/principal findings</h4>Here, we developed a cellular assay to study centrosome duplication and procentriole stability based on its sensitivity to the microtubule-depolymerizing drug nocodazole. By using RNA interference experiments, we show that the stability of growing procentrioles is regulated by the microtubule-stabilizing protein CAP350, independently of hSAS-6 and CPAP which initiate procentriole growth. Furthermore, our analysis reveals the critical role of centriolar tubule stability for an efficient procentriole growth.<h4>Conclusions/significance</h4>CAP350 belongs to a new class of proteins which associate and stabilize centriolar tubules to control centriole duplication.
format	article
author	Mikael Le Clech
author_facet	Mikael Le Clech
author_sort	Mikael Le Clech
title	Role of CAP350 in centriolar tubule stability and centriole assembly.
title_short	Role of CAP350 in centriolar tubule stability and centriole assembly.
title_full	Role of CAP350 in centriolar tubule stability and centriole assembly.
title_fullStr	Role of CAP350 in centriolar tubule stability and centriole assembly.
title_full_unstemmed	Role of CAP350 in centriolar tubule stability and centriole assembly.
title_sort	role of cap350 in centriolar tubule stability and centriole assembly.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/9f236b4526474d9d8d9aba443324084b
work_keys_str_mv	AT mikaelleclech roleofcap350incentriolartubulestabilityandcentrioleassembly
_version_	1718413940574126080

Role of CAP350 in centriolar tubule stability and centriole assembly.

Ejemplares similares