MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells

Cancer cells show significant dysregulation of genes expression, which may favor their survival in the tumor environment. In this study, the cellular vault’s components MVP (major vault protein), TEP1 (telomerase-associated protein 1) and vPARP (vault poly(ADP-ribose) polymerase) were transiently or...

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Autores principales:	Paulina Pietras, Marta Leśniczak-Staszak, Aldona Kasprzak, Małgorzata Andrzejewska, Karol Jopek, Mateusz Sowiński, Marcin Rucinski, Shawn M. Lyons, Pavel Ivanov, Witold Szaflarski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	MVP TEP1 vPARP vaults colorectal cancer metastasis Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/9f23c0ccaaaf4f938f18e46d5eae16ca
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spelling	oai:doaj.org-article:9f23c0ccaaaf4f938f18e46d5eae16ca2021-11-25T17:53:33ZMVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells10.3390/ijms2222121211422-00671661-6596https://doaj.org/article/9f23c0ccaaaf4f938f18e46d5eae16ca2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12121https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cancer cells show significant dysregulation of genes expression, which may favor their survival in the tumor environment. In this study, the cellular vault’s components MVP (major vault protein), TEP1 (telomerase-associated protein 1) and vPARP (vault poly(ADP-ribose) polymerase) were transiently or completely inhibited in U2OS cells (human bone osteosarcoma epithelial cells) to evaluate their impact on the cell proliferative and migratory capacity as well as on the development of their resistance to the drug vinorelbine. Comparative analysis of MVP protein expression level in normal colon tissue, primary colorectal tumor, and metastasis showed that the expression of this protein does not increase significantly in the primary tumor, but its expression increases in metastatic cells. Further comparative molecular analysis using the whole transcriptome microarrays for MVP-positive and MVP-negative cells showed that MVP is involved in regulating proliferation and migration of cancer cells. MVP may facilitate metastasis of colon cancer due to its impact on cell migration. Moreover, two vault proteins, MVP and TEP1, contribute the resistance to vinorelbine, while vPARP does not.Paulina PietrasMarta Leśniczak-StaszakAldona KasprzakMałgorzata AndrzejewskaKarol JopekMateusz SowińskiMarcin RucinskiShawn M. LyonsPavel IvanovWitold SzaflarskiMDPI AGarticleMVPTEP1vPARPvaultscolorectal cancermetastasisBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12121, p 12121 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MVP TEP1 vPARP vaults colorectal cancer metastasis Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	MVP TEP1 vPARP vaults colorectal cancer metastasis Biology (General) QH301-705.5 Chemistry QD1-999 Paulina Pietras Marta Leśniczak-Staszak Aldona Kasprzak Małgorzata Andrzejewska Karol Jopek Mateusz Sowiński Marcin Rucinski Shawn M. Lyons Pavel Ivanov Witold Szaflarski MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells
description	Cancer cells show significant dysregulation of genes expression, which may favor their survival in the tumor environment. In this study, the cellular vault’s components MVP (major vault protein), TEP1 (telomerase-associated protein 1) and vPARP (vault poly(ADP-ribose) polymerase) were transiently or completely inhibited in U2OS cells (human bone osteosarcoma epithelial cells) to evaluate their impact on the cell proliferative and migratory capacity as well as on the development of their resistance to the drug vinorelbine. Comparative analysis of MVP protein expression level in normal colon tissue, primary colorectal tumor, and metastasis showed that the expression of this protein does not increase significantly in the primary tumor, but its expression increases in metastatic cells. Further comparative molecular analysis using the whole transcriptome microarrays for MVP-positive and MVP-negative cells showed that MVP is involved in regulating proliferation and migration of cancer cells. MVP may facilitate metastasis of colon cancer due to its impact on cell migration. Moreover, two vault proteins, MVP and TEP1, contribute the resistance to vinorelbine, while vPARP does not.
format	article
author	Paulina Pietras Marta Leśniczak-Staszak Aldona Kasprzak Małgorzata Andrzejewska Karol Jopek Mateusz Sowiński Marcin Rucinski Shawn M. Lyons Pavel Ivanov Witold Szaflarski
author_facet	Paulina Pietras Marta Leśniczak-Staszak Aldona Kasprzak Małgorzata Andrzejewska Karol Jopek Mateusz Sowiński Marcin Rucinski Shawn M. Lyons Pavel Ivanov Witold Szaflarski
author_sort	Paulina Pietras
title	MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells
title_short	MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells
title_full	MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells
title_fullStr	MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells
title_full_unstemmed	MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells
title_sort	mvp expression facilitates tumor cell proliferation and migration supporting the metastasis of colorectal cancer cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9f23c0ccaaaf4f938f18e46d5eae16ca
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MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells

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