Unravelling the involvement of cilevirus p32 protein in the viral transport

Abstract Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a det...

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Autores principales:	Mikhail Oliveira Leastro, Juliana Freitas-Astúa, Elliot Watanabe Kitajima, Vicente Pallás, Jesús A. Sánchez-Navarro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f24a84a05674a95a986c3df495690e5
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spelling	oai:doaj.org-article:9f24a84a05674a95a986c3df495690e52021-12-02T14:06:31ZUnravelling the involvement of cilevirus p32 protein in the viral transport10.1038/s41598-021-82453-42045-2322https://doaj.org/article/9f24a84a05674a95a986c3df495690e52021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82453-4https://doaj.org/toc/2045-2322Abstract Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread.Mikhail Oliveira LeastroJuliana Freitas-AstúaElliot Watanabe KitajimaVicente PallásJesús A. Sánchez-NavarroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mikhail Oliveira Leastro Juliana Freitas-Astúa Elliot Watanabe Kitajima Vicente Pallás Jesús A. Sánchez-Navarro Unravelling the involvement of cilevirus p32 protein in the viral transport
description	Abstract Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread.
format	article
author	Mikhail Oliveira Leastro Juliana Freitas-Astúa Elliot Watanabe Kitajima Vicente Pallás Jesús A. Sánchez-Navarro
author_facet	Mikhail Oliveira Leastro Juliana Freitas-Astúa Elliot Watanabe Kitajima Vicente Pallás Jesús A. Sánchez-Navarro
author_sort	Mikhail Oliveira Leastro
title	Unravelling the involvement of cilevirus p32 protein in the viral transport
title_short	Unravelling the involvement of cilevirus p32 protein in the viral transport
title_full	Unravelling the involvement of cilevirus p32 protein in the viral transport
title_fullStr	Unravelling the involvement of cilevirus p32 protein in the viral transport
title_full_unstemmed	Unravelling the involvement of cilevirus p32 protein in the viral transport
title_sort	unravelling the involvement of cilevirus p32 protein in the viral transport
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9f24a84a05674a95a986c3df495690e5
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Unravelling the involvement of cilevirus p32 protein in the viral transport

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