Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures

Abstract Our skin provides a physical barrier to separate the internal part of our body from the environment. Maintenance of complex barrier functions is achieved through anatomical structures in the skin, the stratified squamous epithelium specialized junctional organelles, called tight junctions (...

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Autores principales:	Beáta Szilvia Bolla, Lilla Erdei, Edit Urbán, Katalin Burián, Lajos Kemény, Kornélia Szabó
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f25c93d653e4651b1df3b79a991d6b9
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spelling	oai:doaj.org-article:9f25c93d653e4651b1df3b79a991d6b92021-12-02T16:06:39ZCutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures10.1038/s41598-020-69677-62045-2322https://doaj.org/article/9f25c93d653e4651b1df3b79a991d6b92020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-69677-6https://doaj.org/toc/2045-2322Abstract Our skin provides a physical barrier to separate the internal part of our body from the environment. Maintenance of complex barrier functions is achieved through anatomical structures in the skin, the stratified squamous epithelium specialized junctional organelles, called tight junctions (TJs). Several members of our microbial communities are known to affect the differentiation state and function of the colonized organ. Whether and how interactions between skin cells and cutaneous microbes, including Cutibacterium acnes (C. acnes), modify the structure and/or function of our skin is currently only partly understood. Thus, in our studies, we investigated whether C. acnes may affect the epidermal barrier using in vitro model systems. Real-time cellular analysis showed that depending on the keratinocyte differentiation state, the applied C. acnes strains and their dose, the measured impedance values change, together with the expression of selected TJ proteins. These may reflect barrier alterations, which can be partially restored upon antibiotic–antimycotic treatment. Our findings suggest that C. acnes can actively modify the barrier properties of cultured keratinocytes, possibly through alteration of tight cell-to-cell contacts. Similar events may play important roles in our skin, in the maintenance of cutaneous homeostasis.Beáta Szilvia BollaLilla ErdeiEdit UrbánKatalin BuriánLajos KeményKornélia SzabóNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Beáta Szilvia Bolla Lilla Erdei Edit Urbán Katalin Burián Lajos Kemény Kornélia Szabó Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures
description	Abstract Our skin provides a physical barrier to separate the internal part of our body from the environment. Maintenance of complex barrier functions is achieved through anatomical structures in the skin, the stratified squamous epithelium specialized junctional organelles, called tight junctions (TJs). Several members of our microbial communities are known to affect the differentiation state and function of the colonized organ. Whether and how interactions between skin cells and cutaneous microbes, including Cutibacterium acnes (C. acnes), modify the structure and/or function of our skin is currently only partly understood. Thus, in our studies, we investigated whether C. acnes may affect the epidermal barrier using in vitro model systems. Real-time cellular analysis showed that depending on the keratinocyte differentiation state, the applied C. acnes strains and their dose, the measured impedance values change, together with the expression of selected TJ proteins. These may reflect barrier alterations, which can be partially restored upon antibiotic–antimycotic treatment. Our findings suggest that C. acnes can actively modify the barrier properties of cultured keratinocytes, possibly through alteration of tight cell-to-cell contacts. Similar events may play important roles in our skin, in the maintenance of cutaneous homeostasis.
format	article
author	Beáta Szilvia Bolla Lilla Erdei Edit Urbán Katalin Burián Lajos Kemény Kornélia Szabó
author_facet	Beáta Szilvia Bolla Lilla Erdei Edit Urbán Katalin Burián Lajos Kemény Kornélia Szabó
author_sort	Beáta Szilvia Bolla
title	Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures
title_short	Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures
title_full	Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures
title_fullStr	Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures
title_full_unstemmed	Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures
title_sort	cutibacterium acnes regulates the epidermal barrier properties of hpv-ker human immortalized keratinocyte cultures
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9f25c93d653e4651b1df3b79a991d6b9
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Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures

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