Quantitative Proteomics and Transcriptomics Reveals Differences in Proteins During Anthers Development in Oryza longistaminata

Oryza longistaminata is an African wild rice species that possesses special traits for breeding applications. Self-incompatibility is the main cause of sterility in O. longistaminata, but here we demonstrated that its pollen vitality are normal. Lipid and carbohydrate metabolism were active througho...

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Autores principales:	Yue Sun, Xin Wang, Zhongkai Chen, Lu Qin, Bai Li, Linjuan Ouyang, Xiaosong Peng, Haohua He
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Oryza longistaminata pollen viability proteome fatty acid glyoxylate cycle gluconeogenesis Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/9f2c97b120c84d948e7af98efad6d423
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spelling	oai:doaj.org-article:9f2c97b120c84d948e7af98efad6d4232021-11-19T07:36:30ZQuantitative Proteomics and Transcriptomics Reveals Differences in Proteins During Anthers Development in Oryza longistaminata1664-462X10.3389/fpls.2021.744792https://doaj.org/article/9f2c97b120c84d948e7af98efad6d4232021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.744792/fullhttps://doaj.org/toc/1664-462XOryza longistaminata is an African wild rice species that possesses special traits for breeding applications. Self-incompatibility is the main cause of sterility in O. longistaminata, but here we demonstrated that its pollen vitality are normal. Lipid and carbohydrate metabolism were active throughout pollen development. In this study, we used I2-KI staining and TTC staining to investigate pollen viability. Aniline-blue-stained semithin sections were used to investigate important stages of pollen development. Tandem mass tags (TMT)-based quantitative analysis was used to investigate the profiles of proteins related to lipid and carbohydrate metabolism in 4-, 6-, and 8.5-mm O. longistaminata spikelets before flowering. Pollen was found to germinate normally in vitro and in vivo. We documented cytological changes throughout important stages of anther development, including changes in reproductive cells as they formed mature pollen grains through meiosis and mitosis. A total of 31,987 RNA transcripts and 8,753 proteins were identified, and 6,842 of the proteins could be quantified. RNA-seq and proteome association analysis indicated that fatty acids were converted to sucrose after the 6-mm spikelet stage, based on the abundance of most key enzymes of the glyoxylate cycle and gluconeogenesis. The abundance of proteins involved in pollen energy metabolism was further confirmed by combining quantitative real-time PCR with parallel reaction monitoring (PRM) analyses. In conclusion, our study provides novel insights into the pollen viability of O. longistaminata at the proteome level, which can be used to improve the efficiency of male parent pollination in hybrid rice breeding applications.Yue SunXin WangZhongkai ChenLu QinBai LiLinjuan OuyangXiaosong PengHaohua HeFrontiers Media S.A.articleOryza longistaminatapollen viabilityproteomefatty acidglyoxylate cyclegluconeogenesisPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Oryza longistaminata pollen viability proteome fatty acid glyoxylate cycle gluconeogenesis Plant culture SB1-1110
spellingShingle	Oryza longistaminata pollen viability proteome fatty acid glyoxylate cycle gluconeogenesis Plant culture SB1-1110 Yue Sun Xin Wang Zhongkai Chen Lu Qin Bai Li Linjuan Ouyang Xiaosong Peng Haohua He Quantitative Proteomics and Transcriptomics Reveals Differences in Proteins During Anthers Development in Oryza longistaminata
description	Oryza longistaminata is an African wild rice species that possesses special traits for breeding applications. Self-incompatibility is the main cause of sterility in O. longistaminata, but here we demonstrated that its pollen vitality are normal. Lipid and carbohydrate metabolism were active throughout pollen development. In this study, we used I2-KI staining and TTC staining to investigate pollen viability. Aniline-blue-stained semithin sections were used to investigate important stages of pollen development. Tandem mass tags (TMT)-based quantitative analysis was used to investigate the profiles of proteins related to lipid and carbohydrate metabolism in 4-, 6-, and 8.5-mm O. longistaminata spikelets before flowering. Pollen was found to germinate normally in vitro and in vivo. We documented cytological changes throughout important stages of anther development, including changes in reproductive cells as they formed mature pollen grains through meiosis and mitosis. A total of 31,987 RNA transcripts and 8,753 proteins were identified, and 6,842 of the proteins could be quantified. RNA-seq and proteome association analysis indicated that fatty acids were converted to sucrose after the 6-mm spikelet stage, based on the abundance of most key enzymes of the glyoxylate cycle and gluconeogenesis. The abundance of proteins involved in pollen energy metabolism was further confirmed by combining quantitative real-time PCR with parallel reaction monitoring (PRM) analyses. In conclusion, our study provides novel insights into the pollen viability of O. longistaminata at the proteome level, which can be used to improve the efficiency of male parent pollination in hybrid rice breeding applications.
format	article
author	Yue Sun Xin Wang Zhongkai Chen Lu Qin Bai Li Linjuan Ouyang Xiaosong Peng Haohua He
author_facet	Yue Sun Xin Wang Zhongkai Chen Lu Qin Bai Li Linjuan Ouyang Xiaosong Peng Haohua He
author_sort	Yue Sun
title	Quantitative Proteomics and Transcriptomics Reveals Differences in Proteins During Anthers Development in Oryza longistaminata
title_short	Quantitative Proteomics and Transcriptomics Reveals Differences in Proteins During Anthers Development in Oryza longistaminata
title_full	Quantitative Proteomics and Transcriptomics Reveals Differences in Proteins During Anthers Development in Oryza longistaminata
title_fullStr	Quantitative Proteomics and Transcriptomics Reveals Differences in Proteins During Anthers Development in Oryza longistaminata
title_full_unstemmed	Quantitative Proteomics and Transcriptomics Reveals Differences in Proteins During Anthers Development in Oryza longistaminata
title_sort	quantitative proteomics and transcriptomics reveals differences in proteins during anthers development in oryza longistaminata
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9f2c97b120c84d948e7af98efad6d423
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Quantitative Proteomics and Transcriptomics Reveals Differences in Proteins During Anthers Development in Oryza longistaminata

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