Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB

Acute lung injury (ALI) is associated with a high mortality due to inflammatory cell infiltration and lung edema. The development of ALI commonly involves the activation of NF-κB. Since bergamottin is a natural furanocoumarin showing the ability to inhibit the activation of NF-κB, in this study we a...

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Autores principales:	Ning An, Tao Yang, Xiao-Xia Zhang, Mei-xia Xu
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9f326470c0764035ad44ffbe8d667b65
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spelling	oai:doaj.org-article:9f326470c0764035ad44ffbe8d667b652021-12-02T07:35:09ZBergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB1753-42591753-426710.1177/17534259211062553https://doaj.org/article/9f326470c0764035ad44ffbe8d667b652021-10-01T00:00:00Zhttps://doi.org/10.1177/17534259211062553https://doaj.org/toc/1753-4259https://doaj.org/toc/1753-4267Acute lung injury (ALI) is associated with a high mortality due to inflammatory cell infiltration and lung edema. The development of ALI commonly involves the activation of NF-κB. Since bergamottin is a natural furanocoumarin showing the ability to inhibit the activation of NF-κB, in this study we aimed to determine the effect of bergamottin on ALI. RAW264.7 mouse macrophages were pre-treated with bergamottin and then stimulated with LPS. Macrophage inflammatory responses were examined. Bergamottin (50 mg/kg body mass) was intraperitoneally administrated to mice 12 h before injection of LPS, and the effect of bergamottin on LPS-induced ALI was evaluated. Our results showed that LPS exposure led to increased production of TNF-α, IL-6, and monocyte chemoattractant protein-1 (MCP-1), which was impaired by bergamottin pre-treatment. In vivo studies confirmed that bergamottin pre-treatment suppressed LPS-induced lung inflammation and edema and reduced the levels of pro-inflammatory cytokines in lung tissues and bronchoalveolar lavage fluids. Mechanistically, bergamottin blocked LPS-induced activation of NF-κB signaling in lung tissues. Additionally, bergamottin treatment reduced NF-κB p65 protein acetylation, which was coupled with induction of SIRT1 expression. In conclusion, our results reveal the anti-inflammatory property of bergamottin in preventing ALI. Induction of SIRT1 and inhibition of NF-κB underlies the anti-inflammatory activity of bergamottin.Ning AnTao YangXiao-Xia ZhangMei-xia XuSAGE PublishingarticleImmunologic diseases. AllergyRC581-607ENInnate Immunity, Vol 27 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607
spellingShingle	Immunologic diseases. Allergy RC581-607 Ning An Tao Yang Xiao-Xia Zhang Mei-xia Xu Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB
description	Acute lung injury (ALI) is associated with a high mortality due to inflammatory cell infiltration and lung edema. The development of ALI commonly involves the activation of NF-κB. Since bergamottin is a natural furanocoumarin showing the ability to inhibit the activation of NF-κB, in this study we aimed to determine the effect of bergamottin on ALI. RAW264.7 mouse macrophages were pre-treated with bergamottin and then stimulated with LPS. Macrophage inflammatory responses were examined. Bergamottin (50 mg/kg body mass) was intraperitoneally administrated to mice 12 h before injection of LPS, and the effect of bergamottin on LPS-induced ALI was evaluated. Our results showed that LPS exposure led to increased production of TNF-α, IL-6, and monocyte chemoattractant protein-1 (MCP-1), which was impaired by bergamottin pre-treatment. In vivo studies confirmed that bergamottin pre-treatment suppressed LPS-induced lung inflammation and edema and reduced the levels of pro-inflammatory cytokines in lung tissues and bronchoalveolar lavage fluids. Mechanistically, bergamottin blocked LPS-induced activation of NF-κB signaling in lung tissues. Additionally, bergamottin treatment reduced NF-κB p65 protein acetylation, which was coupled with induction of SIRT1 expression. In conclusion, our results reveal the anti-inflammatory property of bergamottin in preventing ALI. Induction of SIRT1 and inhibition of NF-κB underlies the anti-inflammatory activity of bergamottin.
format	article
author	Ning An Tao Yang Xiao-Xia Zhang Mei-xia Xu
author_facet	Ning An Tao Yang Xiao-Xia Zhang Mei-xia Xu
author_sort	Ning An
title	Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB
title_short	Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB
title_full	Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB
title_fullStr	Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB
title_full_unstemmed	Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB
title_sort	bergamottin alleviates lps-induced acute lung injury by inducing sirt1 and suppressing nf-κb
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/9f326470c0764035ad44ffbe8d667b65
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Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB

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