F-actin-dependent regulation of NESH dynamics in rat hippocampal neurons.

Synaptic plasticity is an important feature of neurons essential for learning and memory. Postsynaptic organization and composition are dynamically remodeled in response to diverse synaptic inputs during synaptic plasticity. During this process, the dynamics and localization of postsynaptic proteins...

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Autores principales:	Jeomil Bae, Bong Hwan Sung, In Ha Cho, Woo Keun Song
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f347a4e2b304a2185a22709dc4e0c9d
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spelling	oai:doaj.org-article:9f347a4e2b304a2185a22709dc4e0c9d2021-11-18T07:23:13ZF-actin-dependent regulation of NESH dynamics in rat hippocampal neurons.1932-620310.1371/journal.pone.0034514https://doaj.org/article/9f347a4e2b304a2185a22709dc4e0c9d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22496823/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Synaptic plasticity is an important feature of neurons essential for learning and memory. Postsynaptic organization and composition are dynamically remodeled in response to diverse synaptic inputs during synaptic plasticity. During this process, the dynamics and localization of postsynaptic proteins are also precisely regulated. NESH/Abi-3 is a member of the Abl interactor (Abi) protein family. Overexpression of NESH is associated with reduced cell motility and tumor metastasis. Strong evidence of a close relationship between NESH and the actin cytoskeleton has been documented. Although earlier studies have shown that NESH is prominently expressed in the brain, its function and characteristics are yet to be established. Data from the present investigation suggest that synaptic localization of NESH in hippocampal neurons is regulated in an F-actin-dependent manner. The dynamic fraction of NESH in the dendritic spine was analyzed using FRAP (fluorescence recovery after photobleaching). Interestingly, F-actin stabilization and disruption significantly affected the mobile fraction of NESH, possibly through altered interactions of NESH with the F-actin. In addition, NESH was synaptically targeted from the dendritic shaft to spine after induction of chemical LTP (long-term potentiation) and the translocation was dependent on F-actin. Our data collectively support the significance of the F-actin cytoskeleton in synaptic targeting of NESH as well as its dynamics.Jeomil BaeBong Hwan SungIn Ha ChoWoo Keun SongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34514 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeomil Bae Bong Hwan Sung In Ha Cho Woo Keun Song F-actin-dependent regulation of NESH dynamics in rat hippocampal neurons.
description	Synaptic plasticity is an important feature of neurons essential for learning and memory. Postsynaptic organization and composition are dynamically remodeled in response to diverse synaptic inputs during synaptic plasticity. During this process, the dynamics and localization of postsynaptic proteins are also precisely regulated. NESH/Abi-3 is a member of the Abl interactor (Abi) protein family. Overexpression of NESH is associated with reduced cell motility and tumor metastasis. Strong evidence of a close relationship between NESH and the actin cytoskeleton has been documented. Although earlier studies have shown that NESH is prominently expressed in the brain, its function and characteristics are yet to be established. Data from the present investigation suggest that synaptic localization of NESH in hippocampal neurons is regulated in an F-actin-dependent manner. The dynamic fraction of NESH in the dendritic spine was analyzed using FRAP (fluorescence recovery after photobleaching). Interestingly, F-actin stabilization and disruption significantly affected the mobile fraction of NESH, possibly through altered interactions of NESH with the F-actin. In addition, NESH was synaptically targeted from the dendritic shaft to spine after induction of chemical LTP (long-term potentiation) and the translocation was dependent on F-actin. Our data collectively support the significance of the F-actin cytoskeleton in synaptic targeting of NESH as well as its dynamics.
format	article
author	Jeomil Bae Bong Hwan Sung In Ha Cho Woo Keun Song
author_facet	Jeomil Bae Bong Hwan Sung In Ha Cho Woo Keun Song
author_sort	Jeomil Bae
title	F-actin-dependent regulation of NESH dynamics in rat hippocampal neurons.
title_short	F-actin-dependent regulation of NESH dynamics in rat hippocampal neurons.
title_full	F-actin-dependent regulation of NESH dynamics in rat hippocampal neurons.
title_fullStr	F-actin-dependent regulation of NESH dynamics in rat hippocampal neurons.
title_full_unstemmed	F-actin-dependent regulation of NESH dynamics in rat hippocampal neurons.
title_sort	f-actin-dependent regulation of nesh dynamics in rat hippocampal neurons.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9f347a4e2b304a2185a22709dc4e0c9d
work_keys_str_mv	AT jeomilbae factindependentregulationofneshdynamicsinrathippocampalneurons AT bonghwansung factindependentregulationofneshdynamicsinrathippocampalneurons AT inhacho factindependentregulationofneshdynamicsinrathippocampalneurons AT wookeunsong factindependentregulationofneshdynamicsinrathippocampalneurons
_version_	1718423551378194432

F-actin-dependent regulation of NESH dynamics in rat hippocampal neurons.

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