Reconstitution of ovarian function following transplantation of primordial germ cells

Abstract Ovarian aging occurs earlier than somatic aging. We tested the hypothesis that ovarian functions could be artificially reconstructed by transplantation of primordial germ cells (PGCs). We compared various methods for transplantation of PGCs aggregated with gonadal somatic cells and showed t...

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Autores principales:	Ming Zeng, Xiaoyan Sheng, David L. Keefe, Lin Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f4b8a6ae8104b2ca25b43794d5be889
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spelling	oai:doaj.org-article:9f4b8a6ae8104b2ca25b43794d5be8892021-12-02T12:31:51ZReconstitution of ovarian function following transplantation of primordial germ cells10.1038/s41598-017-01648-w2045-2322https://doaj.org/article/9f4b8a6ae8104b2ca25b43794d5be8892017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01648-whttps://doaj.org/toc/2045-2322Abstract Ovarian aging occurs earlier than somatic aging. We tested the hypothesis that ovarian functions could be artificially reconstructed by transplantation of primordial germ cells (PGCs). We compared various methods for transplantation of PGCs aggregated with gonadal somatic cells and showed that reconstituted ovaries exhibited folliculogenesis after transplantation of PGCs-aggregates into either kidney capsule or ovarian bursa. Neo-oogenesis occurred early after transplantation, as evidenced by the presence of prophase I meiocytes displaying homologous pairing. Moreover, endocrine function was recovered in ovariectomized recipients, including elevated levels of AMH and estradiol. Interestingly, folliculogenesis in the reconstituted ovaries failed to sustain past four weeks. Regardless of transplantation method, follicles diminished after 45 days, accompanied by increased apoptosis, and were undetectable after two months. Meanwhile, no replicative PGCs or prophase I meiocytes could be found. Together, transplantation of PGCs can effectively reconstitute ovarian functions but for limited time. These data suggest that PGCs do not undergo self-renewal but rapidly enter meiosis following transplantation. Global activation of primordial follicles in artificial ovaries can result in further rapid loss of germ cells. Methods for maintaining self-renewal and expansion in vivo of PGCs and controlling follicle activation will be essential for continuing maintenance of the functional reconstructed ovaries.Ming ZengXiaoyan ShengDavid L. KeefeLin LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ming Zeng Xiaoyan Sheng David L. Keefe Lin Liu Reconstitution of ovarian function following transplantation of primordial germ cells
description	Abstract Ovarian aging occurs earlier than somatic aging. We tested the hypothesis that ovarian functions could be artificially reconstructed by transplantation of primordial germ cells (PGCs). We compared various methods for transplantation of PGCs aggregated with gonadal somatic cells and showed that reconstituted ovaries exhibited folliculogenesis after transplantation of PGCs-aggregates into either kidney capsule or ovarian bursa. Neo-oogenesis occurred early after transplantation, as evidenced by the presence of prophase I meiocytes displaying homologous pairing. Moreover, endocrine function was recovered in ovariectomized recipients, including elevated levels of AMH and estradiol. Interestingly, folliculogenesis in the reconstituted ovaries failed to sustain past four weeks. Regardless of transplantation method, follicles diminished after 45 days, accompanied by increased apoptosis, and were undetectable after two months. Meanwhile, no replicative PGCs or prophase I meiocytes could be found. Together, transplantation of PGCs can effectively reconstitute ovarian functions but for limited time. These data suggest that PGCs do not undergo self-renewal but rapidly enter meiosis following transplantation. Global activation of primordial follicles in artificial ovaries can result in further rapid loss of germ cells. Methods for maintaining self-renewal and expansion in vivo of PGCs and controlling follicle activation will be essential for continuing maintenance of the functional reconstructed ovaries.
format	article
author	Ming Zeng Xiaoyan Sheng David L. Keefe Lin Liu
author_facet	Ming Zeng Xiaoyan Sheng David L. Keefe Lin Liu
author_sort	Ming Zeng
title	Reconstitution of ovarian function following transplantation of primordial germ cells
title_short	Reconstitution of ovarian function following transplantation of primordial germ cells
title_full	Reconstitution of ovarian function following transplantation of primordial germ cells
title_fullStr	Reconstitution of ovarian function following transplantation of primordial germ cells
title_full_unstemmed	Reconstitution of ovarian function following transplantation of primordial germ cells
title_sort	reconstitution of ovarian function following transplantation of primordial germ cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9f4b8a6ae8104b2ca25b43794d5be889
work_keys_str_mv	AT mingzeng reconstitutionofovarianfunctionfollowingtransplantationofprimordialgermcells AT xiaoyansheng reconstitutionofovarianfunctionfollowingtransplantationofprimordialgermcells AT davidlkeefe reconstitutionofovarianfunctionfollowingtransplantationofprimordialgermcells AT linliu reconstitutionofovarianfunctionfollowingtransplantationofprimordialgermcells
_version_	1718394231848960000

Reconstitution of ovarian function following transplantation of primordial germ cells

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