GATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance

Background: Urothelial carcinomas [UCs] can be diagnostically challenging, particularly transurethral bladder tumors resection biopsies with limited material. Immunohistochemistry is a valuable instrument for diagnosis when morphology alone is inadequate. GATA-binding protein 3 [GATA3] and cytokerat...

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Autores principales:	Marwa Abdel Moneim El Kholy, Mohamed Ali, Ahmed Aboelsaad, Ahmed Abdel Gawad
Formato:	article
Lenguaje:	EN
Publicado:	Al-Azhar University, Faculty of Medicine (Damietta) 2021
Materias:	urothelial carcinoma transurethral bladder tumor resection muscle-invasive bladder cancers gata‑3, ck 5/6 immunohistochemical Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9f4b954f71ba44779a551d8312ccf314
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spelling	oai:doaj.org-article:9f4b954f71ba44779a551d8312ccf3142021-12-02T15:54:52ZGATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance2636-41742682-378010.21608/ijma.2021.67390.1280https://doaj.org/article/9f4b954f71ba44779a551d8312ccf3142021-04-01T00:00:00Zhttps://ijma.journals.ekb.eg/article_165417_a33d1777fe0b60065d4ec9a610119e8b.pdfhttps://doaj.org/toc/2636-4174https://doaj.org/toc/2682-3780Background: Urothelial carcinomas [UCs] can be diagnostically challenging, particularly transurethral bladder tumors resection biopsies with limited material. Immunohistochemistry is a valuable instrument for diagnosis when morphology alone is inadequate. GATA-binding protein 3 [GATA3] and cytokeratin 5/6 [CK 5/6] contributes to the growth of UC. However, their correlative expression in UC and prognostic value has not been sufficiently investigated. The aim of the work: The current study aimed to evaluate the utility of GATA3 expression in UC and correlate that with CK 5/6 expression to verify different subtypes of UC and assess their prognostic significance. Methodology: Here, we immunohistochemically stained GATA3 and CK 5/6 in 90 UCs samples by transurethral bladder tumor resection in a retrospective study, between May 2018 and February 2020. All were histopathologically evaluated and immunohistochemically stained for GATA3 and CK 5/6, and then correlated them with the clinicopathological parameters to investigate their clinical significance. Results: GATA3 expression was seen in 76 patients [84.44 %]. There was significant correlation between GATA3 expression with the tumor histological grade and degree muscle invasion. There was a weak or even negative expression in high‑grade, invasive than the low‑grade, non-invasive tumor [P= <0.001]. CK5/6 was positive and focally positive in 27.78% with a significant correlation of CK5/6 expression with tumor grade and muscularis propria invasion. On the other side, tumors with diffuse GATA3 expression had low CK5/6 expression. Conclusion: GATA3 and CK 5/6 should be used as sensitive and specific markers for UC. They can also be effectively used in the prediction of probable grade and tumor invasion in biopsied material with poor morphological characters; and thus, help in the future appropriate treatment.Marwa Abdel Moneim El KholyMohamed AliAhmed AboelsaadAhmed Abdel GawadAl-Azhar University, Faculty of Medicine (Damietta)articleurothelial carcinomatransurethral bladder tumor resectionmuscle-invasive bladder cancersgata‑3, ck 5/6immunohistochemicalMedicine (General)R5-920ENInternational Journal of Medical Arts, Vol 3, Iss 2, Pp 1306-1315 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	urothelial carcinoma transurethral bladder tumor resection muscle-invasive bladder cancers gata‑3, ck 5/6 immunohistochemical Medicine (General) R5-920
spellingShingle	urothelial carcinoma transurethral bladder tumor resection muscle-invasive bladder cancers gata‑3, ck 5/6 immunohistochemical Medicine (General) R5-920 Marwa Abdel Moneim El Kholy Mohamed Ali Ahmed Aboelsaad Ahmed Abdel Gawad GATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance
description	Background: Urothelial carcinomas [UCs] can be diagnostically challenging, particularly transurethral bladder tumors resection biopsies with limited material. Immunohistochemistry is a valuable instrument for diagnosis when morphology alone is inadequate. GATA-binding protein 3 [GATA3] and cytokeratin 5/6 [CK 5/6] contributes to the growth of UC. However, their correlative expression in UC and prognostic value has not been sufficiently investigated. The aim of the work: The current study aimed to evaluate the utility of GATA3 expression in UC and correlate that with CK 5/6 expression to verify different subtypes of UC and assess their prognostic significance. Methodology: Here, we immunohistochemically stained GATA3 and CK 5/6 in 90 UCs samples by transurethral bladder tumor resection in a retrospective study, between May 2018 and February 2020. All were histopathologically evaluated and immunohistochemically stained for GATA3 and CK 5/6, and then correlated them with the clinicopathological parameters to investigate their clinical significance. Results: GATA3 expression was seen in 76 patients [84.44 %]. There was significant correlation between GATA3 expression with the tumor histological grade and degree muscle invasion. There was a weak or even negative expression in high‑grade, invasive than the low‑grade, non-invasive tumor [P= <0.001]. CK5/6 was positive and focally positive in 27.78% with a significant correlation of CK5/6 expression with tumor grade and muscularis propria invasion. On the other side, tumors with diffuse GATA3 expression had low CK5/6 expression. Conclusion: GATA3 and CK 5/6 should be used as sensitive and specific markers for UC. They can also be effectively used in the prediction of probable grade and tumor invasion in biopsied material with poor morphological characters; and thus, help in the future appropriate treatment.
format	article
author	Marwa Abdel Moneim El Kholy Mohamed Ali Ahmed Aboelsaad Ahmed Abdel Gawad
author_facet	Marwa Abdel Moneim El Kholy Mohamed Ali Ahmed Aboelsaad Ahmed Abdel Gawad
author_sort	Marwa Abdel Moneim El Kholy
title	GATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance
title_short	GATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance
title_full	GATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance
title_fullStr	GATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance
title_full_unstemmed	GATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance
title_sort	gata3 and ck5/6 immunohistochemical expression in urothelial carcinoma: diagnostic, biological and prognostic significance
publisher	Al-Azhar University, Faculty of Medicine (Damietta)
publishDate	2021
url	https://doaj.org/article/9f4b954f71ba44779a551d8312ccf314
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_version_	1718385466427834368

GATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance

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