OVATE Family Protein PpOFP1 Physically Interacts With PpZFHD1 and Confers Salt Tolerance to Tomato and Yeast
The OVATE family protein (OFP) genes (OFPs) have been shown to respond to salt stress in plants. However, the regulatory mechanism for salt tolerance of the peach (Prunus persica) OFP gene PpOFP1 has not been elucidated. In this study, using yeast two-hybrid screening, we isolated a nucleus-localize...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:9f4bd092c8c7456c9b7442ce5bee23d72021-11-12T17:20:09ZOVATE Family Protein PpOFP1 Physically Interacts With PpZFHD1 and Confers Salt Tolerance to Tomato and Yeast1664-462X10.3389/fpls.2021.759955https://doaj.org/article/9f4bd092c8c7456c9b7442ce5bee23d72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.759955/fullhttps://doaj.org/toc/1664-462XThe OVATE family protein (OFP) genes (OFPs) have been shown to respond to salt stress in plants. However, the regulatory mechanism for salt tolerance of the peach (Prunus persica) OFP gene PpOFP1 has not been elucidated. In this study, using yeast two-hybrid screening, we isolated a nucleus-localized ZF-HD_dimer domain protein PpZFHD1, which interacts with the PpOFP1 protein in the peach cultivar “Zhongnongpan No.10”. A segmentation experiment further suggested that the interaction happens more specifically between the N-terminal, contains ZF-HD_dimer domain, of PpZFHD1 and the C-terminal, consists of OVATE domain, of PpOFP1. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) experiments indicate that transcription of these two genes are induced by 200 mmol/L (mM) NaCl treatment. Heterogeneous transformation experiments suggested that the growth status of transformed yeast strain over-expressing each of these two genes was more robust than that of control (CK). Furthermore, transgenic tomato plants over-expressing PpOFP1 were also more robust. They had a higher content of chlorophyll, soluble proteins, soluble sugars, and proline. Activities of the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in these plants were higher, and tissues from these plants exhibited a lower relative conductivity and malondialdehyde (MDA) content. These results suggest that PpOFP1 physically interacts with PpZFHD1 and confers salt tolerance to tomato and yeast, thus revealing a novel mechanism for regulating salt tolerance in peach and other perennial deciduous trees.Qiuping TanQiuping TanQiuping TanQiuping TanShan JiangNing WangNing WangNing WangXiao LiuXiao LiuXiao LiuXinhao ZhangXinhao ZhangXinhao ZhangBinbin WenBinbin WenBinbin WenYuhui FangYuhui FangYuhui FangHuajie HeHuajie HeHuajie HeXiude ChenXiude ChenXiude ChenXiling FuXiling FuXiling FuDongmei LiDongmei LiDongmei LiWei XiaoWei XiaoWei XiaoLing LiLing LiLing LiFrontiers Media S.A.articlePrunus persicasalt toleranceOVATE Family Protein PpOFP1ZF-HD_dimer domain protein PpZFHD1protein interactionPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Prunus persica salt tolerance OVATE Family Protein PpOFP1 ZF-HD_dimer domain protein PpZFHD1 protein interaction Plant culture SB1-1110 |
| spellingShingle |
Prunus persica salt tolerance OVATE Family Protein PpOFP1 ZF-HD_dimer domain protein PpZFHD1 protein interaction Plant culture SB1-1110 Qiuping Tan Qiuping Tan Qiuping Tan Qiuping Tan Shan Jiang Ning Wang Ning Wang Ning Wang Xiao Liu Xiao Liu Xiao Liu Xinhao Zhang Xinhao Zhang Xinhao Zhang Binbin Wen Binbin Wen Binbin Wen Yuhui Fang Yuhui Fang Yuhui Fang Huajie He Huajie He Huajie He Xiude Chen Xiude Chen Xiude Chen Xiling Fu Xiling Fu Xiling Fu Dongmei Li Dongmei Li Dongmei Li Wei Xiao Wei Xiao Wei Xiao Ling Li Ling Li Ling Li OVATE Family Protein PpOFP1 Physically Interacts With PpZFHD1 and Confers Salt Tolerance to Tomato and Yeast |
| description |
The OVATE family protein (OFP) genes (OFPs) have been shown to respond to salt stress in plants. However, the regulatory mechanism for salt tolerance of the peach (Prunus persica) OFP gene PpOFP1 has not been elucidated. In this study, using yeast two-hybrid screening, we isolated a nucleus-localized ZF-HD_dimer domain protein PpZFHD1, which interacts with the PpOFP1 protein in the peach cultivar “Zhongnongpan No.10”. A segmentation experiment further suggested that the interaction happens more specifically between the N-terminal, contains ZF-HD_dimer domain, of PpZFHD1 and the C-terminal, consists of OVATE domain, of PpOFP1. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) experiments indicate that transcription of these two genes are induced by 200 mmol/L (mM) NaCl treatment. Heterogeneous transformation experiments suggested that the growth status of transformed yeast strain over-expressing each of these two genes was more robust than that of control (CK). Furthermore, transgenic tomato plants over-expressing PpOFP1 were also more robust. They had a higher content of chlorophyll, soluble proteins, soluble sugars, and proline. Activities of the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in these plants were higher, and tissues from these plants exhibited a lower relative conductivity and malondialdehyde (MDA) content. These results suggest that PpOFP1 physically interacts with PpZFHD1 and confers salt tolerance to tomato and yeast, thus revealing a novel mechanism for regulating salt tolerance in peach and other perennial deciduous trees. |
| format |
article |
| author |
Qiuping Tan Qiuping Tan Qiuping Tan Qiuping Tan Shan Jiang Ning Wang Ning Wang Ning Wang Xiao Liu Xiao Liu Xiao Liu Xinhao Zhang Xinhao Zhang Xinhao Zhang Binbin Wen Binbin Wen Binbin Wen Yuhui Fang Yuhui Fang Yuhui Fang Huajie He Huajie He Huajie He Xiude Chen Xiude Chen Xiude Chen Xiling Fu Xiling Fu Xiling Fu Dongmei Li Dongmei Li Dongmei Li Wei Xiao Wei Xiao Wei Xiao Ling Li Ling Li Ling Li |
| author_facet |
Qiuping Tan Qiuping Tan Qiuping Tan Qiuping Tan Shan Jiang Ning Wang Ning Wang Ning Wang Xiao Liu Xiao Liu Xiao Liu Xinhao Zhang Xinhao Zhang Xinhao Zhang Binbin Wen Binbin Wen Binbin Wen Yuhui Fang Yuhui Fang Yuhui Fang Huajie He Huajie He Huajie He Xiude Chen Xiude Chen Xiude Chen Xiling Fu Xiling Fu Xiling Fu Dongmei Li Dongmei Li Dongmei Li Wei Xiao Wei Xiao Wei Xiao Ling Li Ling Li Ling Li |
| author_sort |
Qiuping Tan |
| title |
OVATE Family Protein PpOFP1 Physically Interacts With PpZFHD1 and Confers Salt Tolerance to Tomato and Yeast |
| title_short |
OVATE Family Protein PpOFP1 Physically Interacts With PpZFHD1 and Confers Salt Tolerance to Tomato and Yeast |
| title_full |
OVATE Family Protein PpOFP1 Physically Interacts With PpZFHD1 and Confers Salt Tolerance to Tomato and Yeast |
| title_fullStr |
OVATE Family Protein PpOFP1 Physically Interacts With PpZFHD1 and Confers Salt Tolerance to Tomato and Yeast |
| title_full_unstemmed |
OVATE Family Protein PpOFP1 Physically Interacts With PpZFHD1 and Confers Salt Tolerance to Tomato and Yeast |
| title_sort |
ovate family protein ppofp1 physically interacts with ppzfhd1 and confers salt tolerance to tomato and yeast |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/9f4bd092c8c7456c9b7442ce5bee23d7 |
| work_keys_str_mv |
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