Immune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism.

Formosan subterranean termites, Coptotermes formosanus Shiraki, live socially in microbial-rich habitats. To understand the molecular mechanism by which termites combat pathogenic microbes, a full-length normalized cDNA library and four Suppression Subtractive Hybridization (SSH) libraries were cons...

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Autores principales:	Abid Hussain, Yi-Feng Li, Yu Cheng, Yang Liu, Chuan-Cheng Chen, Shuo-Yang Wen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f4c970ee44545cdbd3ccb43f885818c
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spelling	oai:doaj.org-article:9f4c970ee44545cdbd3ccb43f885818c2021-11-18T07:37:28ZImmune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism.1932-620310.1371/journal.pone.0069543https://doaj.org/article/9f4c970ee44545cdbd3ccb43f885818c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874972/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Formosan subterranean termites, Coptotermes formosanus Shiraki, live socially in microbial-rich habitats. To understand the molecular mechanism by which termites combat pathogenic microbes, a full-length normalized cDNA library and four Suppression Subtractive Hybridization (SSH) libraries were constructed from termite workers infected with entomopathogenic fungi (Metarhizium anisopliae and Beauveria bassiana), Gram-positive Bacillus thuringiensis and Gram-negative Escherichia coli, and the libraries were analyzed. From the high quality normalized cDNA library, 439 immune-related sequences were identified. These sequences were categorized as pattern recognition receptors (47 sequences), signal modulators (52 sequences), signal transducers (137 sequences), effectors (39 sequences) and others (164 sequences). From the SSH libraries, 27, 17, 22 and 15 immune-related genes were identified from each SSH library treated with M. anisopliae, B. bassiana, B. thuringiensis and E. coli, respectively. When the normalized cDNA library was compared with the SSH libraries, 37 immune-related clusters were found in common; 56 clusters were identified in the SSH libraries, and 259 were identified in the normalized cDNA library. The immune-related gene expression pattern was further investigated using quantitative real time PCR (qPCR). Important immune-related genes were characterized, and their potential functions were discussed based on the integrated analysis of the results. We suggest that normalized cDNA and SSH libraries enable us to discover functional genes transcriptome. The results remarkably expand our knowledge about immune-inducible genes in C. formosanus Shiraki and enable the future development of novel control strategies for the management of Formosan subterranean termites.Abid HussainYi-Feng LiYu ChengYang LiuChuan-Cheng ChenShuo-Yang WenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69543 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abid Hussain Yi-Feng Li Yu Cheng Yang Liu Chuan-Cheng Chen Shuo-Yang Wen Immune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism.
description	Formosan subterranean termites, Coptotermes formosanus Shiraki, live socially in microbial-rich habitats. To understand the molecular mechanism by which termites combat pathogenic microbes, a full-length normalized cDNA library and four Suppression Subtractive Hybridization (SSH) libraries were constructed from termite workers infected with entomopathogenic fungi (Metarhizium anisopliae and Beauveria bassiana), Gram-positive Bacillus thuringiensis and Gram-negative Escherichia coli, and the libraries were analyzed. From the high quality normalized cDNA library, 439 immune-related sequences were identified. These sequences were categorized as pattern recognition receptors (47 sequences), signal modulators (52 sequences), signal transducers (137 sequences), effectors (39 sequences) and others (164 sequences). From the SSH libraries, 27, 17, 22 and 15 immune-related genes were identified from each SSH library treated with M. anisopliae, B. bassiana, B. thuringiensis and E. coli, respectively. When the normalized cDNA library was compared with the SSH libraries, 37 immune-related clusters were found in common; 56 clusters were identified in the SSH libraries, and 259 were identified in the normalized cDNA library. The immune-related gene expression pattern was further investigated using quantitative real time PCR (qPCR). Important immune-related genes were characterized, and their potential functions were discussed based on the integrated analysis of the results. We suggest that normalized cDNA and SSH libraries enable us to discover functional genes transcriptome. The results remarkably expand our knowledge about immune-inducible genes in C. formosanus Shiraki and enable the future development of novel control strategies for the management of Formosan subterranean termites.
format	article
author	Abid Hussain Yi-Feng Li Yu Cheng Yang Liu Chuan-Cheng Chen Shuo-Yang Wen
author_facet	Abid Hussain Yi-Feng Li Yu Cheng Yang Liu Chuan-Cheng Chen Shuo-Yang Wen
author_sort	Abid Hussain
title	Immune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism.
title_short	Immune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism.
title_full	Immune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism.
title_fullStr	Immune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism.
title_full_unstemmed	Immune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism.
title_sort	immune-related transcriptome of coptotermes formosanus shiraki workers: the defense mechanism.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9f4c970ee44545cdbd3ccb43f885818c
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_version_	1718423179812143104

Immune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism.

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