Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

Optimizing an enzyme usually requires testing thousands of variants, thus consuming large amounts of material and time. Here, the authors present a method that allows for measuring two different activities of the same enzyme simultaneously instead of doing two consecutive rounds of screening.

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Autores principales:	Fuqiang Ma, Meng Ting Chung, Yuan Yao, Robert Nidetz, Lap Man Lee, Allen P. Liu, Yan Feng, Katsuo Kurabayashi, Guang-Yu Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9f4f01f54d654f369fd23c54de3aefef
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spelling	oai:doaj.org-article:9f4f01f54d654f369fd23c54de3aefef2021-12-02T17:33:17ZEfficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform10.1038/s41467-018-03492-62041-1723https://doaj.org/article/9f4f01f54d654f369fd23c54de3aefef2018-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03492-6https://doaj.org/toc/2041-1723Optimizing an enzyme usually requires testing thousands of variants, thus consuming large amounts of material and time. Here, the authors present a method that allows for measuring two different activities of the same enzyme simultaneously instead of doing two consecutive rounds of screening.Fuqiang MaMeng Ting ChungYuan YaoRobert NidetzLap Man LeeAllen P. LiuYan FengKatsuo KurabayashiGuang-Yu YangNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Fuqiang Ma Meng Ting Chung Yuan Yao Robert Nidetz Lap Man Lee Allen P. Liu Yan Feng Katsuo Kurabayashi Guang-Yu Yang Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
description	Optimizing an enzyme usually requires testing thousands of variants, thus consuming large amounts of material and time. Here, the authors present a method that allows for measuring two different activities of the same enzyme simultaneously instead of doing two consecutive rounds of screening.
format	article
author	Fuqiang Ma Meng Ting Chung Yuan Yao Robert Nidetz Lap Man Lee Allen P. Liu Yan Feng Katsuo Kurabayashi Guang-Yu Yang
author_facet	Fuqiang Ma Meng Ting Chung Yuan Yao Robert Nidetz Lap Man Lee Allen P. Liu Yan Feng Katsuo Kurabayashi Guang-Yu Yang
author_sort	Fuqiang Ma
title	Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
title_short	Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
title_full	Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
title_fullStr	Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
title_full_unstemmed	Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
title_sort	efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9f4f01f54d654f369fd23c54de3aefef
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Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

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