Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

Optimizing an enzyme usually requires testing thousands of variants, thus consuming large amounts of material and time. Here, the authors present a method that allows for measuring two different activities of the same enzyme simultaneously instead of doing two consecutive rounds of screening.

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Bibliographic Details
Main Authors: Fuqiang Ma, Meng Ting Chung, Yuan Yao, Robert Nidetz, Lap Man Lee, Allen P. Liu, Yan Feng, Katsuo Kurabayashi, Guang-Yu Yang
Format: article
Language:EN
Published: Nature Portfolio 2018
Subjects:
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Online Access:https://doaj.org/article/9f4f01f54d654f369fd23c54de3aefef
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