FTIR Spectroscopy as a Tool to Study Age-Related Changes in Cardiac and Skeletal Muscle of Female C57BL/6J Mice

Studying aging is important to further understand the molecular mechanisms underlying this physiological process and, ideally, to identify a panel of aging biomarkers. Animals, in particular mice, are often used in aging studies, since they mimic important features of human aging, age quickly, and a...

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Autores principales:	Sandra Magalhães, Idália Almeida, Filipa Martins, Fátima Camões, Ana R. Soares, Brian J. Goodfellow, Sandra Rebelo, Alexandra Nunes
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	aging fingerprint aging muscle spectroscopic profile Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/9f5323747db449659fa173227da3604d
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spelling	oai:doaj.org-article:9f5323747db449659fa173227da3604d2021-11-11T18:25:44ZFTIR Spectroscopy as a Tool to Study Age-Related Changes in Cardiac and Skeletal Muscle of Female C57BL/6J Mice10.3390/molecules262164101420-3049https://doaj.org/article/9f5323747db449659fa173227da3604d2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6410https://doaj.org/toc/1420-3049Studying aging is important to further understand the molecular mechanisms underlying this physiological process and, ideally, to identify a panel of aging biomarkers. Animals, in particular mice, are often used in aging studies, since they mimic important features of human aging, age quickly, and are easy to manipulate. The present work describes the use of Fourier Transform Infrared (FTIR) spectroscopy to identify an age-related spectroscopic profile of the cardiac and skeletal muscle tissues of C57BL/6J female mice. We acquired ATR-FTIR spectra of cardiac and skeletal muscle at four different ages: 6; 12; 17 and 24 months (10 samples at each age) and analyzed the data using multivariate statistical tools (PCA and PLS) and peak intensity analyses. The results suggest deep changes in protein secondary structure in 24-month-old mice compared to both tissues in 6-month-old mice. Oligomeric structures decreased with age in both tissues, while intermolecular β-sheet structures increased with aging in cardiac muscle but not in skeletal muscle. Despite FTIR spectroscopy being unable to identify the proteins responsible for these conformational changes, this study gives insights into the potential of FTIR to monitor the aging process and identify an age-specific spectroscopic signature.Sandra MagalhãesIdália AlmeidaFilipa MartinsFátima CamõesAna R. SoaresBrian J. GoodfellowSandra RebeloAlexandra NunesMDPI AGarticleaging fingerprintaging musclespectroscopic profileOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6410, p 6410 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aging fingerprint aging muscle spectroscopic profile Organic chemistry QD241-441
spellingShingle	aging fingerprint aging muscle spectroscopic profile Organic chemistry QD241-441 Sandra Magalhães Idália Almeida Filipa Martins Fátima Camões Ana R. Soares Brian J. Goodfellow Sandra Rebelo Alexandra Nunes FTIR Spectroscopy as a Tool to Study Age-Related Changes in Cardiac and Skeletal Muscle of Female C57BL/6J Mice
description	Studying aging is important to further understand the molecular mechanisms underlying this physiological process and, ideally, to identify a panel of aging biomarkers. Animals, in particular mice, are often used in aging studies, since they mimic important features of human aging, age quickly, and are easy to manipulate. The present work describes the use of Fourier Transform Infrared (FTIR) spectroscopy to identify an age-related spectroscopic profile of the cardiac and skeletal muscle tissues of C57BL/6J female mice. We acquired ATR-FTIR spectra of cardiac and skeletal muscle at four different ages: 6; 12; 17 and 24 months (10 samples at each age) and analyzed the data using multivariate statistical tools (PCA and PLS) and peak intensity analyses. The results suggest deep changes in protein secondary structure in 24-month-old mice compared to both tissues in 6-month-old mice. Oligomeric structures decreased with age in both tissues, while intermolecular β-sheet structures increased with aging in cardiac muscle but not in skeletal muscle. Despite FTIR spectroscopy being unable to identify the proteins responsible for these conformational changes, this study gives insights into the potential of FTIR to monitor the aging process and identify an age-specific spectroscopic signature.
format	article
author	Sandra Magalhães Idália Almeida Filipa Martins Fátima Camões Ana R. Soares Brian J. Goodfellow Sandra Rebelo Alexandra Nunes
author_facet	Sandra Magalhães Idália Almeida Filipa Martins Fátima Camões Ana R. Soares Brian J. Goodfellow Sandra Rebelo Alexandra Nunes
author_sort	Sandra Magalhães
title	FTIR Spectroscopy as a Tool to Study Age-Related Changes in Cardiac and Skeletal Muscle of Female C57BL/6J Mice
title_short	FTIR Spectroscopy as a Tool to Study Age-Related Changes in Cardiac and Skeletal Muscle of Female C57BL/6J Mice
title_full	FTIR Spectroscopy as a Tool to Study Age-Related Changes in Cardiac and Skeletal Muscle of Female C57BL/6J Mice
title_fullStr	FTIR Spectroscopy as a Tool to Study Age-Related Changes in Cardiac and Skeletal Muscle of Female C57BL/6J Mice
title_full_unstemmed	FTIR Spectroscopy as a Tool to Study Age-Related Changes in Cardiac and Skeletal Muscle of Female C57BL/6J Mice
title_sort	ftir spectroscopy as a tool to study age-related changes in cardiac and skeletal muscle of female c57bl/6j mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9f5323747db449659fa173227da3604d
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FTIR Spectroscopy as a Tool to Study Age-Related Changes in Cardiac and Skeletal Muscle of Female C57BL/6J Mice

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