New insights into the proteins interacting with the promoters of silkworm fibroin genes

Abstract The silkworm, Bombyx mori, is a silk-producing insect that has contributed greatly to human society. The silk gland of B. mori is a specialized organ responsible for synthesizing silk fibroin and sericin proteins under control of numerous factors. However, which factors are involved in dire...

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Autores principales:	Yan Ma, Qin Luo, Yao Ou, Yiyun Tang, Wenhui Zeng, Haomiao Wang, Jie Hu, Hanfu Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f5459cb35874e6e81e18c7c08b4ee29
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spelling	oai:doaj.org-article:9f5459cb35874e6e81e18c7c08b4ee292021-12-02T14:53:34ZNew insights into the proteins interacting with the promoters of silkworm fibroin genes10.1038/s41598-021-95400-02045-2322https://doaj.org/article/9f5459cb35874e6e81e18c7c08b4ee292021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95400-0https://doaj.org/toc/2045-2322Abstract The silkworm, Bombyx mori, is a silk-producing insect that has contributed greatly to human society. The silk gland of B. mori is a specialized organ responsible for synthesizing silk fibroin and sericin proteins under control of numerous factors. However, which factors are involved in direct silk protein synthesis regulation remains largely unknown. We report the identification of promoter-interacting proteins (PIPs) necessary for the regulation of genes encoding fibroin proteins, including the fibroin heavy chain (fibH), fibroin light chain (fibL), and a 25-kD polypeptide protein (P25). In the fourth larval molting stage (M4) or day 5 fifth-instar larvae (L5D5), a total of 198, 292, and 247 or 330, 305, and 460 proteins interacting with the promoter region of fibH, fibL and P25, respectively, were identified from the posterior silk gland by DNA pull-down combined with mass spectrometry. Many PIPs were particularly involved in ribosome- and metabolism-related pathways. Additionally, 135 and 212 proteins were identified as common PIPs of fibH, fibL and P25 in M4 and L5D5, respectively. Among all PIPs, we identified 31 potential transcription factors, such as Y-box and poly A-binding proteins, which play roles in nucleotide binding, ATP binding, or protein folding. This study provides the first in-depth profile of proteins interacting with fibroin gene promoters and contributes to a better understanding of silk protein synthesis regulation.Yan MaQin LuoYao OuYiyun TangWenhui ZengHaomiao WangJie HuHanfu XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Ma Qin Luo Yao Ou Yiyun Tang Wenhui Zeng Haomiao Wang Jie Hu Hanfu Xu New insights into the proteins interacting with the promoters of silkworm fibroin genes
description	Abstract The silkworm, Bombyx mori, is a silk-producing insect that has contributed greatly to human society. The silk gland of B. mori is a specialized organ responsible for synthesizing silk fibroin and sericin proteins under control of numerous factors. However, which factors are involved in direct silk protein synthesis regulation remains largely unknown. We report the identification of promoter-interacting proteins (PIPs) necessary for the regulation of genes encoding fibroin proteins, including the fibroin heavy chain (fibH), fibroin light chain (fibL), and a 25-kD polypeptide protein (P25). In the fourth larval molting stage (M4) or day 5 fifth-instar larvae (L5D5), a total of 198, 292, and 247 or 330, 305, and 460 proteins interacting with the promoter region of fibH, fibL and P25, respectively, were identified from the posterior silk gland by DNA pull-down combined with mass spectrometry. Many PIPs were particularly involved in ribosome- and metabolism-related pathways. Additionally, 135 and 212 proteins were identified as common PIPs of fibH, fibL and P25 in M4 and L5D5, respectively. Among all PIPs, we identified 31 potential transcription factors, such as Y-box and poly A-binding proteins, which play roles in nucleotide binding, ATP binding, or protein folding. This study provides the first in-depth profile of proteins interacting with fibroin gene promoters and contributes to a better understanding of silk protein synthesis regulation.
format	article
author	Yan Ma Qin Luo Yao Ou Yiyun Tang Wenhui Zeng Haomiao Wang Jie Hu Hanfu Xu
author_facet	Yan Ma Qin Luo Yao Ou Yiyun Tang Wenhui Zeng Haomiao Wang Jie Hu Hanfu Xu
author_sort	Yan Ma
title	New insights into the proteins interacting with the promoters of silkworm fibroin genes
title_short	New insights into the proteins interacting with the promoters of silkworm fibroin genes
title_full	New insights into the proteins interacting with the promoters of silkworm fibroin genes
title_fullStr	New insights into the proteins interacting with the promoters of silkworm fibroin genes
title_full_unstemmed	New insights into the proteins interacting with the promoters of silkworm fibroin genes
title_sort	new insights into the proteins interacting with the promoters of silkworm fibroin genes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9f5459cb35874e6e81e18c7c08b4ee29
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New insights into the proteins interacting with the promoters of silkworm fibroin genes

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