FFPE samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas.

During surgical procedures for gliomas, tissue material obtained from cavitational ultrasonic surgical aspirators (CUSAs) is generally discarded but can actually exceed the amount and quality of certain tumour core resections (TCRs). Despite reports indicating the suitability of CUSA-derived materia...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cristina Alenda, Estefanía Rojas, Luis M Valor
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f561cba2e9f428bbda85a8e9027ed6a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9f561cba2e9f428bbda85a8e9027ed6a
record_format	dspace
spelling	oai:doaj.org-article:9f561cba2e9f428bbda85a8e9027ed6a2021-12-02T20:04:57ZFFPE samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas.1932-620310.1371/journal.pone.0255168https://doaj.org/article/9f561cba2e9f428bbda85a8e9027ed6a2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255168https://doaj.org/toc/1932-6203During surgical procedures for gliomas, tissue material obtained from cavitational ultrasonic surgical aspirators (CUSAs) is generally discarded but can actually exceed the amount and quality of certain tumour core resections (TCRs). Despite reports indicating the suitability of CUSA-derived material for diagnosis and research, its use is still marginal. We extended these conclusions to formalin-fixed, paraffin-embedded (FFPE) samples, the most common format for archival tumour tissue in anatomical pathology departments, by conducting for the first time RNA-seq analysis in CUSA aspirates. We compared the molecular diagnosis of somatic mutations used in the clinical routine and the gene expression profiles of fixed solid material from CUSA aspirates and TCRs from the same patients in selected gliomas encompassing grades II to IV. Despite the characteristic heterogeneity of gliomas, we found substantial similarities between the corresponding aspirates and TCRs that included transcriptional signatures associated with glioma subtypes. Based on these results, we confirmed that CUSA-fixed biomaterials from glioma surgeries are appropriate for downstream applications and biomarkers screening.Cristina AlendaEstefanía RojasLuis M ValorPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255168 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristina Alenda Estefanía Rojas Luis M Valor FFPE samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas.
description	During surgical procedures for gliomas, tissue material obtained from cavitational ultrasonic surgical aspirators (CUSAs) is generally discarded but can actually exceed the amount and quality of certain tumour core resections (TCRs). Despite reports indicating the suitability of CUSA-derived material for diagnosis and research, its use is still marginal. We extended these conclusions to formalin-fixed, paraffin-embedded (FFPE) samples, the most common format for archival tumour tissue in anatomical pathology departments, by conducting for the first time RNA-seq analysis in CUSA aspirates. We compared the molecular diagnosis of somatic mutations used in the clinical routine and the gene expression profiles of fixed solid material from CUSA aspirates and TCRs from the same patients in selected gliomas encompassing grades II to IV. Despite the characteristic heterogeneity of gliomas, we found substantial similarities between the corresponding aspirates and TCRs that included transcriptional signatures associated with glioma subtypes. Based on these results, we confirmed that CUSA-fixed biomaterials from glioma surgeries are appropriate for downstream applications and biomarkers screening.
format	article
author	Cristina Alenda Estefanía Rojas Luis M Valor
author_facet	Cristina Alenda Estefanía Rojas Luis M Valor
author_sort	Cristina Alenda
title	FFPE samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas.
title_short	FFPE samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas.
title_full	FFPE samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas.
title_fullStr	FFPE samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas.
title_full_unstemmed	FFPE samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas.
title_sort	ffpe samples from cavitational ultrasonic surgical aspirates are suitable for rna profiling of gliomas.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9f561cba2e9f428bbda85a8e9027ed6a
work_keys_str_mv	AT cristinaalenda ffpesamplesfromcavitationalultrasonicsurgicalaspiratesaresuitableforrnaprofilingofgliomas AT estefaniarojas ffpesamplesfromcavitationalultrasonicsurgicalaspiratesaresuitableforrnaprofilingofgliomas AT luismvalor ffpesamplesfromcavitationalultrasonicsurgicalaspiratesaresuitableforrnaprofilingofgliomas
_version_	1718375559104299008

FFPE samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas.

Ejemplares similares