An alternative approach to produce versatile retinal organoids with accelerated ganglion cell development

Abstract Genetically complex ocular neuropathies, such as glaucoma, are a major cause of visual impairment worldwide. There is a growing need to generate suitable human representative in vitro and in vivo models, as there is no effective treatment available once damage has occured. Retinal organoids...

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Autores principales:	Philip E. Wagstaff, Anneloor L. M. A. ten Asbroek, Jacoline B. ten Brink, Nomdo M. Jansonius, Arthur A. B. Bergen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f613427ee484beea800df072f76ad62
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spelling	oai:doaj.org-article:9f613427ee484beea800df072f76ad622021-12-02T14:12:42ZAn alternative approach to produce versatile retinal organoids with accelerated ganglion cell development10.1038/s41598-020-79651-x2045-2322https://doaj.org/article/9f613427ee484beea800df072f76ad622021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79651-xhttps://doaj.org/toc/2045-2322Abstract Genetically complex ocular neuropathies, such as glaucoma, are a major cause of visual impairment worldwide. There is a growing need to generate suitable human representative in vitro and in vivo models, as there is no effective treatment available once damage has occured. Retinal organoids are increasingly being used for experimental gene therapy, stem cell replacement therapy and small molecule therapy. There are multiple protocols for the development of retinal organoids available, however, one potential drawback of the current methods is that the organoids can take between 6 weeks and 12 months on average to develop and mature, depending on the specific cell type wanted. Here, we describe and characterise a protocol focused on the generation of retinal ganglion cells within an accelerated four week timeframe without any external small molecules or growth factors. Subsequent long term cultures yield fully differentiated organoids displaying all major retinal cell types. RPE, Horizontal, Amacrine and Photoreceptors cells were generated using external factors to maintain lamination.Philip E. WagstaffAnneloor L. M. A. ten AsbroekJacoline B. ten BrinkNomdo M. JansoniusArthur A. B. BergenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Philip E. Wagstaff Anneloor L. M. A. ten Asbroek Jacoline B. ten Brink Nomdo M. Jansonius Arthur A. B. Bergen An alternative approach to produce versatile retinal organoids with accelerated ganglion cell development
description	Abstract Genetically complex ocular neuropathies, such as glaucoma, are a major cause of visual impairment worldwide. There is a growing need to generate suitable human representative in vitro and in vivo models, as there is no effective treatment available once damage has occured. Retinal organoids are increasingly being used for experimental gene therapy, stem cell replacement therapy and small molecule therapy. There are multiple protocols for the development of retinal organoids available, however, one potential drawback of the current methods is that the organoids can take between 6 weeks and 12 months on average to develop and mature, depending on the specific cell type wanted. Here, we describe and characterise a protocol focused on the generation of retinal ganglion cells within an accelerated four week timeframe without any external small molecules or growth factors. Subsequent long term cultures yield fully differentiated organoids displaying all major retinal cell types. RPE, Horizontal, Amacrine and Photoreceptors cells were generated using external factors to maintain lamination.
format	article
author	Philip E. Wagstaff Anneloor L. M. A. ten Asbroek Jacoline B. ten Brink Nomdo M. Jansonius Arthur A. B. Bergen
author_facet	Philip E. Wagstaff Anneloor L. M. A. ten Asbroek Jacoline B. ten Brink Nomdo M. Jansonius Arthur A. B. Bergen
author_sort	Philip E. Wagstaff
title	An alternative approach to produce versatile retinal organoids with accelerated ganglion cell development
title_short	An alternative approach to produce versatile retinal organoids with accelerated ganglion cell development
title_full	An alternative approach to produce versatile retinal organoids with accelerated ganglion cell development
title_fullStr	An alternative approach to produce versatile retinal organoids with accelerated ganglion cell development
title_full_unstemmed	An alternative approach to produce versatile retinal organoids with accelerated ganglion cell development
title_sort	alternative approach to produce versatile retinal organoids with accelerated ganglion cell development
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9f613427ee484beea800df072f76ad62
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An alternative approach to produce versatile retinal organoids with accelerated ganglion cell development

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