Continual conscious bioluminescent imaging in freely moving somatotransgenic mice

Abstract Luciferase bioimaging in living animals is increasingly being applied in many fields of biomedical research. Rodent imaging usually involves anaesthetising the animal during data capture, however, the biological consequences of anaesthesia have been largely overlooked. We have evaluated luc...

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Autores principales:	Rajvinder Karda, Dany P. Perocheau, Natalie Suff, Joanne Ng, Juliette M. K. M. Delhove, Suzanne M. K. Buckley, Samantha Richards, John R. Counsell, Henrik Hagberg, Mark R. Johnson, Tristan R. McKay, Simon N. Waddington
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f6f64e65ad641139897de92958624f7
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spelling	oai:doaj.org-article:9f6f64e65ad641139897de92958624f72021-12-02T11:52:36ZContinual conscious bioluminescent imaging in freely moving somatotransgenic mice10.1038/s41598-017-06696-w2045-2322https://doaj.org/article/9f6f64e65ad641139897de92958624f72017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06696-whttps://doaj.org/toc/2045-2322Abstract Luciferase bioimaging in living animals is increasingly being applied in many fields of biomedical research. Rodent imaging usually involves anaesthetising the animal during data capture, however, the biological consequences of anaesthesia have been largely overlooked. We have evaluated luciferase bioimaging in conscious, unrestrained mice after neonatal intracranial or intravascular administration of lentiviral, luciferase reporter cassettes (biosensors); we present real-time analyses from the first day of life to adulthood. Anaesthetics have been shown to exert both neurotoxic and neuroprotective effects during development and in models of brain injury. Mice subjected to bioimaging after neonatal intracranial or intravascular administration of biosensors, targeting the brain and liver retrospectively showed no significant difference in luciferase expression when conscious or unconscious throughout development. We applied conscious bioimaging to the assessment of NFκB and STAT3 transcription factor activated reporters during the earliest stages of development in living, unrestrained pups. Our data showed unique longitudinal activities for NFκB and STAT3 in the brain of conscious mice. Conscious bioimaging was applied to a neonatal mouse model of cerebral palsy (Hypoxic-Ischaemic Encephalopathy). Imaging of NFκB reporter before and after surgery showed a significant increase in luciferase expression, coinciding with secondary energy failure, in lesioned mice compared to controls.Rajvinder KardaDany P. PerocheauNatalie SuffJoanne NgJuliette M. K. M. DelhoveSuzanne M. K. BuckleySamantha RichardsJohn R. CounsellHenrik HagbergMark R. JohnsonTristan R. McKaySimon N. WaddingtonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rajvinder Karda Dany P. Perocheau Natalie Suff Joanne Ng Juliette M. K. M. Delhove Suzanne M. K. Buckley Samantha Richards John R. Counsell Henrik Hagberg Mark R. Johnson Tristan R. McKay Simon N. Waddington Continual conscious bioluminescent imaging in freely moving somatotransgenic mice
description	Abstract Luciferase bioimaging in living animals is increasingly being applied in many fields of biomedical research. Rodent imaging usually involves anaesthetising the animal during data capture, however, the biological consequences of anaesthesia have been largely overlooked. We have evaluated luciferase bioimaging in conscious, unrestrained mice after neonatal intracranial or intravascular administration of lentiviral, luciferase reporter cassettes (biosensors); we present real-time analyses from the first day of life to adulthood. Anaesthetics have been shown to exert both neurotoxic and neuroprotective effects during development and in models of brain injury. Mice subjected to bioimaging after neonatal intracranial or intravascular administration of biosensors, targeting the brain and liver retrospectively showed no significant difference in luciferase expression when conscious or unconscious throughout development. We applied conscious bioimaging to the assessment of NFκB and STAT3 transcription factor activated reporters during the earliest stages of development in living, unrestrained pups. Our data showed unique longitudinal activities for NFκB and STAT3 in the brain of conscious mice. Conscious bioimaging was applied to a neonatal mouse model of cerebral palsy (Hypoxic-Ischaemic Encephalopathy). Imaging of NFκB reporter before and after surgery showed a significant increase in luciferase expression, coinciding with secondary energy failure, in lesioned mice compared to controls.
format	article
author	Rajvinder Karda Dany P. Perocheau Natalie Suff Joanne Ng Juliette M. K. M. Delhove Suzanne M. K. Buckley Samantha Richards John R. Counsell Henrik Hagberg Mark R. Johnson Tristan R. McKay Simon N. Waddington
author_facet	Rajvinder Karda Dany P. Perocheau Natalie Suff Joanne Ng Juliette M. K. M. Delhove Suzanne M. K. Buckley Samantha Richards John R. Counsell Henrik Hagberg Mark R. Johnson Tristan R. McKay Simon N. Waddington
author_sort	Rajvinder Karda
title	Continual conscious bioluminescent imaging in freely moving somatotransgenic mice
title_short	Continual conscious bioluminescent imaging in freely moving somatotransgenic mice
title_full	Continual conscious bioluminescent imaging in freely moving somatotransgenic mice
title_fullStr	Continual conscious bioluminescent imaging in freely moving somatotransgenic mice
title_full_unstemmed	Continual conscious bioluminescent imaging in freely moving somatotransgenic mice
title_sort	continual conscious bioluminescent imaging in freely moving somatotransgenic mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9f6f64e65ad641139897de92958624f7
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Continual conscious bioluminescent imaging in freely moving somatotransgenic mice

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