Patch clamp-assisted single neuron lipidomics

Abstract Our understanding of the physiological and pathological functions of brain lipids is limited by the inability to analyze these molecules at cellular resolution. Here, we present a method that enables the detection of lipids in identified single neurons from live mammalian brains. Neuronal c...

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Autores principales:	Collin B. Merrill, Abdul Basit, Andrea Armirotti, Yousheng Jia, Christine M. Gall, Gary Lynch, Daniele Piomelli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f790afc890d4acf944d1f4e0b9ee98f
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spelling	oai:doaj.org-article:9f790afc890d4acf944d1f4e0b9ee98f2021-12-02T11:40:52ZPatch clamp-assisted single neuron lipidomics10.1038/s41598-017-05607-32045-2322https://doaj.org/article/9f790afc890d4acf944d1f4e0b9ee98f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05607-3https://doaj.org/toc/2045-2322Abstract Our understanding of the physiological and pathological functions of brain lipids is limited by the inability to analyze these molecules at cellular resolution. Here, we present a method that enables the detection of lipids in identified single neurons from live mammalian brains. Neuronal cell bodies are captured from perfused mouse brain slices by patch clamping, and lipids are analyzed using an optimized nanoflow liquid chromatography/mass spectrometry protocol. In a first application of the method, we identified more than 40 lipid species from dentate gyrus granule cells and CA1 pyramidal neurons of the hippocampus. This survey revealed substantial lipid profile differences between neurons and whole brain tissue, as well as between resting and physiologically stimulated neurons. The results suggest that patch clamp-assisted single neuron lipidomics could be broadly applied to investigate neuronal lipid homeostasis in healthy and diseased brains.Collin B. MerrillAbdul BasitAndrea ArmirottiYousheng JiaChristine M. GallGary LynchDaniele PiomelliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Collin B. Merrill Abdul Basit Andrea Armirotti Yousheng Jia Christine M. Gall Gary Lynch Daniele Piomelli Patch clamp-assisted single neuron lipidomics
description	Abstract Our understanding of the physiological and pathological functions of brain lipids is limited by the inability to analyze these molecules at cellular resolution. Here, we present a method that enables the detection of lipids in identified single neurons from live mammalian brains. Neuronal cell bodies are captured from perfused mouse brain slices by patch clamping, and lipids are analyzed using an optimized nanoflow liquid chromatography/mass spectrometry protocol. In a first application of the method, we identified more than 40 lipid species from dentate gyrus granule cells and CA1 pyramidal neurons of the hippocampus. This survey revealed substantial lipid profile differences between neurons and whole brain tissue, as well as between resting and physiologically stimulated neurons. The results suggest that patch clamp-assisted single neuron lipidomics could be broadly applied to investigate neuronal lipid homeostasis in healthy and diseased brains.
format	article
author	Collin B. Merrill Abdul Basit Andrea Armirotti Yousheng Jia Christine M. Gall Gary Lynch Daniele Piomelli
author_facet	Collin B. Merrill Abdul Basit Andrea Armirotti Yousheng Jia Christine M. Gall Gary Lynch Daniele Piomelli
author_sort	Collin B. Merrill
title	Patch clamp-assisted single neuron lipidomics
title_short	Patch clamp-assisted single neuron lipidomics
title_full	Patch clamp-assisted single neuron lipidomics
title_fullStr	Patch clamp-assisted single neuron lipidomics
title_full_unstemmed	Patch clamp-assisted single neuron lipidomics
title_sort	patch clamp-assisted single neuron lipidomics
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9f790afc890d4acf944d1f4e0b9ee98f
work_keys_str_mv	AT collinbmerrill patchclampassistedsingleneuronlipidomics AT abdulbasit patchclampassistedsingleneuronlipidomics AT andreaarmirotti patchclampassistedsingleneuronlipidomics AT youshengjia patchclampassistedsingleneuronlipidomics AT christinemgall patchclampassistedsingleneuronlipidomics AT garylynch patchclampassistedsingleneuronlipidomics AT danielepiomelli patchclampassistedsingleneuronlipidomics
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Patch clamp-assisted single neuron lipidomics

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