A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes

Abstract Mononuclear phagocytes (MNPs) participate in inflammation and repair after kidney injury, reflecting their complex nature. Dissection into refined functional subunits has been challenging and would benefit understanding of renal pathologies. Flow cytometric approaches are limited to classif...

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Autores principales:	Johannes Nordlohne, Ilona Hulsmann, Svenja Schwafertz, Jasmin Zgrajek, Manuel Grundmann, Sibylle von Vietinghoff, Frank Eitner, Michael S. Becker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f7f1171fe0e426d9a83fd8ce3509300
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spelling	oai:doaj.org-article:9f7f1171fe0e426d9a83fd8ce35093002021-12-02T18:02:55ZA flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes10.1038/s41598-021-92784-x2045-2322https://doaj.org/article/9f7f1171fe0e426d9a83fd8ce35093002021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92784-xhttps://doaj.org/toc/2045-2322Abstract Mononuclear phagocytes (MNPs) participate in inflammation and repair after kidney injury, reflecting their complex nature. Dissection into refined functional subunits has been challenging and would benefit understanding of renal pathologies. Flow cytometric approaches are limited to classifications of either different MNP subsets or functional state. We sought to combine these two dimensions in one protocol that considers functional heterogeneity in each MNP subset. We identified five distinct renal MNP subsets based on a previously described strategy. In vitro polarization of bone marrow-derived macrophages (BMDM) into M1- and M2-like cells suggested functional distinction of CD86 + MHCII + CD206- and CD206 + cells. Combination of both distinction methods identified CD86 + MHCII + CD206- and CD206 + cells in all five MNP subsets, revealing their heterologous nature. Our approach revealed that MNP composition and their functional segmentation varied between different mouse models of kidney injury and, moreover, was dynamically regulated in a time-dependent manner. CD206 + cells from three analyzed MNP subsets had a higher ex vivo phagocytic capacity than CD86 + MHCII + CD206- counterparts, indicating functional uniqueness of each subset. In conclusion, our novel flow cytometric approach refines insights into renal MNP heterogeneity and therefore could benefit mechanistic understanding of renal pathology.Johannes NordlohneIlona HulsmannSvenja SchwafertzJasmin ZgrajekManuel GrundmannSibylle von VietinghoffFrank EitnerMichael S. BeckerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johannes Nordlohne Ilona Hulsmann Svenja Schwafertz Jasmin Zgrajek Manuel Grundmann Sibylle von Vietinghoff Frank Eitner Michael S. Becker A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes
description	Abstract Mononuclear phagocytes (MNPs) participate in inflammation and repair after kidney injury, reflecting their complex nature. Dissection into refined functional subunits has been challenging and would benefit understanding of renal pathologies. Flow cytometric approaches are limited to classifications of either different MNP subsets or functional state. We sought to combine these two dimensions in one protocol that considers functional heterogeneity in each MNP subset. We identified five distinct renal MNP subsets based on a previously described strategy. In vitro polarization of bone marrow-derived macrophages (BMDM) into M1- and M2-like cells suggested functional distinction of CD86 + MHCII + CD206- and CD206 + cells. Combination of both distinction methods identified CD86 + MHCII + CD206- and CD206 + cells in all five MNP subsets, revealing their heterologous nature. Our approach revealed that MNP composition and their functional segmentation varied between different mouse models of kidney injury and, moreover, was dynamically regulated in a time-dependent manner. CD206 + cells from three analyzed MNP subsets had a higher ex vivo phagocytic capacity than CD86 + MHCII + CD206- counterparts, indicating functional uniqueness of each subset. In conclusion, our novel flow cytometric approach refines insights into renal MNP heterogeneity and therefore could benefit mechanistic understanding of renal pathology.
format	article
author	Johannes Nordlohne Ilona Hulsmann Svenja Schwafertz Jasmin Zgrajek Manuel Grundmann Sibylle von Vietinghoff Frank Eitner Michael S. Becker
author_facet	Johannes Nordlohne Ilona Hulsmann Svenja Schwafertz Jasmin Zgrajek Manuel Grundmann Sibylle von Vietinghoff Frank Eitner Michael S. Becker
author_sort	Johannes Nordlohne
title	A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes
title_short	A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes
title_full	A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes
title_fullStr	A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes
title_full_unstemmed	A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes
title_sort	flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9f7f1171fe0e426d9a83fd8ce3509300
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A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes

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