Chemically imaging bacteria with super-resolution SERS on ultra-thin silver substrates

Abstract Plasmonic hotspots generate a blinking Surface Enhanced Raman Spectroscopy (SERS) effect that can be processed using Stochastic Optical Reconstruction Microscopy (STORM) algorithms for super-resolved imaging. Furthermore, by imaging through a diffraction grating, STORM algorithms can be mod...

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Autores principales:	Aeli P. Olson, Kelsey B. Spies, Anna C. Browning, Paula A. G. Soneral, Nathan C. Lindquist
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f820792733b4536b5fe44b5456ff652
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spelling	oai:doaj.org-article:9f820792733b4536b5fe44b5456ff6522021-12-02T11:40:45ZChemically imaging bacteria with super-resolution SERS on ultra-thin silver substrates10.1038/s41598-017-08915-w2045-2322https://doaj.org/article/9f820792733b4536b5fe44b5456ff6522017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08915-whttps://doaj.org/toc/2045-2322Abstract Plasmonic hotspots generate a blinking Surface Enhanced Raman Spectroscopy (SERS) effect that can be processed using Stochastic Optical Reconstruction Microscopy (STORM) algorithms for super-resolved imaging. Furthermore, by imaging through a diffraction grating, STORM algorithms can be modified to extract a full SERS spectrum, thereby capturing spectral as well as spatial content simultaneously. Here we demonstrate SERS and STORM combined in this way for super-resolved chemical imaging using an ultra-thin silver substrate. Images of gram-positive and gram-negative bacteria taken with this technique show excellent agreement with scanning electron microscope images, high spatial resolution at <50 nm, and spectral SERS content that can be correlated to different regions. This may be used to identify unique chemical signatures of various cells. Finally, because we image through as-deposited, ultra-thin silver films, this technique requires no nanofabrication beyond a single deposition and looks at the cell samples from below. This allows direct imaging of the cell/substrate interface of thick specimens or imaging samples in turbid or opaque liquids since the optical path doesn’t pass through the sample. These results show promise that super-resolution chemical imaging may be used to differentiate chemical signatures from cells and could be applied to other biological structures of interest.Aeli P. OlsonKelsey B. SpiesAnna C. BrowningPaula A. G. SoneralNathan C. LindquistNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aeli P. Olson Kelsey B. Spies Anna C. Browning Paula A. G. Soneral Nathan C. Lindquist Chemically imaging bacteria with super-resolution SERS on ultra-thin silver substrates
description	Abstract Plasmonic hotspots generate a blinking Surface Enhanced Raman Spectroscopy (SERS) effect that can be processed using Stochastic Optical Reconstruction Microscopy (STORM) algorithms for super-resolved imaging. Furthermore, by imaging through a diffraction grating, STORM algorithms can be modified to extract a full SERS spectrum, thereby capturing spectral as well as spatial content simultaneously. Here we demonstrate SERS and STORM combined in this way for super-resolved chemical imaging using an ultra-thin silver substrate. Images of gram-positive and gram-negative bacteria taken with this technique show excellent agreement with scanning electron microscope images, high spatial resolution at <50 nm, and spectral SERS content that can be correlated to different regions. This may be used to identify unique chemical signatures of various cells. Finally, because we image through as-deposited, ultra-thin silver films, this technique requires no nanofabrication beyond a single deposition and looks at the cell samples from below. This allows direct imaging of the cell/substrate interface of thick specimens or imaging samples in turbid or opaque liquids since the optical path doesn’t pass through the sample. These results show promise that super-resolution chemical imaging may be used to differentiate chemical signatures from cells and could be applied to other biological structures of interest.
format	article
author	Aeli P. Olson Kelsey B. Spies Anna C. Browning Paula A. G. Soneral Nathan C. Lindquist
author_facet	Aeli P. Olson Kelsey B. Spies Anna C. Browning Paula A. G. Soneral Nathan C. Lindquist
author_sort	Aeli P. Olson
title	Chemically imaging bacteria with super-resolution SERS on ultra-thin silver substrates
title_short	Chemically imaging bacteria with super-resolution SERS on ultra-thin silver substrates
title_full	Chemically imaging bacteria with super-resolution SERS on ultra-thin silver substrates
title_fullStr	Chemically imaging bacteria with super-resolution SERS on ultra-thin silver substrates
title_full_unstemmed	Chemically imaging bacteria with super-resolution SERS on ultra-thin silver substrates
title_sort	chemically imaging bacteria with super-resolution sers on ultra-thin silver substrates
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9f820792733b4536b5fe44b5456ff652
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Chemically imaging bacteria with super-resolution SERS on ultra-thin silver substrates

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