Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium

Abstract Members of the yeast family of PUF proteins bind unique subsets of mRNA targets that encode proteins with common functions. They therefore became a paradigm for post-transcriptional gene control. To provide new insights into the roles of the seemingly redundant Puf1 and Puf2 members, we mon...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ofir Haramati, Anastasia Brodov, Idan Yelin, Avigail Atir-Lande, Nitzan Samra, Yoav Arava
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f8bca4a1bc24275acc305d13bf5a079
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9f8bca4a1bc24275acc305d13bf5a079
record_format	dspace
spelling	oai:doaj.org-article:9f8bca4a1bc24275acc305d13bf5a0792021-12-02T11:52:22ZIdentification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium10.1038/s41598-017-02873-z2045-2322https://doaj.org/article/9f8bca4a1bc24275acc305d13bf5a0792017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02873-zhttps://doaj.org/toc/2045-2322Abstract Members of the yeast family of PUF proteins bind unique subsets of mRNA targets that encode proteins with common functions. They therefore became a paradigm for post-transcriptional gene control. To provide new insights into the roles of the seemingly redundant Puf1 and Puf2 members, we monitored the growth rates of their deletions under many different stress conditions. A differential effect was observed at high CaCl2 concentrations, whereby puf1Δ growth was affected much more than puf2Δ, and inhibition was exacerbated in puf1Δpuf2Δ double knockout. Transcriptome analyses upon CaCl2 application for short and long terms defined the transcriptional response to CaCl2 and revealed distinct expression changes for the deletions. Intriguingly, mRNAs known to be bound by Puf1 or Puf2 were affected mainly in the double knockout. We focused on the cell wall regulator Zeo1 and observed that puf1Δpuf2Δ fails to maintain low levels of its mRNA. Complementarily, puf1Δpuf2Δ growth defect in CaCl2 was repaired upon further deletion of the Zeo1 gene. Thus, these proteins probably regulate the cell-wall integrity pathway by regulating Zeo1 post-transcriptionally. This work sheds new light on the roles of Puf proteins during the cellular response to environmental stress.Ofir HaramatiAnastasia BrodovIdan YelinAvigail Atir-LandeNitzan SamraYoav AravaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ofir Haramati Anastasia Brodov Idan Yelin Avigail Atir-Lande Nitzan Samra Yoav Arava Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium
description	Abstract Members of the yeast family of PUF proteins bind unique subsets of mRNA targets that encode proteins with common functions. They therefore became a paradigm for post-transcriptional gene control. To provide new insights into the roles of the seemingly redundant Puf1 and Puf2 members, we monitored the growth rates of their deletions under many different stress conditions. A differential effect was observed at high CaCl2 concentrations, whereby puf1Δ growth was affected much more than puf2Δ, and inhibition was exacerbated in puf1Δpuf2Δ double knockout. Transcriptome analyses upon CaCl2 application for short and long terms defined the transcriptional response to CaCl2 and revealed distinct expression changes for the deletions. Intriguingly, mRNAs known to be bound by Puf1 or Puf2 were affected mainly in the double knockout. We focused on the cell wall regulator Zeo1 and observed that puf1Δpuf2Δ fails to maintain low levels of its mRNA. Complementarily, puf1Δpuf2Δ growth defect in CaCl2 was repaired upon further deletion of the Zeo1 gene. Thus, these proteins probably regulate the cell-wall integrity pathway by regulating Zeo1 post-transcriptionally. This work sheds new light on the roles of Puf proteins during the cellular response to environmental stress.
format	article
author	Ofir Haramati Anastasia Brodov Idan Yelin Avigail Atir-Lande Nitzan Samra Yoav Arava
author_facet	Ofir Haramati Anastasia Brodov Idan Yelin Avigail Atir-Lande Nitzan Samra Yoav Arava
author_sort	Ofir Haramati
title	Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium
title_short	Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium
title_full	Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium
title_fullStr	Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium
title_full_unstemmed	Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium
title_sort	identification and characterization of roles for puf1 and puf2 proteins in the yeast response to high calcium
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9f8bca4a1bc24275acc305d13bf5a079
work_keys_str_mv	AT ofirharamati identificationandcharacterizationofrolesforpuf1andpuf2proteinsintheyeastresponsetohighcalcium AT anastasiabrodov identificationandcharacterizationofrolesforpuf1andpuf2proteinsintheyeastresponsetohighcalcium AT idanyelin identificationandcharacterizationofrolesforpuf1andpuf2proteinsintheyeastresponsetohighcalcium AT avigailatirlande identificationandcharacterizationofrolesforpuf1andpuf2proteinsintheyeastresponsetohighcalcium AT nitzansamra identificationandcharacterizationofrolesforpuf1andpuf2proteinsintheyeastresponsetohighcalcium AT yoavarava identificationandcharacterizationofrolesforpuf1andpuf2proteinsintheyeastresponsetohighcalcium
_version_	1718395060557447168

Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium

Ejemplares similares