Quantitative diffusion measurements using the open-source software PyFRAP

FRAP analysis often relies on simplified assumptions that can affect measurement accuracy. Here the authors present a Python-based FRAP analysis software using simulations instead of simplified theoretical models to fit the data, which accounts for complex sample geometries and bleach conditions.

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Autores principales: Alexander Bläßle, Gary Soh, Theresa Braun, David Mörsdorf, Hannes Preiß, Ben M. Jordan, Patrick Müller
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/9f8f9734505044aab1244113059f049a
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spelling oai:doaj.org-article:9f8f9734505044aab1244113059f049a2021-12-02T15:34:24ZQuantitative diffusion measurements using the open-source software PyFRAP10.1038/s41467-018-03975-62041-1723https://doaj.org/article/9f8f9734505044aab1244113059f049a2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03975-6https://doaj.org/toc/2041-1723FRAP analysis often relies on simplified assumptions that can affect measurement accuracy. Here the authors present a Python-based FRAP analysis software using simulations instead of simplified theoretical models to fit the data, which accounts for complex sample geometries and bleach conditions.Alexander BläßleGary SohTheresa BraunDavid MörsdorfHannes PreißBen M. JordanPatrick MüllerNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Alexander Bläßle
Gary Soh
Theresa Braun
David Mörsdorf
Hannes Preiß
Ben M. Jordan
Patrick Müller
Quantitative diffusion measurements using the open-source software PyFRAP
description FRAP analysis often relies on simplified assumptions that can affect measurement accuracy. Here the authors present a Python-based FRAP analysis software using simulations instead of simplified theoretical models to fit the data, which accounts for complex sample geometries and bleach conditions.
format article
author Alexander Bläßle
Gary Soh
Theresa Braun
David Mörsdorf
Hannes Preiß
Ben M. Jordan
Patrick Müller
author_facet Alexander Bläßle
Gary Soh
Theresa Braun
David Mörsdorf
Hannes Preiß
Ben M. Jordan
Patrick Müller
author_sort Alexander Bläßle
title Quantitative diffusion measurements using the open-source software PyFRAP
title_short Quantitative diffusion measurements using the open-source software PyFRAP
title_full Quantitative diffusion measurements using the open-source software PyFRAP
title_fullStr Quantitative diffusion measurements using the open-source software PyFRAP
title_full_unstemmed Quantitative diffusion measurements using the open-source software PyFRAP
title_sort quantitative diffusion measurements using the open-source software pyfrap
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/9f8f9734505044aab1244113059f049a
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