Transcriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice

Abstract We sequenced the transcriptome of brainstem interneurons in the specialized respiratory rhythmogenic site dubbed preBötzinger Complex (preBötC) from newborn mice. To distinguish molecular characteristics of the core oscillator we compared preBötC neurons derived from Dbx1-expressing progeni...

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Autores principales:	John A. Hayes, Andrew Kottick, Maria Cristina D. Picardo, Andrew D. Halleran, Ronald D. Smith, Gregory D. Smith, Margaret S. Saha, Christopher A. Del Negro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9f9bf901e346482b8ea557b69a56db98
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spelling	oai:doaj.org-article:9f9bf901e346482b8ea557b69a56db982021-12-02T15:05:51ZTranscriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice10.1038/s41598-017-09418-42045-2322https://doaj.org/article/9f9bf901e346482b8ea557b69a56db982017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09418-4https://doaj.org/toc/2045-2322Abstract We sequenced the transcriptome of brainstem interneurons in the specialized respiratory rhythmogenic site dubbed preBötzinger Complex (preBötC) from newborn mice. To distinguish molecular characteristics of the core oscillator we compared preBötC neurons derived from Dbx1-expressing progenitors that are respiratory rhythmogenic to neighbouring non-Dbx1-derived neurons, which support other respiratory and non-respiratory functions. Results in three categories are particularly salient. First, Dbx1 preBötC neurons express κ-opioid receptors in addition to μ-opioid receptors that heretofore have been associated with opiate respiratory depression, which may have clinical applications. Second, Dbx1 preBötC neurons express the hypoxia-inducible transcription factor Hif1a at levels three-times higher than non-Dbx1 neurons, which links core rhythmogenic microcircuits to O2-related chemosensation for the first time. Third, we detected a suite of transcription factors including Hoxa4 whose expression pattern may define the rostral preBötC border, Pbx3 that may influence ipsilateral connectivity, and Pax8 that may pertain to a ventrally-derived subset of Dbx1 preBötC neurons. These data establish the transcriptomic signature of the core respiratory oscillator at a perinatal stage of development.John A. HayesAndrew KottickMaria Cristina D. PicardoAndrew D. HalleranRonald D. SmithGregory D. SmithMargaret S. SahaChristopher A. Del NegroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John A. Hayes Andrew Kottick Maria Cristina D. Picardo Andrew D. Halleran Ronald D. Smith Gregory D. Smith Margaret S. Saha Christopher A. Del Negro Transcriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice
description	Abstract We sequenced the transcriptome of brainstem interneurons in the specialized respiratory rhythmogenic site dubbed preBötzinger Complex (preBötC) from newborn mice. To distinguish molecular characteristics of the core oscillator we compared preBötC neurons derived from Dbx1-expressing progenitors that are respiratory rhythmogenic to neighbouring non-Dbx1-derived neurons, which support other respiratory and non-respiratory functions. Results in three categories are particularly salient. First, Dbx1 preBötC neurons express κ-opioid receptors in addition to μ-opioid receptors that heretofore have been associated with opiate respiratory depression, which may have clinical applications. Second, Dbx1 preBötC neurons express the hypoxia-inducible transcription factor Hif1a at levels three-times higher than non-Dbx1 neurons, which links core rhythmogenic microcircuits to O2-related chemosensation for the first time. Third, we detected a suite of transcription factors including Hoxa4 whose expression pattern may define the rostral preBötC border, Pbx3 that may influence ipsilateral connectivity, and Pax8 that may pertain to a ventrally-derived subset of Dbx1 preBötC neurons. These data establish the transcriptomic signature of the core respiratory oscillator at a perinatal stage of development.
format	article
author	John A. Hayes Andrew Kottick Maria Cristina D. Picardo Andrew D. Halleran Ronald D. Smith Gregory D. Smith Margaret S. Saha Christopher A. Del Negro
author_facet	John A. Hayes Andrew Kottick Maria Cristina D. Picardo Andrew D. Halleran Ronald D. Smith Gregory D. Smith Margaret S. Saha Christopher A. Del Negro
author_sort	John A. Hayes
title	Transcriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice
title_short	Transcriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice
title_full	Transcriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice
title_fullStr	Transcriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice
title_full_unstemmed	Transcriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice
title_sort	transcriptome of neonatal prebötzinger complex neurones in dbx1 reporter mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9f9bf901e346482b8ea557b69a56db98
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Transcriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice

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