Gut Microbiome in Chronic Coronary Syndrome Patients
Despite knowledge of classical coronary artery disease (CAD) risk factors, the morbidity and mortality associated with this disease remain high. Therefore, new factors that may affect the development of CAD, such as the gut microbiome, are extensively investigated. This study aimed to evaluate gut m...
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MDPI AG
2021
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oai:doaj.org-article:9fbe5c938b9346fd981eb0048f6566c12021-11-11T17:41:50ZGut Microbiome in Chronic Coronary Syndrome Patients10.3390/jcm102150742077-0383https://doaj.org/article/9fbe5c938b9346fd981eb0048f6566c12021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/5074https://doaj.org/toc/2077-0383Despite knowledge of classical coronary artery disease (CAD) risk factors, the morbidity and mortality associated with this disease remain high. Therefore, new factors that may affect the development of CAD, such as the gut microbiome, are extensively investigated. This study aimed to evaluate gut microbiome composition in CAD patients in relation to the control group. We examined 169 CAD patients and 166 people in the control group, without CAD, matched in terms of age and sex to the study group. Both populations underwent a detailed health assessment. The microbiome analysis was based on the V3–V4 region of the 16S rRNA gene (NGS method). Among 4074 identified taxonomic units in the whole population, 1070 differed between study groups. The most common bacterial types were <i>Firmicutes</i>, <i>Bacteroidetes</i>, <i>Proteobacteria,</i> and <i>Actinobacteria</i>. Furthermore, a higher <i>Firmicutes/Bacteroidetes</i> ratio in the CAD group compared with the control was demonstrated. <i>Firmicutes/Bacteroidetes</i> ratio, independent of age, sex, CAD status, LDL cholesterol concentration, and statins treatment, was related to altered phosphatidylcholine concentrations obtained in targeted metabolomics. Altered alpha-biodiversity (Kruskal–Wallis test, <i>p</i> = 0.001) and beta-biodiversity (Bray–Curtis metric, <i>p</i> < 0.001) in the CAD group were observed. Moreover, a predicted functional analysis revealed some taxonomic units, metabolic pathways, and proteins that might be characteristic of the CAD patients’ microbiome, such as increased expressions of 6-phospho-β-glucosidase and protein-N(pi)-phosphohistidine-sugar phosphotransferase and decreased expressions of DNA topoisomerase, oxaloacetate decarboxylase, and 6-beta-glucosidase. In summary, CAD is associated with altered gut microbiome composition and function.Emilia Sawicka-SmiarowskaKinga BondarczukWitold BauerMagdalena NiemiraAnna SzalkowskaJustyna RaczkowskaMiroslaw KwasniewskiEwa TarasiukMarlena DubatowkaMagda LapinskaMalgorzata SzpakowiczZofia StachurskaAnna SzpakowiczPawel SowaAndrzej RaczkowskiMarcin KondraciukMagdalena GierejJoanna MotykaJacek JamiolkowskiMateusz BondarczukMalgorzata ChlabiczJolanta BuckoMarcin KozuchSlawomir DobrzyckiJerzy BychowskiWlodzimierz Jerzy MusialAdrian GodlewskiMichal CiborowskiAttila GyeneseiAdam KretowskiKarol Adam KaminskiMDPI AGarticlegut microbiomecoronary artery disease<i>Firmicutes/Bacteroidetes</i> ratiomicrobiome dysbiosistargeted metabolomicsphosphatidylcholineMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5074, p 5074 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
gut microbiome coronary artery disease <i>Firmicutes/Bacteroidetes</i> ratio microbiome dysbiosis targeted metabolomics phosphatidylcholine Medicine R |
| spellingShingle |
gut microbiome coronary artery disease <i>Firmicutes/Bacteroidetes</i> ratio microbiome dysbiosis targeted metabolomics phosphatidylcholine Medicine R Emilia Sawicka-Smiarowska Kinga Bondarczuk Witold Bauer Magdalena Niemira Anna Szalkowska Justyna Raczkowska Miroslaw Kwasniewski Ewa Tarasiuk Marlena Dubatowka Magda Lapinska Malgorzata Szpakowicz Zofia Stachurska Anna Szpakowicz Pawel Sowa Andrzej Raczkowski Marcin Kondraciuk Magdalena Gierej Joanna Motyka Jacek Jamiolkowski Mateusz Bondarczuk Malgorzata Chlabicz Jolanta Bucko Marcin Kozuch Slawomir Dobrzycki Jerzy Bychowski Wlodzimierz Jerzy Musial Adrian Godlewski Michal Ciborowski Attila Gyenesei Adam Kretowski Karol Adam Kaminski Gut Microbiome in Chronic Coronary Syndrome Patients |
| description |
Despite knowledge of classical coronary artery disease (CAD) risk factors, the morbidity and mortality associated with this disease remain high. Therefore, new factors that may affect the development of CAD, such as the gut microbiome, are extensively investigated. This study aimed to evaluate gut microbiome composition in CAD patients in relation to the control group. We examined 169 CAD patients and 166 people in the control group, without CAD, matched in terms of age and sex to the study group. Both populations underwent a detailed health assessment. The microbiome analysis was based on the V3–V4 region of the 16S rRNA gene (NGS method). Among 4074 identified taxonomic units in the whole population, 1070 differed between study groups. The most common bacterial types were <i>Firmicutes</i>, <i>Bacteroidetes</i>, <i>Proteobacteria,</i> and <i>Actinobacteria</i>. Furthermore, a higher <i>Firmicutes/Bacteroidetes</i> ratio in the CAD group compared with the control was demonstrated. <i>Firmicutes/Bacteroidetes</i> ratio, independent of age, sex, CAD status, LDL cholesterol concentration, and statins treatment, was related to altered phosphatidylcholine concentrations obtained in targeted metabolomics. Altered alpha-biodiversity (Kruskal–Wallis test, <i>p</i> = 0.001) and beta-biodiversity (Bray–Curtis metric, <i>p</i> < 0.001) in the CAD group were observed. Moreover, a predicted functional analysis revealed some taxonomic units, metabolic pathways, and proteins that might be characteristic of the CAD patients’ microbiome, such as increased expressions of 6-phospho-β-glucosidase and protein-N(pi)-phosphohistidine-sugar phosphotransferase and decreased expressions of DNA topoisomerase, oxaloacetate decarboxylase, and 6-beta-glucosidase. In summary, CAD is associated with altered gut microbiome composition and function. |
| format |
article |
| author |
Emilia Sawicka-Smiarowska Kinga Bondarczuk Witold Bauer Magdalena Niemira Anna Szalkowska Justyna Raczkowska Miroslaw Kwasniewski Ewa Tarasiuk Marlena Dubatowka Magda Lapinska Malgorzata Szpakowicz Zofia Stachurska Anna Szpakowicz Pawel Sowa Andrzej Raczkowski Marcin Kondraciuk Magdalena Gierej Joanna Motyka Jacek Jamiolkowski Mateusz Bondarczuk Malgorzata Chlabicz Jolanta Bucko Marcin Kozuch Slawomir Dobrzycki Jerzy Bychowski Wlodzimierz Jerzy Musial Adrian Godlewski Michal Ciborowski Attila Gyenesei Adam Kretowski Karol Adam Kaminski |
| author_facet |
Emilia Sawicka-Smiarowska Kinga Bondarczuk Witold Bauer Magdalena Niemira Anna Szalkowska Justyna Raczkowska Miroslaw Kwasniewski Ewa Tarasiuk Marlena Dubatowka Magda Lapinska Malgorzata Szpakowicz Zofia Stachurska Anna Szpakowicz Pawel Sowa Andrzej Raczkowski Marcin Kondraciuk Magdalena Gierej Joanna Motyka Jacek Jamiolkowski Mateusz Bondarczuk Malgorzata Chlabicz Jolanta Bucko Marcin Kozuch Slawomir Dobrzycki Jerzy Bychowski Wlodzimierz Jerzy Musial Adrian Godlewski Michal Ciborowski Attila Gyenesei Adam Kretowski Karol Adam Kaminski |
| author_sort |
Emilia Sawicka-Smiarowska |
| title |
Gut Microbiome in Chronic Coronary Syndrome Patients |
| title_short |
Gut Microbiome in Chronic Coronary Syndrome Patients |
| title_full |
Gut Microbiome in Chronic Coronary Syndrome Patients |
| title_fullStr |
Gut Microbiome in Chronic Coronary Syndrome Patients |
| title_full_unstemmed |
Gut Microbiome in Chronic Coronary Syndrome Patients |
| title_sort |
gut microbiome in chronic coronary syndrome patients |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/9fbe5c938b9346fd981eb0048f6566c1 |
| work_keys_str_mv |
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