Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume

Abstract Prunus mume (also known as Mei) is an important ornamental plant that is popular with Asians. The weeping trait in P. mume has attracted the attention of researchers for its high ornamental value. However, the formation of the weeping trait of woody plants is a complex process and the molec...

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Autores principales:	Lulu Li, Yichi Zhang, Tangchun Zheng, Xiaokang Zhuo, Ping Li, Like Qiu, Weichao Liu, Jia Wang, Tangren Cheng, Qixiang Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9fc4a2d1379b4f5e874c4ed62722a02a
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spelling	oai:doaj.org-article:9fc4a2d1379b4f5e874c4ed62722a02a2021-12-02T10:48:13ZComparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume10.1038/s41598-021-81892-32045-2322https://doaj.org/article/9fc4a2d1379b4f5e874c4ed62722a02a2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81892-3https://doaj.org/toc/2045-2322Abstract Prunus mume (also known as Mei) is an important ornamental plant that is popular with Asians. The weeping trait in P. mume has attracted the attention of researchers for its high ornamental value. However, the formation of the weeping trait of woody plants is a complex process and the molecular basis of weeping stem development is unclear. Here, the morphological and histochemical characteristics and transcriptome profiles of upright and weeping stems from P. mume were studied. Significant alterations in the histochemical characteristics of upright and weeping stems were observed, and the absence of phloem fibres and less xylem in weeping stems might be responsible for their inability to resist gravity and to grow downward. Transcriptome analysis showed that differentially expressed genes (DEGs) were enriched in phenylpropanoid biosynthesis and phytohormone signal transduction pathways. To investigate the differential responses to hormones, upright and weeping stems were treated with IAA (auxin) and GA3 (gibberellin A3), respectively, and the results revealed that weeping stems had a weaker IAA response ability and reduced upward bending angles than upright stems. On the contrary, weeping stems had increased upward bending angles than upright stems with GA3 treatment. Compared to upright stems, interestingly, DEGs associated with diterpenoid biosynthesis and phenylpropanoid biosynthesis were significantly enriched after being treated with IAA, and expression levels of genes associated with phenylpropanoid biosynthesis, ABC transporters, glycosylphosphatidylinositol (GPI)—anchor biosynthesis were altered after being treated with GA3 in weeping stems. Those results reveal that multiple molecular mechanisms regulate the formation of weeping trait in P. mume, which lays a theoretical foundation for the cultivation of new varieties.Lulu LiYichi ZhangTangchun ZhengXiaokang ZhuoPing LiLike QiuWeichao LiuJia WangTangren ChengQixiang ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lulu Li Yichi Zhang Tangchun Zheng Xiaokang Zhuo Ping Li Like Qiu Weichao Liu Jia Wang Tangren Cheng Qixiang Zhang Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume
description	Abstract Prunus mume (also known as Mei) is an important ornamental plant that is popular with Asians. The weeping trait in P. mume has attracted the attention of researchers for its high ornamental value. However, the formation of the weeping trait of woody plants is a complex process and the molecular basis of weeping stem development is unclear. Here, the morphological and histochemical characteristics and transcriptome profiles of upright and weeping stems from P. mume were studied. Significant alterations in the histochemical characteristics of upright and weeping stems were observed, and the absence of phloem fibres and less xylem in weeping stems might be responsible for their inability to resist gravity and to grow downward. Transcriptome analysis showed that differentially expressed genes (DEGs) were enriched in phenylpropanoid biosynthesis and phytohormone signal transduction pathways. To investigate the differential responses to hormones, upright and weeping stems were treated with IAA (auxin) and GA3 (gibberellin A3), respectively, and the results revealed that weeping stems had a weaker IAA response ability and reduced upward bending angles than upright stems. On the contrary, weeping stems had increased upward bending angles than upright stems with GA3 treatment. Compared to upright stems, interestingly, DEGs associated with diterpenoid biosynthesis and phenylpropanoid biosynthesis were significantly enriched after being treated with IAA, and expression levels of genes associated with phenylpropanoid biosynthesis, ABC transporters, glycosylphosphatidylinositol (GPI)—anchor biosynthesis were altered after being treated with GA3 in weeping stems. Those results reveal that multiple molecular mechanisms regulate the formation of weeping trait in P. mume, which lays a theoretical foundation for the cultivation of new varieties.
format	article
author	Lulu Li Yichi Zhang Tangchun Zheng Xiaokang Zhuo Ping Li Like Qiu Weichao Liu Jia Wang Tangren Cheng Qixiang Zhang
author_facet	Lulu Li Yichi Zhang Tangchun Zheng Xiaokang Zhuo Ping Li Like Qiu Weichao Liu Jia Wang Tangren Cheng Qixiang Zhang
author_sort	Lulu Li
title	Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume
title_short	Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume
title_full	Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume
title_fullStr	Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume
title_full_unstemmed	Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume
title_sort	comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in prunus mume
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9fc4a2d1379b4f5e874c4ed62722a02a
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Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume

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