Transcriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds

Abstract The zanthoxylum seeds are oil-rich and have a very thick, dense and oily shell. In the natural conditions the seeds have a very low germination rate. Prior to treatment with GAs to promote germination, the seeds were usually soaked in sulfuric acid to remove shells easily. A high-throughput...

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Autores principales:	Jikang Sun, Ping Wang, Tao Zhou, Jian Rong, Hao Jia, Zhiming Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9fc616386f814c12b7e74dc3c12a3370
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spelling	oai:doaj.org-article:9fc616386f814c12b7e74dc3c12a33702021-12-02T12:31:46ZTranscriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds10.1038/s41598-017-07424-02045-2322https://doaj.org/article/9fc616386f814c12b7e74dc3c12a33702017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07424-0https://doaj.org/toc/2045-2322Abstract The zanthoxylum seeds are oil-rich and have a very thick, dense and oily shell. In the natural conditions the seeds have a very low germination rate. Prior to treatment with GAs to promote germination, the seeds were usually soaked in sulfuric acid to remove shells easily. A high-throughput sequencing of mRNAs was performed to investigate the effects of the above treatments on the germination of zanthoxylum seeds. Seven libraries were assembled into 100,982 unigenes and 59,509 unigenes were annotated. We focused on the expression profiles of the key genes related to the oil metabolisms and hormone regulations during seed germination. Our data indicated the endogenous ABA of seeds was rich. The effects that the exogenous GAs promoted germination were apparent in the secong day of germination. Especially, for the first time our results indicated the exogenous GAs lowered the aerobic metabolism including the oil metabolisms during imbibition. We inferred that the exogenous GAs had inhibitory effects on the oil metabolisms to avoide oxidative damages to the imbibed seeds, and the seed shell played the role similiar to the exogenous GAs in the initial stage of germination in the natural conditions.Jikang SunPing WangTao ZhouJian RongHao JiaZhiming LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jikang Sun Ping Wang Tao Zhou Jian Rong Hao Jia Zhiming Liu Transcriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds
description	Abstract The zanthoxylum seeds are oil-rich and have a very thick, dense and oily shell. In the natural conditions the seeds have a very low germination rate. Prior to treatment with GAs to promote germination, the seeds were usually soaked in sulfuric acid to remove shells easily. A high-throughput sequencing of mRNAs was performed to investigate the effects of the above treatments on the germination of zanthoxylum seeds. Seven libraries were assembled into 100,982 unigenes and 59,509 unigenes were annotated. We focused on the expression profiles of the key genes related to the oil metabolisms and hormone regulations during seed germination. Our data indicated the endogenous ABA of seeds was rich. The effects that the exogenous GAs promoted germination were apparent in the secong day of germination. Especially, for the first time our results indicated the exogenous GAs lowered the aerobic metabolism including the oil metabolisms during imbibition. We inferred that the exogenous GAs had inhibitory effects on the oil metabolisms to avoide oxidative damages to the imbibed seeds, and the seed shell played the role similiar to the exogenous GAs in the initial stage of germination in the natural conditions.
format	article
author	Jikang Sun Ping Wang Tao Zhou Jian Rong Hao Jia Zhiming Liu
author_facet	Jikang Sun Ping Wang Tao Zhou Jian Rong Hao Jia Zhiming Liu
author_sort	Jikang Sun
title	Transcriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds
title_short	Transcriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds
title_full	Transcriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds
title_fullStr	Transcriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds
title_full_unstemmed	Transcriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds
title_sort	transcriptome analysis of the effects of shell removal and exogenous gibberellin on germination of zanthoxylum seeds
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9fc616386f814c12b7e74dc3c12a3370
work_keys_str_mv	AT jikangsun transcriptomeanalysisoftheeffectsofshellremovalandexogenousgibberellinongerminationofzanthoxylumseeds AT pingwang transcriptomeanalysisoftheeffectsofshellremovalandexogenousgibberellinongerminationofzanthoxylumseeds AT taozhou transcriptomeanalysisoftheeffectsofshellremovalandexogenousgibberellinongerminationofzanthoxylumseeds AT jianrong transcriptomeanalysisoftheeffectsofshellremovalandexogenousgibberellinongerminationofzanthoxylumseeds AT haojia transcriptomeanalysisoftheeffectsofshellremovalandexogenousgibberellinongerminationofzanthoxylumseeds AT zhimingliu transcriptomeanalysisoftheeffectsofshellremovalandexogenousgibberellinongerminationofzanthoxylumseeds
_version_	1718394295779590144

Transcriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds

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