Histological Tracking into the Third Dimension: Evolution of Early Tumorigenesis in VHL Kidney

Using a novel three-dimensional (3D) approach, we tracked histological changes to elucidate the earliest stages of renal clear cell neoplasia in normal kidney tissue of patients with von Hippel-Lindau (VHL) disease. Tissue blocks of interest were procured, serially sectioned, and 3D reconstruction...

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Autores principales:	Mayyan Mubarak, Nayef Al-Gharaibeh, Samuel Sommaruga, Jie Li, Alexander Oliver Vortmeyer
Formato:	article
Lenguaje:	EN
Publicado:	Codon Publications 2021
Materias:	VHL renal clear cell carcinoma clear cell carcinogenesis Diseases of the genitourinary system. Urology RC870-923 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/9fc74aa2e10344159dff30bc9042f14c
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spelling	oai:doaj.org-article:9fc74aa2e10344159dff30bc9042f14c2021-11-10T00:52:10ZHistological Tracking into the Third Dimension: Evolution of Early Tumorigenesis in VHL Kidney10.15586/jkcvhl.v8i3.1892203-5826https://doaj.org/article/9fc74aa2e10344159dff30bc9042f14c2021-09-01T00:00:00Zhttps://jkcvhl.com/~jkcvhlco/index.php/jkcvhl/article/view/189https://doaj.org/toc/2203-5826 Using a novel three-dimensional (3D) approach, we tracked histological changes to elucidate the earliest stages of renal clear cell neoplasia in normal kidney tissue of patients with von Hippel-Lindau (VHL) disease. Tissue blocks of interest were procured, serially sectioned, and 3D reconstruction of the entirety of pathologic events was performed. The results reveal an abundance of foci with aberrant clear cell proliferation that initially develop along the tubular lining, but have the potential to aggregate within individual tubules. This stage is followed by the extension of clear cell aggregates beyond the tubular basement membrane, which allows for the recruitment of angiogenesis derived from interstitial vasculature. The results suggest that the most frequent pathologic event in VHL kidneys is the presence of isolated or aggregated clear cells within the tubular epithelium, potentially developing further into a protracted process of neoplasia. The abundance of independent pathologic events in VHL kidneys confirms developmental mechanisms to precede tumor initiation. To our knowledge, this is the first report demonstrating that tracking of histologic changes in the 3rd dimension enables the confirmation of the sequence of events from the earliest pathologic change in the VHL kidney to the neoplastic stage. This approach is not only useful for visualization and quantification of pathologic changes but also for targeted sampling allowing selective analysis of the earliest stages of clear cell carcinogenesis. Mayyan MubarakNayef Al-GharaibehSamuel SommarugaJie LiAlexander Oliver VortmeyerCodon PublicationsarticleVHLrenal clear cell carcinomaclear cellcarcinogenesisDiseases of the genitourinary system. UrologyRC870-923Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENJournal of Kidney Cancer and VHL, Vol 8, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	VHL renal clear cell carcinoma clear cell carcinogenesis Diseases of the genitourinary system. Urology RC870-923 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	VHL renal clear cell carcinoma clear cell carcinogenesis Diseases of the genitourinary system. Urology RC870-923 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Mayyan Mubarak Nayef Al-Gharaibeh Samuel Sommaruga Jie Li Alexander Oliver Vortmeyer Histological Tracking into the Third Dimension: Evolution of Early Tumorigenesis in VHL Kidney
description	Using a novel three-dimensional (3D) approach, we tracked histological changes to elucidate the earliest stages of renal clear cell neoplasia in normal kidney tissue of patients with von Hippel-Lindau (VHL) disease. Tissue blocks of interest were procured, serially sectioned, and 3D reconstruction of the entirety of pathologic events was performed. The results reveal an abundance of foci with aberrant clear cell proliferation that initially develop along the tubular lining, but have the potential to aggregate within individual tubules. This stage is followed by the extension of clear cell aggregates beyond the tubular basement membrane, which allows for the recruitment of angiogenesis derived from interstitial vasculature. The results suggest that the most frequent pathologic event in VHL kidneys is the presence of isolated or aggregated clear cells within the tubular epithelium, potentially developing further into a protracted process of neoplasia. The abundance of independent pathologic events in VHL kidneys confirms developmental mechanisms to precede tumor initiation. To our knowledge, this is the first report demonstrating that tracking of histologic changes in the 3rd dimension enables the confirmation of the sequence of events from the earliest pathologic change in the VHL kidney to the neoplastic stage. This approach is not only useful for visualization and quantification of pathologic changes but also for targeted sampling allowing selective analysis of the earliest stages of clear cell carcinogenesis.
format	article
author	Mayyan Mubarak Nayef Al-Gharaibeh Samuel Sommaruga Jie Li Alexander Oliver Vortmeyer
author_facet	Mayyan Mubarak Nayef Al-Gharaibeh Samuel Sommaruga Jie Li Alexander Oliver Vortmeyer
author_sort	Mayyan Mubarak
title	Histological Tracking into the Third Dimension: Evolution of Early Tumorigenesis in VHL Kidney
title_short	Histological Tracking into the Third Dimension: Evolution of Early Tumorigenesis in VHL Kidney
title_full	Histological Tracking into the Third Dimension: Evolution of Early Tumorigenesis in VHL Kidney
title_fullStr	Histological Tracking into the Third Dimension: Evolution of Early Tumorigenesis in VHL Kidney
title_full_unstemmed	Histological Tracking into the Third Dimension: Evolution of Early Tumorigenesis in VHL Kidney
title_sort	histological tracking into the third dimension: evolution of early tumorigenesis in vhl kidney
publisher	Codon Publications
publishDate	2021
url	https://doaj.org/article/9fc74aa2e10344159dff30bc9042f14c
work_keys_str_mv	AT mayyanmubarak histologicaltrackingintothethirddimensionevolutionofearlytumorigenesisinvhlkidney AT nayefalgharaibeh histologicaltrackingintothethirddimensionevolutionofearlytumorigenesisinvhlkidney AT samuelsommaruga histologicaltrackingintothethirddimensionevolutionofearlytumorigenesisinvhlkidney AT jieli histologicaltrackingintothethirddimensionevolutionofearlytumorigenesisinvhlkidney AT alexanderolivervortmeyer histologicaltrackingintothethirddimensionevolutionofearlytumorigenesisinvhlkidney
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Histological Tracking into the Third Dimension: Evolution of Early Tumorigenesis in VHL Kidney

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