Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions

Conventional reconstruction methods used in cryo-EM single particle analysis do not take the depth of field effect into account. Here the authors present a block-based reconstruction method to deal with the depth of field effect and show that this approach can improve the resolution of cryo-EM virus...

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Autores principales:	Dongjie Zhu, Xiangxi Wang, Qianglin Fang, James L Van Etten, Michael G Rossmann, Zihe Rao, Xinzheng Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9fdaf977a55d494ead3a7b296f782fd3
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spelling	oai:doaj.org-article:9fdaf977a55d494ead3a7b296f782fd32021-12-02T14:40:44ZPushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions10.1038/s41467-018-04051-92041-1723https://doaj.org/article/9fdaf977a55d494ead3a7b296f782fd32018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04051-9https://doaj.org/toc/2041-1723Conventional reconstruction methods used in cryo-EM single particle analysis do not take the depth of field effect into account. Here the authors present a block-based reconstruction method to deal with the depth of field effect and show that this approach can improve the resolution of cryo-EM virus structures.Dongjie ZhuXiangxi WangQianglin FangJames L Van EttenMichael G RossmannZihe RaoXinzheng ZhangNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dongjie Zhu Xiangxi Wang Qianglin Fang James L Van Etten Michael G Rossmann Zihe Rao Xinzheng Zhang Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
description	Conventional reconstruction methods used in cryo-EM single particle analysis do not take the depth of field effect into account. Here the authors present a block-based reconstruction method to deal with the depth of field effect and show that this approach can improve the resolution of cryo-EM virus structures.
format	article
author	Dongjie Zhu Xiangxi Wang Qianglin Fang James L Van Etten Michael G Rossmann Zihe Rao Xinzheng Zhang
author_facet	Dongjie Zhu Xiangxi Wang Qianglin Fang James L Van Etten Michael G Rossmann Zihe Rao Xinzheng Zhang
author_sort	Dongjie Zhu
title	Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title_short	Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title_full	Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title_fullStr	Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title_full_unstemmed	Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title_sort	pushing the resolution limit by correcting the ewald sphere effect in single-particle cryo-em reconstructions
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9fdaf977a55d494ead3a7b296f782fd3
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Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions

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