Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm [version 1; peer review: 2 approved]

Background: Natural products with antibacterial potential have begun to be tested on biofilm models, bringing us closer to understanding the response generated by the complex microbial ecosystems of the oral cavity. The objective of this study was to evaluate the antibacterial, antibiofilm, and cyto...

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Autores principales:	Pablo Alejandro Millones Gómez, Lidia Yileng Tay Chu Jon, Dora Jesús Maurtua Torres, Reyma Evelyn Bacilio Amaranto, Ingrit Elida Collantes Díaz, Carlos Alberto Minchón Medina
Formato:	article
Lenguaje:	EN
Publicado:	F1000 Research Ltd 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9fe128fe7c914c42ab5644ebbd358805
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spelling	oai:doaj.org-article:9fe128fe7c914c42ab5644ebbd3588052021-11-29T14:03:15ZAntibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm [version 1; peer review: 2 approved]2046-140210.12688/f1000research.73602.1https://doaj.org/article/9fe128fe7c914c42ab5644ebbd3588052021-10-01T00:00:00Zhttps://f1000research.com/articles/10-1093/v1https://doaj.org/toc/2046-1402Background: Natural products with antibacterial potential have begun to be tested on biofilm models, bringing us closer to understanding the response generated by the complex microbial ecosystems of the oral cavity. The objective of this study was to evaluate the antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro biofilm of Streptococcus gordonii and Fusobacterium nucleatum. Methods: The experimental work involved a consecutive, in vitro, longitudinal, and double-blinded study design. Propolis samples were collected from 13 different regions of the Peruvian Andes. The disk diffusion method was used for the antimicrobial susceptibility test. The cytotoxic effect of propolis on human gingival fibroblasts was determined by cell viability method using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, and the effect of propolis on the biofilm was evaluated by confocal microscopy and polymerase chain reaction (PCR). Results: The 0.78 mg/mL and 1.563 mg/mL concentrations of the methanolic fraction of the chloroform residue of Oxapampa propolis showed effects on biofilm thickness and the copy numbers of the srtA gene of S. gordonii and the radD gene of F. nucleatum at 48 and 120 hours, and chromatography (UV, λ 280 nm) identified rhamnocitrin, isorhamnetin, apigenin, kaempferol, diosmetin, acacetin, glycerol, and chrysoeriol. Conclusions: Of the 13 propolis evaluated, it was found that only the methanolic fraction of Oxapampa propolis showed antibacterial and antibiofilm effects without causing damage to human gingival fibroblasts. Likewise, when evaluating the chemical composition of this fraction, eight flavonoids were identified.Pablo Alejandro Millones GómezLidia Yileng Tay Chu JonDora Jesús Maurtua TorresReyma Evelyn Bacilio AmarantoIngrit Elida Collantes DíazCarlos Alberto Minchón MedinaF1000 Research LtdarticleMedicineRScienceQENF1000Research, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pablo Alejandro Millones Gómez Lidia Yileng Tay Chu Jon Dora Jesús Maurtua Torres Reyma Evelyn Bacilio Amaranto Ingrit Elida Collantes Díaz Carlos Alberto Minchón Medina Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm [version 1; peer review: 2 approved]
description	Background: Natural products with antibacterial potential have begun to be tested on biofilm models, bringing us closer to understanding the response generated by the complex microbial ecosystems of the oral cavity. The objective of this study was to evaluate the antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro biofilm of Streptococcus gordonii and Fusobacterium nucleatum. Methods: The experimental work involved a consecutive, in vitro, longitudinal, and double-blinded study design. Propolis samples were collected from 13 different regions of the Peruvian Andes. The disk diffusion method was used for the antimicrobial susceptibility test. The cytotoxic effect of propolis on human gingival fibroblasts was determined by cell viability method using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, and the effect of propolis on the biofilm was evaluated by confocal microscopy and polymerase chain reaction (PCR). Results: The 0.78 mg/mL and 1.563 mg/mL concentrations of the methanolic fraction of the chloroform residue of Oxapampa propolis showed effects on biofilm thickness and the copy numbers of the srtA gene of S. gordonii and the radD gene of F. nucleatum at 48 and 120 hours, and chromatography (UV, λ 280 nm) identified rhamnocitrin, isorhamnetin, apigenin, kaempferol, diosmetin, acacetin, glycerol, and chrysoeriol. Conclusions: Of the 13 propolis evaluated, it was found that only the methanolic fraction of Oxapampa propolis showed antibacterial and antibiofilm effects without causing damage to human gingival fibroblasts. Likewise, when evaluating the chemical composition of this fraction, eight flavonoids were identified.
format	article
author	Pablo Alejandro Millones Gómez Lidia Yileng Tay Chu Jon Dora Jesús Maurtua Torres Reyma Evelyn Bacilio Amaranto Ingrit Elida Collantes Díaz Carlos Alberto Minchón Medina
author_facet	Pablo Alejandro Millones Gómez Lidia Yileng Tay Chu Jon Dora Jesús Maurtua Torres Reyma Evelyn Bacilio Amaranto Ingrit Elida Collantes Díaz Carlos Alberto Minchón Medina
author_sort	Pablo Alejandro Millones Gómez
title	Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm [version 1; peer review: 2 approved]
title_short	Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm [version 1; peer review: 2 approved]
title_full	Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm [version 1; peer review: 2 approved]
title_fullStr	Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm [version 1; peer review: 2 approved]
title_full_unstemmed	Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm [version 1; peer review: 2 approved]
title_sort	antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of peruvian propolis in an in vitro oral biofilm [version 1; peer review: 2 approved]
publisher	F1000 Research Ltd
publishDate	2021
url	https://doaj.org/article/9fe128fe7c914c42ab5644ebbd358805
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Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm [version 1; peer review: 2 approved]

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