Soil biochar amendment affects the diversity of nosZ transcripts: Implications for N2O formation

Abstract Microbial nitrogen transformation processes such as denitrification represent major sources of the potent greenhouse gas nitrous oxide (N2O). Soil biochar amendment has been shown to significantly decrease N2O emissions in various soils. However, the effect of biochar on the structure and f...

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Autores principales:	Johannes Harter, Mohamed El-Hadidi, Daniel H. Huson, Andreas Kappler, Sebastian Behrens
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9fe1ab49dba342d39cf6f785862c9457
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spelling	oai:doaj.org-article:9fe1ab49dba342d39cf6f785862c94572021-12-02T12:32:51ZSoil biochar amendment affects the diversity of nosZ transcripts: Implications for N2O formation10.1038/s41598-017-03282-y2045-2322https://doaj.org/article/9fe1ab49dba342d39cf6f785862c94572017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03282-yhttps://doaj.org/toc/2045-2322Abstract Microbial nitrogen transformation processes such as denitrification represent major sources of the potent greenhouse gas nitrous oxide (N2O). Soil biochar amendment has been shown to significantly decrease N2O emissions in various soils. However, the effect of biochar on the structure and function of microbial communities that actively perform nitrogen redox transformations has not been studied in detail yet. To analyse the community composition of actively denitrifying and N2O-reducing microbial communities, we collected RNA samples at different time points from a soil microcosm experiment conducted under denitrifying conditions and performed Illumina amplicon sequencing targeting nirK, typical nosZ and atypical nosZ mRNA transcripts. Within 10 days, biochar significantly increased the diversity of nirK and typical nosZ transcripts and resulted in taxonomic shifts among the typical nosZ-expressing microbial community. Furthermore, biochar addition led to a significant increase in transcript production among microbial species that are specialized on direct N2O reduction from the environment. Our results point towards a potential coupling of biochar-induced N2O emission reduction and an increase in microbial N2O reduction activity among specific groups of typical and atypical N2O reducers. However, experiments with other soils and biochars will be required to verify the transferability of these findings to other soil-biochar systems.Johannes HarterMohamed El-HadidiDaniel H. HusonAndreas KapplerSebastian BehrensNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johannes Harter Mohamed El-Hadidi Daniel H. Huson Andreas Kappler Sebastian Behrens Soil biochar amendment affects the diversity of nosZ transcripts: Implications for N2O formation
description	Abstract Microbial nitrogen transformation processes such as denitrification represent major sources of the potent greenhouse gas nitrous oxide (N2O). Soil biochar amendment has been shown to significantly decrease N2O emissions in various soils. However, the effect of biochar on the structure and function of microbial communities that actively perform nitrogen redox transformations has not been studied in detail yet. To analyse the community composition of actively denitrifying and N2O-reducing microbial communities, we collected RNA samples at different time points from a soil microcosm experiment conducted under denitrifying conditions and performed Illumina amplicon sequencing targeting nirK, typical nosZ and atypical nosZ mRNA transcripts. Within 10 days, biochar significantly increased the diversity of nirK and typical nosZ transcripts and resulted in taxonomic shifts among the typical nosZ-expressing microbial community. Furthermore, biochar addition led to a significant increase in transcript production among microbial species that are specialized on direct N2O reduction from the environment. Our results point towards a potential coupling of biochar-induced N2O emission reduction and an increase in microbial N2O reduction activity among specific groups of typical and atypical N2O reducers. However, experiments with other soils and biochars will be required to verify the transferability of these findings to other soil-biochar systems.
format	article
author	Johannes Harter Mohamed El-Hadidi Daniel H. Huson Andreas Kappler Sebastian Behrens
author_facet	Johannes Harter Mohamed El-Hadidi Daniel H. Huson Andreas Kappler Sebastian Behrens
author_sort	Johannes Harter
title	Soil biochar amendment affects the diversity of nosZ transcripts: Implications for N2O formation
title_short	Soil biochar amendment affects the diversity of nosZ transcripts: Implications for N2O formation
title_full	Soil biochar amendment affects the diversity of nosZ transcripts: Implications for N2O formation
title_fullStr	Soil biochar amendment affects the diversity of nosZ transcripts: Implications for N2O formation
title_full_unstemmed	Soil biochar amendment affects the diversity of nosZ transcripts: Implications for N2O formation
title_sort	soil biochar amendment affects the diversity of nosz transcripts: implications for n2o formation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9fe1ab49dba342d39cf6f785862c9457
work_keys_str_mv	AT johannesharter soilbiocharamendmentaffectsthediversityofnosztranscriptsimplicationsforn2oformation AT mohamedelhadidi soilbiocharamendmentaffectsthediversityofnosztranscriptsimplicationsforn2oformation AT danielhhuson soilbiocharamendmentaffectsthediversityofnosztranscriptsimplicationsforn2oformation AT andreaskappler soilbiocharamendmentaffectsthediversityofnosztranscriptsimplicationsforn2oformation AT sebastianbehrens soilbiocharamendmentaffectsthediversityofnosztranscriptsimplicationsforn2oformation
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Soil biochar amendment affects the diversity of nosZ transcripts: Implications for N2O formation

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